Search

Bentonite-based composites have been widely utilized in the removal of various pollutants due to low cost, environmentally friendly, ease-to-operate, whereas the recent advances concerning the application of bentonite-based composites in environmental remediation were not available. Herein, the modification (i.e., acid/alkaline washing, thermal treatment and hybrids) of bentonite was firstly reviewed; Then the recent advances of adsorption of environmental concomitants (e.g., organic (dyes, microplastics, phenolic and other organics) and inorganic pollutants (heavy metals, radionuclides and other inorganic pollutants)) on various bentonite-based composites were summarized in details. Meanwhile, the effect of environmental factors and interaction mechanism between bentonite-based composites and contaminants were also investigated. Finally, the conclusions and prospective of bentonite-based composites in the environmental remediation were proposed. It is demonstrated that various bentonite-based composites exhibited the high adsorption/degradation capacity towards environmental pollutants under the specific conditions. The interaction mechanism involved the mineralization, physical/chemical adsorption, co-precipitation and complexation. This review highlights the effect of different functionalization of bentonite-based composites on their adsorption capacity and interaction mechanism, which is expected to be helpful to environmental scientists for applying bentonite-based composites into practical environmental remediation. • The modification of bentonite was firstly reviewed. • Recent advances of adsorption of organics, heavy metals and radionuclides on bentonite were summarized. • Interaction mechanism of bentonite and contaminants were investigated. • The conclusions and prospective of bentonite-based composites were proposed. • This review is expected to be helpful into practical environmental remediation. [ABSTRACT FROM AUTHOR]

Biochar, with its dual roles of soil remediation and carbon sequestration, is gradually demonstrating great potential for sustainability in agricultural and ecological aspects. In this study, a porous biochar derived from walnut shell wastes was prepared via a facile pyrolysis coupling with in-situ alkali etching method. An incubation study was conducted to investigate its performance in stabilizing copper (Cu) and lead (Pb) co-contaminated soils under different utilization types. The biochar effectively decreased the bioavailable Cu (8.5–91.68%) and Pb (5.03–88.54%), while increasing the pH, CEC, and SOM contents in both soils. Additionally, the results of sequential extraction confirmed that biochar promoted the transformation of the labile fraction of Cu and Pb to stable fractions. The mechanisms of Cu and Pb stabilization were found to be greatly dependent on the soil types. For tea plantation yellow soil, the main approach for stabilization was the complexation of heavy metals with abundant organic functional groups and deprotonation structure. Surface electrostatic adsorption and cation exchange contributed to the immobilization of Cu and Pb in vegetable-cultivated purple soil. This research provides valuable information for the stabilization of Cu and Pb co-contaminated soils for different utilization types using environmentally-friendly biochar. • Biochar was prepared via a facile pyrolysis coupling with in-situ alkali etching method. • The biochar amendment effectively decreased the bioavailable Cu and Pb in soil. • Cu and Pb stabilization depended on soil properties especially influenced by land use. • SOM was the main influence factor of Cu and Pb immobilization for tea planted soil. • PH and CEC markedly contributed to the stability of Cu and Pb in vegetable cultivated soil. [ABSTRACT FROM AUTHOR]

Heavy metal pollution in farmland soil has become increasingly severe, and multi-element composite pollution has brought enormous harm to human production and life. Environmental changes in cold regions (such as freeze-thaw cycles and dry-wet alternations) may increase the potential physiological toxicity of heavy metals and exacerbate pollution risks. In order to reveal the effectiveness of sepiolite modified biochar in the remediation of the soil contaminated with lead (Pb), cadmium (Cd), and chromium (Cr), the rice husk biochar pyrolyzed at 500 and 800 °C were selected for remediation treatment (denoted as BC500 and BC800). Meanwhile, different proportions of sepiolite were used for modification (biochar: sepiolite = 1: 0.5 and 1: 1), denoted as MBC500/MBC800 and HBC500/HBC800, respectively. The results showed that modified biochar with sepiolite can effectively improve the immobilization of heavy metals. Under natural conservation condition, the amount of diethylenetriaminepentaacetic acid (DTPA) extractable Pb in BC500, MBC500, and HBC500 decreased by 5.95, 12.39, and 13.55%, respectively, compared to CK. Freeze-thaw cycles and dry-wet alternations activated soil heavy metals, while modified biochar increased adsorption sites and oxygen-containing functional groups under aging conditions, inhibiting the fractions transformation of heavy metals. Furthermore, freeze-thaw cycles promoted the decomposition and mineralization of soil organic carbon (SOC), while sepiolite hindered the release of active carbon through ion exchange and adsorption complexation. Among them, and the soil dissolved organic carbon (DOC) content in HBC800 decreased by 49.39% compared to BC800. Additionally, the high-temperature pyrolyzed biochar (BC800) enhanced the porosity richness and alkalinity of material, which effectively inhibited the migration and transformation of heavy metals compared to BC500, and reduced the decomposition of soil DOC. [Display omitted] • Freeze-thaw cycles and dry-wet alternation enhanced the activity of heavy metals. • Biochar effectively passivated and immobilized heavy metals (Pb, Cd and Cr). • Sepiolite inhibited the release of carbon through adsorption and complexation. • High temperature pyrolysis biochar enhances the immobilization of metals. • Modified biochar is beneficial for reducing pollution and carbon emissions. [ABSTRACT FROM AUTHOR]

Heavy metal pollution of agricultural soil is a major global concern, prompting the establishment of maximum allowable limits (MALs) to ensure food safety and protect human health. This study collected and compared MALs for six heavy metals (As, Cd, Hg, Pb, Zn, and Cu) in agricultural soils from representative countries and organizations (EU and WHO/FAO). The research evaluated the critical health risks and efficacy of these MALs under the hypothetical scenario of metals concentrations reaching the maximum allowable level. Safe thresholds for heavy metals were then derived based on maximum acceptable health risk levels. The comparative analysis revealed significant variations in the specific limit values and terms of MALs across countries and organizations, even for the same metal. This suggests that there is no consensus among countries and organizations regarding the level of metal-related health risks. Furthermore, the risk analysis of metal concentrations reaching the maximum level accentuated heightened risks associated with As, suggesting that the current risk of soil As exposure was underestimated, particularly for children. However, soil Cu, Cd, and Zn limits generally resulted in low health risks, implying that the current limits may overestimate their hazard. Overall, the results highlight that the current MALs for soil heavy metals may not fully safeguard human health. There is a critical need to optimize current soil MALs based on localized risks and the actual impact of these metals on human health. It is suggested to appropriately lower the limits of metals (such as As) whose impact on health risks is underestimated, and cautiously increase the limits of metals (such as Cu, Cd, and Zn) that currently pose minor health risks. This approach aims to reduce both over and insufficient protection problems of soil heavy metal MALs, emphasizing the importance of considering the locality in setting these limits. [Display omitted] • A health risk assessment of heavy metals limits for farmland was conducted. • Thresholds for metals in farmland were backward derived by the acceptable risk. • Terms and values of metals limits for farmland exhibited substantial discrepancies. • Most As limits may be unprotective while other metals limits are conservative. • A deeper exploration of soil exposure and metals toxicity helps to update limits. [ABSTRACT FROM AUTHOR]

The speciation of Cr, Zn, Cu and Pb in two metal finishing filter cakes (TX and ST) was investigated by X-ray absorption spectroscopy (XAS) complemented by X-ray fluorescence (XRF) and X-ray diffraction (XRD). XRF showed that concentrations of Cr, Zn, Cu and Pb were 1.4%, 0.19%, 0.20% and 0.01%, respectively, in TX, and 12.6%, 3.3%, 1.3% and 0.21% in ST. No crystalline phases were detected in TX by XRD whereas ST was dominated by calcite. Cr and Fe K edge XAS showed Cr to be trivalent and octahedrally coordinated, co-precipitated with Fe as Cr x Fe 1-x -(oxy)hydroxides in both filter cakes. Zn, P and Ca K edge XAS showed that 2ZnCO 3 ∙3Zn(OH) 2 and Zn 3 (PO 4) 2 were the dominant zinc-containing phases, with combined tetrahedral and octahedral coordination; Zn phases were slightly more crystalline in TX than ST. Pb L 3 edge X-ray absorption near edge spectroscopy (XANES) found that Pb was likely adsorbed on amorphous SiO 2. Cu, Si and S K edge XAS showed that all Cu was divalent, and the dominant copper phases were found to be Cu 2 Cl(OH) 3 , Cu(OH) 2 and CuSO 4 ·5H 2 O for ST, whereas Cu appeared to adsorb to amorphous SiO 2 for TX, which contained much less Pb. Cr is thus immobilized in the filter cakes in a phase with low solubility at environmentally feasible pH values, whereas Zn, Cu and Pb could be released when the pH decreases below 8 or above 11. These findings are significant for the development of waste management regulations and/or metal recovery methods (e.g., hydro/pyrometallurgy). • Cr in filter cakes is co-precipitated with Fe as (Cr 1-x Fe x) 2 O 3 ∙ yH 2 O. • Zn is mainly 52–55% 2ZnCO 3 ∙3Zn(OH) 2 and 45–48% Zn 3 (PO 4) 2 for filter cake TX and ST at pH 8.4-8.9 • Pb and Cu are adsorbed on amorphous silica in filter cakes. • 49% Cu 2 Cl(OH) 3 , 35% Cu(OH) 2 and 16% CuSO 4 ·5H 2 O precipitate after sorption is exceeded. • Cr mobility in filter cakes is sensitive to E H ; Cu and Pb mobility is sensitive to pH. [ABSTRACT FROM AUTHOR]

Historical reconstruction of heavy metals (HMs) contamination in sediments is a key for understanding the effects of anthropogenic stresses on water bodies and predicting the variation trends of environmental state. In this work, eighteen sediment cores from the Pearl River Estuary (PRE) were collected to determine concentrations and geochemical fractions of HMs. Then, their potential sources and the relative contributions during different time periods were quantitatively identified by integrating lead-210 (210Pb) radioisotope dating technique into positive matrix factorisation (PMF) method. Pollution levels and potential ecological risks (PERs) caused by HMs were accurately assessed by enrichment factors (EF) based on establishment of their geochemical baselines (GCBs) and multiparameter evaluation index (MPE). HMs concentrations generally showed a particle size- and organic matter-dependent distribution pattern. During the period of 1958–1978, HMs concentrations remained at low levels with agricultural activities and natural processes being identified as the predominant sources and averagely contributing >60%. Since the reform and opening-up in 1978, industrial and traffic factors become the primary anthropogenic sources of HMs (such as Cu, Zn, Cd, Pb, Cr, and Ni), averagely increasing from 22.1% to 28.1% and from 11.6% to 23.4%, respectively. Conversely, the contributions of agricultural and natural factors decreased from 37.0% to 28.5% and from 29.3% to 20.0%, respectively. Subsequently, implementation of environmental preservation policies was mainly responsible for the declining trend of HMs after 2010. Little enrichment of sediment Cu, Zn, Pb, Cr and Ni with EFs (0.15–1.43) was found in the PRE, whereas EFs of Cd (1.16–2.70) demonstrated a slight to moderate enrichment. MPE indices of Cu (50.7–252), Pb (52.0–147), Zn (35.5–130), Ni (19.6–71.5), Cr (14.2–68.8) and Cd (0–9.90) highlighted their potential ecological hazards due to their non-residual fractions and anthropogenic sources. [Display omitted] • Industrialization processes contributed to the increase in HMs contents before 1978. • Implementation of pollution-control measures decreased HMs loadings in recent years. • Pollution source structure of HMs had been evidently changed during the last 70 years. • Contributions of four pollution origins to sediment HMs were quantitatively estimated. • HMs enrichment in sediments posed slight to high potential risks to aquatic ecosystem. [ABSTRACT FROM AUTHOR]

Cu (II) is a toxic heavy metal commonly identified in groundwater contaminants. Bentonite-based cutoff wall is the most used method in isolating and adsorbing contaminants, while the bentonite in it easily to fail due to Cu(II) exchange. This study synthesized a novel material through the modification of calcium bentonite (CaB) utilizing sodium hexametaphosphate (SHMP) and nano zero-valent iron (NZVI). The characteristics, adsorption performance, and mechanism of the NZVI/SHMP-CaB were investigated comprehensively. The results showed that SHMP can disperse CaB and reduce flocculation, while NZVI can be further stabilized without agglomeration. The best adsorption performance of NZVI/SHMP-CaB could be obtained at the dosage of 2% SHMP and 4% NZVI. The NZVI/SHMP-CaB exhibited an outstanding removal efficiency of over 60% and 90% at a high Cu(II) concentration (pH = 6, Cu(II) = 300 mg/L) and acidic conditions (pH = 3–6, Cu(II) = 50 mg/L), respectively. The adsorption of Cu(II) by NZVI/SHMP-CaB followed a pseudo-second-order kinetic model, and fitting results from the Freundlich isothermal model suggested that the adsorption process occurred spontaneously. Besides the rapid surface adsorption on the NZVI/SHMP-CaB and ion exchange with interlayer ions in bentonite, the removal mechanism of Cu(II) also involved the chemical reduction to insoluble forms such as Cu0 and Cu 2 O. The generated FePO 4 covered the surface of the homogenized NZVI particles, enhancing the resistance of NZVI/SHMP-CaB to acidic and oxidative environments. This study indicates that NZVI/SHMP-CaB is a promising alternative material which can be used for heavy metal removal from contaminated soil and water. [Display omitted] • NZVI/SHMP-CaB, a novel material, was synthesized for the removal of heavy metals. • Adsorption performance and mechanism of NZVI/SHMP-CaB were investigated. • Optimal ratio of 2% SHMP and 4% NZVI to CaB provided best adsorption performance. • Cu(II) was removed by rapid adsorption, ion exchange, and chemical reduction. [ABSTRACT FROM AUTHOR]

Secondary alumina dross (SAD) has emerged as an alternative to bauxite in the production of flash setting admixtures (FSA), a critical admixture in shotcrete. However, the presence of hazardous components has hampered its large-scale adoption. This study conducted field tests at an FSA factory, utilizing SAD as the primary raw material, to evaluate the feasibility and environmental risks. The results confirmed that SAD can effectively replace bauxite in FSA production without compromising quality, as it closely resembled the chemical properties of bauxite. Emissions of fluorides, heavy metals, dioxins in flue gases during production met the relevant Chinese standards. The analysis of hazardous component distribution revealed that more than 50% of volatile components, such as Cl, Cd, Pb, and Zn, were directed into fly ash, exhibiting a significant internal accumulation pattern. In contrast, more than 95% of low-volatility components, including Cu, Cr, Mn, and F, were transferred to the FSA, and the introduction of CaCO 3 was confirmed to effectively immobilize F. Moreover, the leaching risk of heavy metals and fluorides in FSA applications slightly increased but remained minimal and within acceptable limits. This technology provides an environmentally sound solution for the disposal of SAD. [Display omitted] • The use of secondary aluminum ash (SAD) on an industrial scale was rarely achieved. • SAD as a substitute for bauxite do not affect flash setting (FSA) admixtures quality. • Hazardous components in SAD were removed, stabilized and transformed. • The application risk of FSA remains within acceptable limits. [ABSTRACT FROM AUTHOR]

Identifying high-risk factors (heavy metals (HMs) and pollution sources) by coupling receptor models and health risk assessment model (HRA) is a novel approach within the field of risk assessment. However, this coupled model ignores the contribution of spatial differentiation to high-risk factors, resulting in the assessment being subjective. Taking Dongting Plain (DTP) as an example, a coupling framework by jointly using the positive matrix factorization model (PMF), HRA, Monte Carlo simulation, and geo-detector was developed, aiming to identify high-risk factors in groundwater, and further explore key environmental variables influencing the spatial heterogeneity of high-risk factors. The results showed that at least 82.86 % of non-carcinogenic risks and 97.41 % of carcinogenic risks were unacceptable for people of all ages, especially infants and children. According to the relationships among HMs, pollution sources, and health risks, As and natural sources were defined as high-risk HMs and sources, respectively. The interactions among Holocene thickness, oxidation-reduction potential, and dissolved organic carbon emerged as the primary drivers of spatial variability in high-risk factors, with their combined explanatory power reaching up to 74%. This proposed framework provides a scientific reference for future studies and a practical reference for environmental authorities in developing effective pollution management measures. • A coupling framework of source-specific risk with geo-detector was established. • Arsenic and natural sources were identified as the high-risk factors. • Geo-detector models helped interpret the PMF derived factors. • The interaction effect of factors aggravated the enrichment of heavy metals. [ABSTRACT FROM AUTHOR]

In electroplating sludge, iron (Fe) and aluminum (Al) are common impurities that need to be separated before recycling valuable heavy metals. However, the traditional Fe/Al separation process often leads to significant losses of heavy metals. To address this issue, a new approach was developed to sequentially separate Fe/Al and recycle chromium (Cr) and nickel (Ni) from real electroplating sludge. The sludge contained 4.5% Cr, 1.2% Al, 1.1% Ni, and 14.6% Fe. Initially, the sludge was completely dissolved in a mixture of hydrochloric and nitric acids. The resulting acid solution was then heated to 160 °C for 10 h with the addition of saccharose. This hydrothermal treatment led to the hydrolysis and crystallization of 98.3% of Fe, 31.8% of Cr, 1.1% of Al, and 4.9% of Ni, forming akaganeite-bearing particles. It was observed that the excessive amount of saccharose also improved the removal of Cr, Al, and Ni, but decreased the removal of Fe. After the hydrothermal treatment, the remaining supernatant was adjusted to different pH levels (1.9, 2.9, and 4.5, respectively), and then Al, Cr, and Ni were stepwise extracted using di-(2-ethylhexyl) phosphate acid (P204). The recycling efficiencies achieved were 97.4% for Al, 61.2% for Cr, and 89.3% for Ni. This approach provides a promising method for the stepwise separation of Fe/Al and the recycling of heavy metals from electroplating sludge. [Display omitted] • Electroplating sludge was recycled into akaganeite and Al/Cr/Ni crystals. • Hydrothermal treatment removed Fe/Cr with minimal Al/Ni loss compared to pH adjustment. • Approximately 98% Fe and 32% Cr were removed as akaganeite-bearing aggregates. • Sequential extraction of Al, Cr, and Ni was performed by using P204. [ABSTRACT FROM AUTHOR]

Pyrolysis of residues enriched with carbon, such as in agroforestry or industrial activities, has been postulated as an emerging technology to promote the production of biofuels, contributing to the circular economy and minimizing waste. However, during the pyrolysis processes a solid fraction residue is generated. This work aims to study the viability of these chars to develop porous carbonaceous materials that can be used for environmental applications. Diverse chars discharged by an industrial pyrolysis factory have been activated with KOH. Concretely, the char residues came from the pyrolysis of olive stone, pine, and acacia splinters, spent residues fuel, and cellulose artificial casings. The changes in the textural, structural, and composition characteristics after the activation process were studied by N 2 adsorption-desorption isotherms, scanning electron microscopy, FTIR, elemental analysis, and XPS. A great porosity was developed, S BET within 776–1186 m2 g−1 and pore volume of 0.37–0.59 cm3 g−1 with 70–90% of micropores contribution. The activated chars were used for the adsorption of CO 2 , leading to CO 2 maximum uptakes of 90–130 mg g−1. There was a good correlation between the CO 2 uptake with microporosity and oxygenated surface groups of the activated chars. Moreover, their ability to adsorption of contaminants in aqueous solution was also evaluated. Concretely, there was studied the adsorption of aqueous heavy metals, i.e., Cd, Cu, Ni, Pb, and Zn, and organic pollutants of emerging concern such as caffeine, diclofenac, and acetaminophen. [Display omitted] [ABSTRACT FROM AUTHOR]

Increasing amounts of solid waste and sludge have created many environmental management problems. Pyrolysis can effectively reduce the volume of solid waste and sludge, but there is still the problem of heavy metal contamination, which limits the application of pyrolysis in environmental management. The intercalated-exfoliated modified vermiculite (IEMV) by intercalators of sodium dodecylbenzene sulfonate, hexadecyltrimethylammonium bromide and octadecyltrimethylammonium bromide were used to control the release of Cd, Cr, Cu, Zn and Pb during pyrolysis process of sludge or solid waste. The retention of heavy metals in sludge was generally better than that in solid waste. The IEMV by octadecyltrimethylammonium bromide as the intercalator calcined 800 °C (STAB-800) was the best additive for heavy metal retention, and the retention of Cr, Cu and Zn was significantly better than that of Pb and Cd. Cr, Cu, Zn and Pb were at low risk, while Cd had considerable risk under certain circumstances. New models were proposed to comprehensively evaluate the results of the risk and forms of heavy metals, and the increasing temperature was beneficial in reducing the hazards of heavy metals by the addition of STAB-800. The reaction mechanism of heavy metals with vermiculite was revealed by simulation of reaction sites, Fukui Function and Frontier Molecular Orbital. Thermal activation-intercalated-exfoliated modified vermiculite (T-IEMV) is more reactive and had more active sites for heavy metals. Mg atoms and outermost O atoms are the main atoms for T-IEMV to react with heavy metals. The Cr, Cu and Zn have better adsorption capacity by T-IEMV than Pb and Cd. This study provides a new insight into managing solid waste and sludge and controlling heavy metal environmental pollution. [Display omitted] • Vermiculite can stabilize five heavy metals during pyrolysis of four raw materials. • New models are proposed to comprehensively evaluate the hazards of heavy metals. • Intercalation-exfoliation and calcination improve the reactivity of vermiculite. • Mg and the outermost O are main atoms of vermiculite to react with heavy metals. • Vermiculite has better adsorption capacity for Cr, Cu and Zn than for Pb and Cd. [ABSTRACT FROM AUTHOR]

Electrochemical technology is a promising technique for separating ammonia from mature landfill leachate. However, the accompanying migration and transformation of coexisting pollutants and strategies for further high-value resourceful utilization of ammonia have rarely received attention. In this study, an electrochemical separation- Rhodopseudomonas palustris electrolysis cell coupled system was initially constructed for efficient separation and conversion of nitrogen in mature landfill leachate to microbial protein with synchronously tracking the transport and conversion of coexisting heavy metals accompanying the process. The results revealed that ammonia concentration in the cathode increased from 40.3 to 49.8% with increasing the current density from 20 to 40 mA/cm2, with less than 3% of ammonia transformation to NO 2 −-N and NO 3 −-N. During ammonia separation, approximately 95% of HM-DOMs (Cr, Cu, Ni, Pb, and Zn) were released into the anolyte due to humus degradation and further diffused to the cathode. A significant correlation was observed between the releases of HM-DOMs. Cu-DOMs accounted for 70.2% of the total Cu content, which was the highest proportion among the heavy metals (HMs). Among the HMs in anolyte, 57.4% of Pb, 52.5% of Ni, and 50.6% of Zn diffused to the cathode, and most of the HMs were removed in the form of hydroxide precipitations due to heavy alkaline catholyte. Compared with the open-circuit condition, the utilization efficiency of NH 4 +-N in the R. palustris electrolysis cell increased by 445.1% with 47% and 50% increases in final NH 4 +-N conversion rate and R. palustris biomass, respectively, due to bio-electrochemical enhanced phototrophic metabolism and acid generation for buffering the strong alkalinity of the electrolyte to maintain suitable growth conditions for R. palustris. [Display omitted] • Electrochemical separation-R. palustris electrolysis cell-coupled system is designed. • Both NH 4 +-N recovery and HMs transformation in landfill leachate was concerned. • HM releases from HM-DOMs show greater correlation for degradation of HA than FA. • Most HMs removed as precipitates and met the China's discharge standard other than Pb. • Microcurrent enhanced ammonia assimilation by R. palustris and buffered catholyte. [ABSTRACT FROM AUTHOR]

Chicken manure (CM) can pose a serious threat to environmental and human health, and need to be managed properly. The compost can effectively treat CM. However, there is limited research on the heavy metals and antibiotic resistance genes (ARGs) during compost CM. In this study, the combined application of reactor and static composting (RSC) was used to produce organic fertilizer of CM (OCM), and heavy metals, ARGs and bacterial community structure was investigated. The results show that RSC could be used to produce OCM, and OCM meet the National organic fertilizer standard (NY/T525-2021). Compared to the initial CM, DTPA-Cu, DTPA-Zn, DTPA-Pb, DTPA-Cr, DTPA-Ni and DTPA-As in OCM decreased by 40.83%, 23.73%, 34.27%, 38.62%, 16.26%, and 43.35%, respectively. RSC decreased the relative abundance of ARGs in CM by 84.06%, while the relative abundance of sul1 and ermC increased. In addition, the relative abundance and diversity of ARGs were mainly influenced by the bacterial community, with Actinobacteria , Firmicutes , and Proteobacteria becoming the dominant phyla during composting, and probably being the main carriers and dispersers of most of the ARGs. Network analyses confirmed that Gracilibacillus , Lactobacillus , Nocardiopsis , Mesorhizobium and Salinicoccus were the main potential hosts of ARGs, with the main potential hosts of sul1 and ermC being Mesorhizobium and Salinicoccus. The passivation and physicochemical properties of heavy metals contribute to the removal of ARGs, with sul1 and ermC being affected by the toal heavy metals. Application of RSC allows CM to produce mature, safe organic fertilizer after 32 d and reduces the risk of rebound from ARGs, but the issues of sul1 and ermC gene removal cannot be ignored. • Composting reduces proportion of biologically effective utilization of heavy metals. • The removal of total ARGs during composting was 84.06%. • RSC reduces the risk of ARGs rebounding. • Sul1 and ermC are the main ARGs to be controlled. [ABSTRACT FROM AUTHOR]

The burgeoning interest in resource recovery from old dumpsites has significantly propelled the adoption of Landfill Mining (LFM) in recent years. This study is centred around evaluating the quality of materials recovered from the full-scale LFM activities at two major dumpsites in India, focusing on the valorization potential of the segregated legacy waste. A detailed analysis was conducted on the segregated waste fractions based on particle size (−30 mm, 30 to 6 mm, and −6 mm, as sourced from the sites), employing both batch and column leaching methods across a range of liquid-to-solid (L/S) ratios (0.1–10.0 L/kg). The findings reveal a pronounced concentration of contaminants within the −6 mm fraction compared to the 30 to 6 mm and −30 mm fractions. Column leaching tests revealed a reduction in contaminant concentration, correlating with incremental changes in L/S ratio. Notably, this trend remained consistent across varying particle sizes and specific type of contaminants assessed. Notably, color intensity of leachate reduced significantly from 720 to 1640 Platinum Cobalt Units (PCU) at an L/S ratio of 0.1 L/kg to a minimal 94–225 PCU at an L/S of 10 L/kg. Dissolved salts emerged as a primary concern, marking them as significant contaminants in both leaching methods. The analysis confirmed that the segregated fractions comply with the USEPA Waste Acceptance Criteria (WAC), permitting their disposal in non-hazardous waste landfills. However, the elevated presence of dissolved salts, exceeding reuse limits by 5–35 times, limits their open or unrestricted reuse. Despite this, isolated reuse aligns with regulations from the Netherlands and Germany, suggesting viable pathways for compliant utilization. Geotechnical assessments indicate the potential for repurposing the −30 mm fraction as alternative earthfill and construction material. While heavy metal leaching does not pose significant concerns, the prevalent unscientific disposal practices near urban settlements highlight potential human health risks. This investigation enriches the understanding of the physicochemical properties, leaching behaviour, and reuse potential of segregated legacy waste, offering crucial insights for civic authorities in determining appropriate reuse and disposal strategies for such materials. [Display omitted] • Elevated organic content, undesirable color, and dissolved salts are the contaminants of concern. • Leachable contaminants are more concentrated in FF (−6 mm) than the CF (30–6 mm). • Unrestricted reuse is not possible without reducing the contaminants of concern below threshold limits. • Isolated reuse is possible with provision of low permeable barriers around the material. • Presence of heavy metals has no significant impact on the environment and human health. [ABSTRACT FROM AUTHOR]

Acid mine drainage (AMD) caused environmental risks from heavy metal pollution, requiring treatment methods such as chemical precipitation and biological treatment. Monitoring and adapting treatment processes was crucial for success, but cost-effective pollution monitoring methods were lacking. Using bioindicators measured through 16S rRNA was a promising method to assess environmental pollution. This study evaluated the effects of AMD on ecological health using the ecological risk index (RI) and the Risk Assessment Code (RAC) indices. Additionally, we also examined how acidic metal stress affected the diversity of bacteria and fungi, as well as their networks. Bioindicators were identified using linear discriminant analysis effect size (LEfSe), Partial least squares regression (PLS-R), and Spearman analyses. The study found that Cd, Cu, Pb, and As pose potential ecological risks in that order. Fungal diversity decreased by 44.88% in AMD-affected areas, more than the 33.61% decrease in bacterial diversity. Microbial diversity was positively correlated with pH (r = 0.88, p = 0.04) and negatively correlated with bioavailable metal concentrations (r = −0.59, p = 0.05). Similarly, microbial diversity was negatively correlated with bioavailable metal concentrations (bio_Cu, bio_Pb, bio_Cd) (r = 0.79, p = 0.03). Acidiferrobacter and Thermoplasmataceae were prevalent in acidic metal environments, while Puia and Chitinophagaceae were identified as biomarker species in the control area (LDA>4). Acidiferrobacter and Thermoplasmataceae were found to be pH-tolerant bioindicators with high reliability (r = 1, P < 0.05, BW > 0.1) through PLS-R and Spearman analysis. Conversely, Puia and Chitinophagaceae were pH-sensitive bioindicators, while Teratosphaeriaceae was a potential bioindicator for Cu–Zn–Cd metal pollution. This study identified bioindicator species for acid and metal pollution in AMD habitats. This study outlined the focus of biological monitoring in AMD acidic stress environments, including extreme pH, heavy metal pollutants, and indicator species. It also provided essential information for heavy metal bioremediation, such as the role of omics and the effects of organic matter on metal bioavailability. [Display omitted] • The order of ecological risk for sources of AMD pollution had been determined to be Cd > Cu > Pb > As. • Three acid indicator species and one bioindicator species for heavy metals were screened. • A tool was provided to assess soil metal contamination and a screening model for bioindicators. [ABSTRACT FROM AUTHOR]

The escalating environmental concerns arising from soils contamination with heavy metals (HMs) and pesticides (PSTs) necessitate the development of sustainable and effective remediation strategies. These contaminants, known for their carcinogenic properties and toxicity even at small amounts, pose significant threats to both environmental ecology and human health. While various chemical and physical treatments are employed globally, their acceptance is often hindered by prolonged remediation times, high costs, and inefficacy in areas with exceptionally high pollutant concentrations. A promising emerging trend in addressing this issue is the utilization of microalgae for bioremediation. Bioremediation, particularly through microalgae, presents numerous benefits such as high efficiency, low cost, easy accessibility and an eco-friendly nature. This approach has gained widespread use in remediating HM and PST pollution, especially in large areas. This comprehensive review systematically explores the bioremediation potential of microalgae, shedding light on their application in mitigating soil pollutants. The paper summarizes the mechanisms by which microalgae remediate HMs and PSTs and considers various factors influencing the process, such as pH, temperature, pollutant concentration, co-existing pollutants, time of exposure, nutrient availability, and light intensity. Additionally, the review delves into the response and tolerance of various microalgae strains to these contaminants, along with their bioaccumulation capabilities. Challenges and future prospects in the microalgal bioremediation of pollutants are also discussed. Overall, the aim is to offer valuable insights to facilitate the future development of commercially viable and efficient microalgae-based solutions for pollutant bioremediation. [Display omitted] • Bioremediation applications of contaminated soils with HMs and PSTs were reviewed. • Uttar Pradesh and Rajasthan exhibit elevated PST usage and HM contamination. • Factors influencing bioremediation of HMs and PSTs were identified. • Mechanisms and evaluation methods of bioremediation on polluted soil were examined. • Further research is needed to advance field applications. [ABSTRACT FROM AUTHOR]

Lignin hydrothermal silica–carbon material served as a backbone for MgCl 2 activation to prepare lignin–based silicon/magnesia biochar (ALB/Si–Mg) for Cd2+, Pb2+, Cu2+, and Zn2+ removal from water and soil environment. Characterization studies revealed a 1017.71-fold increase in the specific surface area of ALB/Si–Mg compared to the original lignin biochar (ALB), producing abundant oxygen functional groups (O C–O, Si–O, Mg–O), and mineral matter (Mg 2 SiO 4 and MgO). Crucially, batch adsorption experiments demonstrated that the adsorption capacity of ALB/Si–Mg for Cd2+, Pb2+, Cu2+, and Zn2+ was 848.17, 665.07, 151.84, and 245.78 mg/g, which were 29.09–140.45 times of the ALB. Soil remediation experiments showed that applying ALB/Si–Mg increased soil effective silicon (109.04%–450.2%) and soil exchangeable magnesium (276.41%–878.66%), enhanced plant photosynthesis, and notably reduced the bioavailability of heavy metals in soil as well as the content of heavy metals in Pakchoi, thereby promoting Pakchoi growth and development. The presence of oxygen-containing functional groups on ALB/Si–Mg, along with Mg 2 SiO 4 and MgO nanoparticles, enhanced the adsorption capacity for heavy metals through the promotion of heavy metal precipitation, ion exchange, and complexation mechanisms. This study establishes the groundwork for the coupling of silica and magnesium elements in biochar and the remediation of composite heavy metal environmental pollution. • Oxygen functional groups (O C–O, Si–O, Mg–O) were produced after modification. • The coupling of silica-magnesium in biochar produced inorganic minerals (Mg 2 SiO 4). • ALB/Si–Mg effectively remediated environmental composite heavy metal pollution. • ALB/Si–Mg improved soil available silicon and exchangeable magnesium. • ALB/Si–Mg promoted growth and reduced heavy metals (Cd, Pb, Cu, Zn) in Pakchoi. [ABSTRACT FROM AUTHOR]

Prolonged wastewater irrigation in agriculture has led to the accumulation of heavy metals in soil, endangering both the soil quality and food safety, thereby posing a potential threat to human health through the consumption of contaminated crops. The present study aimed to enhance the yield of mustard (Brassica juncea L. cv. Varuna and NRCHB 101) plants and stabilize heavy metals (Cd, Cr, Ni, Cu, and Zn) in wastewater-irrigated soil using rice husk ash (RHA), rice mill by-product, collected from Chandauli region of Eastern Uttar Pradesh, India. Results demonstrated significant improvements in growth, biomass, physiology, and yield of mustard plant with increasing RHA application in wastewater irrigated soil (p ≤ 0.05). Heavy metal accumulation in different parts of mustard plants decreased as RHA application rate increased. Applying RHA at 2% in soil proved to be most effective in reducing Cd, Cr, Ni, Cu, and Zn accumulation in seeds by 29%, 29.6%, 23.1%, 21.3% and 20.1%, respectively in Varuna and 30.1%, 21.4%, 11.1%, 12.1%, and 28.5%, respectively in NRCHB 101cultivars. The present findings showed that RHA amendment in wastewater irrigated soil had reduced bioaccumulation of Cd, Cr, Ni, Cu, and Zn and consequently their toxicity in cultivated mustard plants. A novel application of RHA is unveiled in this research, offering a promising solution to promote sustainable agriculture and to reduce heavy metal associated health risks within the soil-mustard system. [Display omitted] • Effectiveness of rice husk ash amendment in soil-mustard system was investigated. • Rice husk ash application improved mustard growth in contaminated soil. • Soil pH and organic matter had negative relationships with heavy metals in seeds. • Use of Rice husk ash at the rate of 2% effectively reduced heavy metal in seeds. • Heavy metal contaminated soil can be remedied by using rice husk ash. [ABSTRACT FROM AUTHOR]

Lateritic soils are prevalent in the tropical regions, and they are used for various construction purposes including landfill liner applications. However, their contaminant attenuation potentials through sorption and the influence of parent rocks on this property are poorly understood. This study investigates lateritic soils from southwestern Nigeria as barrier to leachate migration in engineered landfills and related waste containment facilities. The lateritic soils were investigated through X-ray diffraction (XRD), geochemical analysis and batch equilibrium sorption test to evaluate the competitive sorption of Mn, Cd, Pb, Cu and Cr which are common in landfill leachates. The XRD analysis shows that the kaolinite and dickite are the dominant clay minerals present in the lateritic soils, implying low desiccation cracking and low shrink-swell behaviour. The geochemical analysis indicate that the lateritic soils are silico-alumino-feruginuous with average major oxide composition of SiO 2, Al 2 O 3 and Fe 2 O 3 of 50.86 wt%, 29.83 wt% and 14.29 wt%), respectively. Additional oxides with lower abundance include TiO 2 (1.55 wt%), Na 2 O (0.01 wt%), MgO (0.36 wt%), CaO (0.15 wt%) and K 2 O (1.52 wt%). The lateritic soils contain trace amount of heavy metals with average concentrations of Cd (0.039 ppm), Pb (0.548 ppm), Cr (0.189 ppm), Cu (0.964 ppm), Mn (0.145 ppm). Furthermore, the low abundance of sodium oxide in the lateritic soils indicates that the soil particles are not susceptible to dispersion while the presence of considerable amount of iron and manganese oxides signify its good heavy metal retention. The batch equilibrium sorption analysis shows that the lateritic soils derived from granite-gneiss and charnockite exhibit better sorption potential than those derived from schist and quartzite. This high sorption capacity is intricately related to the presence of goethite in the soils. The sorption of these trace metals onto the lateritic soils follows Langmuir type isotherm and these isotherms deviate from the corresponding desorption isotherms to different degrees indicating various extents of hysteresis. The sorption hysteresis indices for these trace metals range from 0.63 to 0.99 and imply that the trace metals may re-leached to the surrounding soils and groundwater. Thus, it is recommended that landfill liners utilizing these lateritic soils are design as a composite containment facility by integrating compacted soil liners, leachate collection systems and monitoring networks to ensure effective environmental protection. • Kaolinite and dickite dominate the lateritic soils. • The lateritic soils exhibited a pronounced tendency for the sorption of Pb and Cd. • Soils derived from granite-gneiss and charnockite showed better sorption than schist and quartzite. • Sorption isotherms exhibit varying degrees of deviation from desorption isotherms. • Hysteresis index indicates significant desorption potential. [ABSTRACT FROM AUTHOR]

Plenty of heavy metals (HMs) that are adsorbed on clay minerals (such as kaolinite), in addition to low molecular-weight organic acids (such as oxalic acid (OA)) with high activities, are widespread in the natural environment. In the present study, the effects of OA on the environmental behaviors of Pb2+/Cd2+ adsorbed by kaolinite have been investigated. The effectiveness and mechanisms of calcium silicate (CS) and magnesium silicate (MS) in reducing the environmental risks of the HMs have also been studied. The results showed that the releases of Pb2+/Cd2+ increased with an increasing concentration of OA. When different dosages of CS/MS were added to the aging system, a redistribution of HMs took place and the free form of Pb2+/Cd2+ decreased to very low levels. Also, the unextractable Pb2+/Cd2+ increased to high levels. Furthermore, a series of characterizations showed that the released HMs were re-captured by the CS/MS. In addition, the CS immobilized the OA in the solution during the aging process, which also facilitated an immobilization of the carbon element in the environment. In general, the present study has contributed to a further understanding of the transport behaviors of the HMs in natural environments, and of the interactions between CS (or MS), the environmental media, and the heavy metal contaminants. In addition, this study has also provided an eco-friendly strategy for an effective remediation of heavy metal pollution. [Display omitted] • Oxalic acid promoted the release of heavy metals fixed by kaolinite. • The silicate materials can effectively inhibit the migration of heavy metals. • The silicate materials can obviously change the form distribution of heavy metals. • The release of all metal cations and Si element during the aging process was studied. [ABSTRACT FROM AUTHOR]

The global concern regarding the adverse effects of heavy metal pollution in soil has grown significantly. Accurate prediction of heavy metal content in soil is crucial for environmental protection. This study proposes an inversion analysis method for heavy metals (As, Cd, Cr, Cu, Ni, Pb) in soil based on hyperspectral and machine learning algorithms for 21 soil reference materials from multiple provinces in China. On this basis, an integrated learning model called Stacked RF (the base model is XGBoost, LightGBM, CatBoost, and the meta-model is RF) was established to perform soil heavy metal inversion. Specifically, three popular algorithms were initially employed to preprocess the spectral data, then Random Forest (RF) was used to select the best feature bands to reduce the impact of noise, finally Stacking and four basic machine learning algorithms were used to establish comparisons and analysis of inversion model. Compared with traditional machine learning methods, the stacking model showcases enhanced stability and superior accuracy. Research results indicate that machine learning algorithms, especially ensemble learning models, have better inversion effects on heavy metals in soil. Overall, the MF-RF-Stacking model performed best in the inversion of the six heavy metals. The research results will provide a new perspective on the ensemble learning model method for soil heavy metal content inversion using data of hyperspectral characteristic bands collected from soil reference materials. [Display omitted] • Vis-NIR hyperspectroscopy is used to achieve the inversion of heavy metal content in soil. • Median filtering effectively preprocesses data and improves the accuracy of model. • Using hyperspectral characteristic bands related to HM concentrations can improve the inversion accuracy. • A fast and non-destructive MF-RF-Stacking model is developed for prediction of HM content in soil. [ABSTRACT FROM AUTHOR]

This study aims to evaluate the feasibility and safety of using municipal solid waste incineration fly ash (MSW-IFA) in the development of geopolymer-based solidification/stabilization (S/S) treatments. Geopolymers have garnered attention as a sustainable alternative to traditional cement, owing to their high strength, stability, and minimal CO 2 emissions. In this study, a combination of experimental and simulation calculations was used to investigate the setting time, mechanical properties, environmental risks, hydration mechanisms and processes of municipal solid waste incineration fly ash-based polymeric functional cementitious materials (GFCM). The results demonstrate that the mechanical properties of GFCM are related to the changes in the mineral phases and the degree of compactness. Quantum chemical calculations indicate that the hydration products may be [Si(OH) 4 ], [Al(OH) 3 (OH 2)] and [Al(OH) 4 ]-. It is possible that the heavy metals are embedded in the hydrated silica-aluminate by electrostatic interaction or chemisorption. Heavy metals may be embedded in hydrated silica-aluminate by electrostatic action or chemisorption. This study provides a feasible method for resource utilization and heavy metal stabilization mechanism of MSW-IFA. • Municipal solid waste incineration fly ash is used to synthesize geopolymer functional cementitious materials. • Geopolymer functional cementitious materials significantly immobilize heavy metals. • Geopolymer functional cementitious material is a kind of green material. • The hydration products of geopolymer functional cementitious materials investigated by density functional theory. • Heavy metals are adsorbed into silica-aluminate complexes in geopolymer materials. [ABSTRACT FROM AUTHOR]

Brick kiln co-treatment is a novel industrial hazardous wastes (IHWs) utilization process. However, the effects of chlorine (Cl) in wastes on heavy metals (HMs) during this process are overlooked. This study investigated the stabilization/solidification (S/S) and volatilization, as well as long and short-term leaching, of HMs in Cl-containing bricks. The results indicated enhanced formations of stable mineral phases (NiFe 2 O 4 , Ni 2 SiO 4 , Cd 3 Al 2 Si 3 O 12 , CdSiO 3 , FeCr 2 O 4 , Cr 2 O 3 , CuFe 2 O 4 , and CuAl 2 O 4) in bricks at a low sintering temperature (800 °C) due to the affinity between Cl and HMs. By comparing HM concentrations before and after sintering in bricks, the study observed that Cl's presence significantly elevated the volatilization rates for Cd and Cu by 30.8% and 14.2%, respectively. In contrast, the effect on volatilization for Ni and Cr was not significant. Additionally, utilizing the NEN 7375 method, the cumulative leaching rates of Ni, Cd, Cr, and Cu over a 64-day experiment under extremely acidic conditions were 0.22%, 7.18%, 0.01%, and 1.46%, respectively. Similarly, higher short-term leaching rates of Cd (4.03%) and Cu (5.73%) than those of Ni (0.94%) and Cr (0.08%) were observed. This finding might be attributed to the lower stability of the Cd and Cu solid phases under acidic environments compared to those of Ni and Cr. Surface wash-off, dissolution, and diffusion were the processes governing HM leaching from bricks. The 10-year projections revealed a minimal release of HMs during future extended leaching, implying the successful S/S of HMs. This study provides a reference for assessing the environmental impacts of brick kiln co-processing of Cl-containing IHWs. [Display omitted] • Cl promoted to the solidification/stabilization of HMs at 800 °C. • Evaluated the impact of Cl on the volatilization of HMs in bricks. • Sur-W, Dis-F, and Dif-C predominantly control HM leaching under Cl conditions. • Environmental effects of IHWs sintered brick technology have been revealed. • Brick kiln co-treatment mitigated the environmental risks of Ni and Cd. [ABSTRACT FROM AUTHOR]

Bioremediation of hazardous bauxite residues, red mud (RM), through vermicomposting has yet to be attempted. Therefore, the valorization potential of Eisenia fetida in various RM and cow dung (CD) mixtures was compared to aerobic composting. Earthworm fecundity and biomass growth were hindered in RM + CD (1:1) feedstock but enhanced in RM + CD (1:3). The pH of highly alkaline RM-feedstocks sharply reduced (>17%) due to vermicomposting. N, P, and K availability increased dramatically with Ca and Na reduction under vermicomposting. Additionally, ∼40–60% bioavailable metal fractions were transformed to obstinate (organic matter and residual bound) forms upon vermicomposting. Consequently, the total metal concentrations were significantly reduced with considerably high earthworm bioaccumulation. Microbial growth and enzyme activity were more significant under vermicomposting than composting. Correlation statistics revealed that microbial augmentation significantly facilitated a metal reduction in RM-vermibeds. Eventually, RM-vermicompost stimulated sesame growth and improved soil health with the least heavy metal contamination to soil and crop. • E. fetida fecundity was satisfactory in red mud (RM)-based vermibeds. • Alkalinity reduced by ∼17% and NPK increased by ∼30% in RM-based vermibeds. • Microbe and enzyme diversity was more in vermibeds than composting beds. • Enzyme-microbe-mediated regulation of toxic metals were promoted in vermibeds. • Vermicomposted RM induced soil-crop quality & reduced metal mobility. [ABSTRACT FROM AUTHOR]

Wastewater irrigation for vegetable cultivation is greatly concerned about the presence of toxic metals in irrigated soil and vegetables which causes possible threats to human health. This study aimed to ascertain the accumulation of heavy metals (HMs) in edible parts of vegetables irrigated with different stages of textile dyeing wastewater (TDW). Bio-concentration factor (BCF), Estimated daily intake (EDI), and target hazard quotient (THQ) were computed to estimate human health risks and speculate the hazard index (HI) of adults and children with the consumption of HMs contaminated vegetables at recommended doses. Five vegetables (red amaranth, Indian spinach, cauliflower, tomato, and radish) in a pot experiment were irrigated with groundwater (T1) and seven stages of TDW (T2∼T8) following a randomized complete block design (RCBD) with three replications. Among the TDW stages, T8, T7, T4, and T5 exhibited elevated BCF, EDI, THQ, and HI due to a rising trend in the accumulation of Pb, Cd, Cr, and Ni heavy metals in the edible portion of the red amaranth, followed by radish, Indian spinach, cauliflower, and tomato. The general patterns of heavy metal (HM) accumulation, regarded as vital nutrients for plants, were detected in the following sequence: Zn > Mn/Cu > Fe. Conversely, toxic metals were found to be Cd/Cr > Ni > Pb, regardless of the type of vegetables. Principal Component Analysis (PCA) identified T8, T7, and T4 of TDW as the primary contributors to the accumulation of heavy metals in the vegetables examined. Furthermore, the analysis of the heavy metals revealed that the BCF, THQ, and HI values for all studied metals were below 1, except for Pb. This suggests that the present consumption rates of different leafy and non-leafy vegetables, whether consumed individually or together, provide a low risk in terms of heavy metal exposure. Nevertheless, the consumption of T8, T7, and T4 irrigated vegetables, specifically Indian spinach alone or in combination with red amaranth and radish, by both adults and children, at the recommended rate, was found to pose potential health risks. On the other hand, T2, T3, and T6 irrigated vegetables were deemed safe for consumption. These findings indicated that the practice of irrigating the vegetables with T8, T7, and T4 stages of TDW has resulted in a significant buildup of heavy metals in the soils and edible parts of vegetables which are posing health risks to adults and children. Hence, it is imperative to discharge the T8, T7, and T4 stages of TDW after ETP to prevent the contamination of vegetables and mitigate potential health risks. [Display omitted] • Evaluation of metal translocation from various stages of textile dyeing wastewater. • Higher concentrations of heavy metals in wastewater lead to a greater accumulation. • The effluent was irrigated soil in the root, stem, leaf, flower, and fruit-type vegetables. • The health risk was measured by consuming heavy metals in vegetables. • Consuming contaminated leafy vegetables increases potential health hazards. [ABSTRACT FROM AUTHOR]

Enhanced phytoextraction of metal-polluted soils using EDTA is phasing out in favor of biodegradable chelants. However, these are too short-lived to be effective in the acclimated biodegrading soil environment established in long-term phytoextraction operations. We hypothesize that full-scale EDTA-enhanced phytoextraction can be both effective and environmentally safe, provided that soil leaching is prevented while EDTA persists in the soil profile. This was tested for 4 years in two sealed, well-monitored constructed lagoons (70-m3 each) packed with Cd-contaminated dredged sediment. Fast-growing, high-biomass, salinity-resistant eucalypts were planted in June 2010. Under controlled deficit irrigation, the 3-year average EC was 14.2 dS m−1. Summer leakage accounted for ∼1.2 % of the overall irrigation water and was prescribed for monitoring the composition of the soil solution. Altogether, 486 leachate and 261 suction-cap solutions were collected at average intervals of 5.5 days. EDTA was intermittently applied with summer irrigation, in multiple low doses at average seasonal concentrations of 1.1–9.2 mM. The soil solution EDTA biodegraded quickly after those applications were stopped. This cessation was timed well before the start of the rainy season. Spontaneous EDTA leaching during the three winters accounted for <0.02 % of the total 423 mol/basin applied. Prescribed summer leaching constituted ∼1 % of this total. Peak heavy metal (HM) concentrations in the leachate and suction-cap solutions (e.g., Cd, up to 18.5 and 14 mg L−1, respectively) were observed soon after EDTA application. Winter HM concentrations were not significantly different from the background. As the amounts of EDTA diminished, HM also disappeared from the soil solution, probably by adsorption. Eucalyptus occidentalis was by far the most efficient Cd sink of the five species tested,. The results of this study strongly support our hypothesis that EDTA-enhanced phytoextraction can be both effective and environmentally safe. • We tested phytostabilization/phytoextraction with EDTA for 4 years in two 72-m3 basins. • EDTA was applied with weekly irrigation in multiple low doses of up to ∼9 mM. • Controlled deficit irrigation of eucalypts arrested leaching of EDTA-metal complexes. • EDTA biodegraded, leaving ∼0.02 % of the total 443 mol applied for winter leaching. [ABSTRACT FROM AUTHOR]

An overexploitation of earth resources results in acid deposition in soil, which adversely impacts soil ecosystems and biodiversity and affects conventional heavy metal remediation using immobilization. A series of column experiments was conducted in this study to compare the cadmium (Cd) retention stability through biotic and abiotic carbonate precipitation impacted by simulated acid rain (SAR), to build a comprehensive understanding of cadmium speciation and distribution along soil depth and to elucidate the biogeochemical bacteria-soil-heavy metal interfaces. The strain of Sporosarcina pasteurii DSM 33 was used to trigger the biotic carbonate precipitation and cultivated throughout the 60-day column incubation. Results of soil pH, electrical conductivity (EC), and quantitative CdCO 3 /CaCO 3 analysis concluded that the combination of biotic and abiotic soil treatment could reinforce soil buffering capacity as a strong defense mechanism against acid rain disturbance. Up to 1.8 ± 0.04 U/mg urease enzyme activity was observed in combination soil from day 10, confirming the sustained effect of urease-mediated microbial carbonate precipitation. Cadmium speciation and distribution analyses provided new insights into the dual stimulation of carbonate-bound and Fe/Mn-bound phases of cadmium immobilization under microbially induced carbonate precipitation (MICP). As confirmed by the microbial community analysis, outsourcing urea triggered diverse microbial metabolic responses, notably carbonate precipitation and dissimilatory iron metabolism, in both oxygen-rich topsoil and oxygen-depleted subsurface layers. The overall investigation suggests the feasibility of applying MICP for soil Cd remediation under harsh environments and stratagem by selecting microbial functionality to overcome environmental challenges. [Display omitted] • A series column experiment was conducted to compare the Cd retention stability in soil. • Biotic and abiotic carbonate precipitation impacted by simulated acid rain (SAR) was studied. • The combined precipitation treatment could reinforce soil buffering capacity against acid rain. • Dual stimulation of both carbonate-bound and Fe/Mn-bound phases of cadmium immobilization was observed. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) have been widely reported to pose an adverse effect on anaerobic ammonia oxidation (anammox) bacteria, yet the underlying mechanisms remain unclear. This study provides new insights into the potential mechanisms of interaction between HMs and functional enzymes through big date analysis, molecular docking and molecular dynamics simulation. The statistical analysis indicated that 10 mg/L Cu(II) and Cd(II) reduced nitrogen removal rate (NRR) by 85% and 43%, while 5 mg/L Fe(II) enhanced NRR by 29%. Additionally, the results of molecular simulations provided a microscopic interpretation for these macroscopic data. Molecular docking revealed that Hg(II) formed a distinctive binding site on ferritin, while other HMs resided at iron oxidation sites. Furthermore, HMs exhibited distinct binding sites on hydrazine dehydrogenase. Concurrently, the molecular dynamics simulation results further substantiated their capacity to form complexes. Cu(II) displayed the strongest binding affinity with ferritin for −1576 ± 79 kJ/mol in binding free energy calculation. Moreover, Cd(II) bound to ferritin and HDH for −1052.67 ± 58.49 kJ/mol, −290.02 ± 49.68 kJ/mol, respectively. This research addressed a crucial knowledge gap, shedding light on potential applications for remediating heavy metal-laden industrial wastewater. [Display omitted] • Cu(II) and Cd (II) had the strongest inhibitory effect on NRR. • The binding sites of heavy metals on enzymes were predicted by molecular docking. • Heavy metals did not significantly impact the structure of enzymes. • Ferritin was bound with all metals, especially Cu(II) with the highest BFR. • Cd(II) bound to both HDH and ferritin, with a preference for ferritin. [ABSTRACT FROM AUTHOR]

Protecting the environment from harmful pollutants has become increasingly difficult in recent decades. The presence of heavy metal (HM) pollution poses a serious environmental hazard that requires intricate attention on a worldwide scale. Even at low concentrations, HMs have the potential to induce deleterious health effects in both humans and other living organisms. Therefore, various strategies have been proposed to address this issue, with extremophiles being a promising solution. Bacteria that exhibit resistance to metals are preferred for applications involving metal removal due to their capacity for rapid multiplication and growth. Extremophiles are a special group of microorganisms that are capable of surviving under extreme conditions such as extreme temperatures, pH levels, and high salt concentrations where other organisms cannot. Due to their unique enzymes and adaptive capabilities, extremophiles are well suited as catalysts for environmental biotechnology applications, including the bioremediation of HMs through various strategies. The mechanisms of resistance to HMs by extremophilic bacteria encompass: (i) metal exclusion by permeability barrier; (ii) extracellular metal sequestration by protein/chelator binding; (iii) intracellular sequestration of the metal by protein/chelator binding; (iv) enzymatic detoxification of a metal to a less toxic form; (v) active transport of HMs; (vi) passive tolerance; (vii) reduced metal sensitivity of cellular targets to metal ions; and (viii) morphological change of cells. This review provides comprehensive information on extremophilic bacteria and their potential roles for bioremediation, particularly in environments contaminated with HMs, which pose a threat due to their stability and persistence. Genetic engineering of extremophilic bacteria in stressed environments could help in the bioremediation of contaminated sites. Due to their unique characteristics, these organisms and their enzymes are expected to bridge the gap between biological and chemical industrial processes. However, the structure and biochemical properties of extremophilic bacteria, along with any possible long-term effects of their applications, need to be investigated further. • Extremophiles provide a promising approach for the removal of heavy metals (HMs). • Extremophilic bacteria have unique enzymatic activities and adaptive capabilities. • Species of bacteria for the tolerance of HMs are reviewed. • Resistance mechanisms of tolerance of HMs by extremophilic bacteria are discussed. [ABSTRACT FROM AUTHOR]

Understanding the porosity of biochar (BC) that promotes the heavy metal (HM) passivation during composting can contribute to the sustainable management of pig manure (PM). The current work aimed to explore the influence of BC with varying pore sizes on the physicochemical properties and morphological changes of HMs (including Zn, Cu, Cr, As, and Hg), and microbiota development during PM composting. The various pore sizes of BC were generated by pyrolyzing pine wood at 400 (T1), 500 (T2), 600 (T3) and 700 (T4) °C, respectively. The results revealed a positive correlation between specific surface area of BC and pyrolysis temperature. BC addition contributed to a significantly extended compost warming rate and duration of high-temperature period, as well as HM passivation, reflected in the decrease in Exc-Zn (63-34%) and Red-Cu (28-13%) content, and the conversion of Oxi-Cr (29-21%) and Red-Hg (16-5%) to more stable forms. Moreover, BC at T4 exhibited the best effect on Zn and Cu passivation due to the highest specific surface area (380.03 m2/g). In addition to its impact on HM passivation, BC addition improved the microbial environment during PM composting, leading to enhanced microbial diversity and richness. Notably, Chloroflexi and Bacteroidota played key roles in promoting the transformation of Exc-Cu and Red-Hg into stable forms. This phenomenon further stimulated the enhanced decomposition of organic matter (OM) when BC prepared at 600–700 °C was added. Therefore, it can be concluded that the regulation of BC porosity is an effective strategy to improve HM passivation and the overall effectiveness of PM composting. [Display omitted] • Specific surface area of biochar positively corelate with pyrolysis temperatures. • Higher temperature prepared biochar exhibits better heavy metal passivation effect. • The addition of biochar increased Cu、Zn and Hg passivation rate by more than 46%. • Chloroflexi and Bacteroidota involve in transforming heavy metals into stable forms. [ABSTRACT FROM AUTHOR]

To determine the priority control sources, an approach was proposed to evaluate the source-specific contribution to health risks from inhaling PM 2.5 -bound heavy metals (PBHMs). A total of 482 daily PM 2.5 samples were collected from urban and suburban areas of Beijing, China, between 2018 and 2019. In addition to the PMF-PSCF model, a Pb isotopic IsoSource model was built for more reliable source apportionment. By using the comprehensive indicator of disability-adjusted life years (DALYs), carcinogenic and noncarcinogenic health risks could be compared on a unified scale. The study found that the annual average concentrations of the total PBHMs were significantly higher in suburban areas than in urban areas, with significantly higher concentrations during the heating season than during the nonheating season. Comprehensive dust accounted for the largest contribution to the concentration of PBHMs, while coal combustion contributed the most to the DALYs associated with PBHMs. These results suggest that prioritizing the control of coal combustion could effectively reduce the disease burden associated with PBHMs, leading to notable public health benefits. • Most PBHMs have higher concentrations in Beijing suburban areas than in urban areas. • The PMF-PSCF-IsoSource model can reduce the uncertainty in source apportionment. • Coal and fuel oil combustion should be controlled with a priority for public health. • The disease burden of noncarcinogenic effects must not be overlooked. [ABSTRACT FROM AUTHOR]

The most common construction material used in Taiwan is concrete, potentially contaminated by geologic heavy metals (HMs). Younger children spend much time indoors, increasing HM exposure risks from household dust owing to their behaviors. We evaluated arsenic (As), cadmium (Cd), and lead (Pb) concentrations in fingernails among 280 preschoolers between 2017 and 2023. We also analyzed HM concentrations, including As, Cd, Pb, chromium (Cr), nickel (Ni), copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn), in 90 household dust and 50 road dust samples from a residential area where children lived between 2019 and 2021 to deepen the understanding of sources and health risks of exposure to HMs from household dust. The average As, Cd, and Pb concentrations in fingernails were 0.12 ± 0.06, 0.05 ± 0.05, and 0.95 ± 0.77 μg/g, respectively. Soil parent materials, indoor construction activities, vehicle emissions, and mixed indoor combustion were the pollution sources of HMs in household dust. Higher Cr and Pb levels in household dust may pose non-carcinogenic risks to preschoolers. Addressing indoor construction and soil parent materials sources is vital for children's health. The finding of the present survey can be used for indoor environmental management to reduce the risks of HM exposure and avoid potential adverse health effects for younger children. [Display omitted] • Ni and Cr in metropolitan household dust may originate from building construction materials. • Higher heavy metal levels in household dust may pose a non-carcinogenic risk for younger children. • It's crucial to prioritize the management of Cr and Pb levels in indoor dust. • Indoor construction activity was the major contributor to non-carcinogenic risks for younger children. [ABSTRACT FROM AUTHOR]

Bioretention is an increasingly common stormwater control measure (SCM) for mitigation of stormwater quantity and quality. Studies from lab to field scale have shown successful removal of total metals from stormwater, especially Cu and Zn which are ubiquitous in the urban environment yet detrimental to aquatic ecosystems. While bioretention effectively removes particulate matter and particulate bound (PB) contaminants, removal performance of dissolved metals has been neglected in field studies. After approximately two decades of these systems being implemented, with a typical design-life of 20 years, performance of mature systems is unknown. This study examined the performance of a 16- to 18-year-old bioretention cell by characterizing Cu and Zn partitioning and removal. Flow-weighted composite samples of stormwater and bioretention effluent were collected and analyzed for total and dissolved metals. Size-fractioned road-deposited sediments (RDS) were collected and analyzed for metals and particle size distribution. The comparison of RDS and PB metals showed that PB-Zn was enriched in stormwater, indicating higher mobility of PB-Zn compared to PB-Cu. The mature bioretention system effectively removed particulates and PB-metals with average load reductions of 82% and 83%, respectively. While concentrations for dissolved metals were low (<40 μg/L), no significant difference between influent and effluent was observed. Effluent concentrations of total and dissolved Cu, total organic carbon, and particulates were not significantly different from those measured over 10 years ago at the site, while total Zn effluent concentration slightly increased. MINTEQ speciation modeling showed that Cu was approximately 100% bound with dissolved organic matter (DOM) in all bioretention effluent. While Zn was also mostly bound with DOM in effluent, some events showed free ionic Zn reaching concentrations in the same order of magnitude. Media amendments, maintenance, and monitoring of SCMs should be considered where further removal of dissolved metals is necessary for the protection of aquatic environments. [Display omitted] • First study on water quality performance of a mature bioretention cell. • Road-deposited sediments, stormwater, and bioretention effluent collected. • Metal partitioning, particle-size affiliation, and speciation modeling studied. • Bioretention was effective for removal of particulate but not dissolved phase. • Insights on impact of pollutant source and mobility in urban stormwater. [ABSTRACT FROM AUTHOR]

The cracked phosphorylated nanoscale zerovalent iron (p-nZVI) has a strong electron selectivity towards the reductive removal of many heavy metal ions in waters. However, the unintended environmental risk after interactions with impurities or wastewater are not involved. Therefore, in this study, the phosphate group was successfully adsorbed into p-nZVI, and the cracked p-nZVI was successfully prepared with an optimal P/Fe ratio of 0.5%. The dosages of p-nZVI and temperatures were positively correlated with the removal rates. The removal process of Cr(VI) was more suitable by the Langmuir isothermal model（R2 > 0.99). The process of Cr (VI) (10, 20 and 40 mg/L) removal more fitted the pseudo first-order reaction model, while the process of Cr (VI) (60, 80 mg/L) removal more fitted the pseudo second-order reaction model. The Cr (VI) removal rates gradually decreased when the pH was increased. Dissolved oxygen slowed nanoiron reaction rates. The order of inhibition on the reactivity towards Cr(VI) was SiO 3 2− > SO 4 2− > PO 4 3− > NO 3 − > HCO 3 −.The facilitation followed the order of Cd2+>Cu2+>Mg2+>Mn2+>Ca2+. Ca2+ showed an inhibitory effect, but all other cations showed different degrees of facilitation. The promotion effect is relatively similar in presence of Mn2+ or Mg2+. HA had a significant inhibitory effect. Environmental friendly p-nZVI had a good effect in simulated groundwater, seawater, river water and secondary effluent of the urban sewage treatment plant. The main pathway to remove Cr (VI) was in situ reduction by p-nZVI. The improved adsorption and reduction effect of p-nZVI on heavy metal ions in water was due to the structural change and the phosphate group. • The unique structure and phosphate are vital to outstanding utilization efficiency. • p-nZVI sustained its high efficiency in the waters with impurities. • The order of inhibition was SiO 3 2− > SO 4 2− > PO 4 3− > NO 3 − > HCO 3 −, Ca2+ >Mn2+>Mg2+>Cu2+>Cd2+. • The oxidation rate of Fe(0) was relatively slow in p-nZVI. [ABSTRACT FROM AUTHOR]

The recycling and utilization of phosphorus resources in sludge is becoming increasingly important. In this study, we compared the conversion of phosphorus and toxic metal passivation effects of different Ca additives under oxygen-rich combustion conditions and elucidated their specific mechanisms of action. The experimental results indicated that four Ca-based additives improved the recovery rate of total phosphorus, and promoted the generation of stable apatite phosphorus (AP). The effect of CaCl 2 and CaO was greater than that of Ca(OH) 2 and CaSO 4. CaCl 2 promoted the formation of Ca 3 (PO 4) 2 and Ca 2 P 2 O 7 , and CaSO 4 improved the conversion of AlPO 4 to Ca(H 2 PO 4) 2 with increasing temperature. The conversion capacity of CaO on non-apatite inorganic phosphorus to AP was greater than that of Ca(OH) 2 , and more CaH 2 P 2 O 7 , Ca(PO 3) 2 , and Ca–Al–P minerals were found. Toxic metal percentages decreased after sludge incineration with CaCl 2. Compared with CaO and Ca(OH) 2 , the toxic metal adsorption effect of CaSO 4 was more significant. The influence of Ca additives on the conversion of Zn into stable components was as follows: CaCl 2 > Ca(OH) 2 > CaO > CaSO 4. Ca additives reduced the toxic metal contamination level and ecological risk index values, and the order of toxic metal contamination levels was Ni > Zn > Cr > Cu > Mn. The experiment confirmed the conversion of phosphorus and the toxic metal passivation effect of Ca additives during oxy-fuel combustion of sludge, which is beneficial for its resource utilization. • Ca-based additives and temperature jointly affect the total amount of phosphorus. • Ca additives promote the generation of stable AP. • Heavy metal percentages decrease after sludge incineration with CaCl 2. • Ca-additives Reduce environmental risks of heavy metals. [ABSTRACT FROM AUTHOR]

Due to their use in a number of advanced electronic technologies, Rare earth elements (REEs) have recently emerged as a key strategic resource for many nations worldwide. The significant increase in demand for REEs has thus greatly increased the mining of these substances, but this industrial-scale expansion of mining activities also poses potential risks to the surrounding environment, flora, fauna, and humans. Hence efficient REE remediation is one potential remediation process involving in situ clean-up of contaminated soil which has gained much attention in recent years, due to its low cost and lack of secondary pollution. However, some crucial aspects of phytoremediation, such as the precise-mechanisms of absorption, transport, and tolerance of REEs by hyperaccumulators -are poorly understood. This review briefly discusses the environmental risks associated with excess REEs, the efficacy of phytoremediation technologies coupled with, appropriate hyperaccumulator species to migrate REEs exposure. While REEs hyperaccumulator species should ideally be large-biomass trees and shrubs suitable for cropping in subtropical regions areas, such species have not yet been found. Specifically, this review focuses on the factors affecting the bioavailability of REEs in plants, where organic acids are critical ligands promoting efficient transport and uptake. Thus the uptake, transport, and binding forms of REEs in the above-ground parts of hyperaccumulators, especially the transporters isolated from the heavy metal transporter families, are discussed in detail. Finally, having summarized the current state of research in this area, this review proceeds to discuss current knowledge gaps and research directions. With a focus on hyperaccumulators, this review serves as a basis for future phytoremediation strategies of rare earth mining-impacted environments and addresses ecosystem/environmental degradation issues resulting from such mining activity. [Display omitted] • Phytoremediation is a promising remediation technology for REEs remediation. • The REEs distribution and identified fern hyperaccumulators are given. • The key factors affecting the bioavailability of REEs are summarized. • The transporters involve in the transport process of hyperaccumulators. • This review provides a basis for scientific REE phytoremediation strategies. [ABSTRACT FROM AUTHOR]

Phytoremediation is an environmentally friendly, economical, and sustainable technique for restoring farmland. It can remove heavy metals and organic pollutants from the soil through the implementation of hyperaccumulator plants. In recent years, it has garnered significant interest from academic and industrial sectors. This article screened 368 research papers from the Web of Science core collection database related to farmland phytoremediation and conducted a bibliometric analysis of the domain based on CiteSpace. The paper intuitively demonstrates the most influential countries, the most productive institutions, the most contributing groups of authors, and the primary sources of farmland phytoremediation research domain. The findings additionally indicate that the research hotspots include: (1) mechanisms and principles of phytoremediation, (2) the improvement of restoration efficiency, (3) the economic, ecological, and sustainable development of phytoremediation. The exploration of plants with potential to accumulate heavy metals and produce large amounts of biomass is the research frontier within the field of farmland phytoremediation. Additionally, this bibliometric analysis can help scholars willing to work in this research field by concisely understanding the overall research field and frontiers. With the continuous improvement of phytoremediation and its combination with other remediation technologies, the future of farmland remediation will have a promising prospect. • Papers of farmland phytoremediation in the past three decades were comprehensively and systematically bibliometric analyzed. • Internal connection of farmland phytoremediation was elaborated from various aspects. • Research hotspots and frontiers of farmland phytoremediation were clarified through keyword cluster analysis. [ABSTRACT FROM AUTHOR]

Heavy metal and antibiotic-resistant bacteria from livestock feces are ecological and public health problems. However, the distribution and relationships of antibiotic resistance genes (ARGs), heavy metal resistance genes (HMRGs), and virulence factors (VFs) and their transmission mechanisms remain unclear. Therefore, we investigated the resistance of Escherichia coli , the prevalence of its ARGs, HMRGs, and VFs, and their transmission mechanisms in livestock fresh feces (FF), composted feces (CF), and fertilized soil (FS). In total, 99.54% (n = 221) and 91.44% (n = 203) of E. coli were resistant to at least one antibiotic and one heavy metal, respectively. Additionally, 72.52% (n = 161) were multi-drug resistant (MDR), of which Cu-resistant E. coli accounted for 72.67% (117/161). More than 99.34% (88/89) of E. coli carried multidrug ARGs, VFs, and the Cu resistance genes cueO and cusABCRFS. The Cu resistance genes cueO and cusABCRFS were mainly located on chromosomes, and cueO and cusF were positively associated with HMRGs, ARGs, and VFs. The Cu resistance genes pcoABCDRS were located on the plasmid pLKYL-P02 flanked by ARGs in PF18C from FF group and on chromosomes flanked by HMRGs in SAXZ1-1 from FS group. These results improved our understanding of bacterial multidrug and heavy metal resistance in the environment. [Display omitted] • The average Cu concentrations were most abundant in the FF group. • The majority of E. coli isolates were identified as MDR. • Cu resistance genes were positively correlated with ARGs, HMRGs, and VFs. • A novel plasmid harboring pcoABCDRS was identified. • pcoABCDRS co-occurred with ARGs on a plasmid and HMRGs on the chromosome. [ABSTRACT FROM AUTHOR]

An Acinetobacter calcoaceticus strain HM12 capable of heterotrophic nitrification-aerobic denitrification (HN-AD) under nutrient-poor conditions was isolated, with an ammonia nitrogen (NH 4 +-N) removal efficiency of 98.53%. It can also remove heavy metals by microbial induced calcium precipitation (MICP) with a Ca2+ removal efficiency of 75.91%. Optimal conditions for HN-AD and mineralization of the strain were determined by kinetic analysis (pH = 7, C/N = 2.0, Ca2+ = 70.0 mg L−1, NH 4 +-N = 5.0 mg L−1). Growth curves and nitrogen balance elucidated nitrogen degradation pathways capable of converting NH 4 +-N to gaseous nitrogen. The analysis of the bioprecipitation showed that Zn2+ and Cd2+ were removed by the MICP process through co-precipitation and adsorption (maximum removal efficiencies of 93.39% and 80.70%, respectively), mainly ZnCO 3 , CdCO 3 , ZnHPO 4 , Zn 3 (PO 4) 2 and Cd 3 (PO 4) 2. Strain HM12 produces humic and fulvic acids to counteract the toxicity of pollutants, as well as aromatic proteins to increase extracellular polymers (EPS) and promote the biomineralization process. This study provides a experimental evidence for the simultaneous removal of multiple pollutants from nutrient-poor waters. [Display omitted] • Isolation of Acinetobacter calcoaceticus strain HM12 capable of HN-AD co-MICP. • ExpDec1 model was used for kinetic analysis of strain HM12. • NH 4 +-N and Ca2+ removal efficiencies reached 98.53% and 75.91%, respectively. • Zn2+ and Cd2+ removal efficiencies reached 93.39% and 80.70%, respectively. • Strain HM12 has excellent adaptation to nutrient-poor environments. [ABSTRACT FROM AUTHOR]

Previous researches indicate that the potent toxicity of cadmium hinders the efficacy of the microbial-induced carbonate precipitation (MICP) process for bioremediation of Cd2+ in aqueous environment. Increasing urea and calcium resource doses, introducing synergists, and utilizing urease-producing consortia can improve bio-immobilization performance of MICP. However, such measures may incur cost increases and/or secondary contamination. This study first verifies the substantial biotoxicity of Cd2+ for urease activity and then analyzes the practical limitation of traditional MICP using Bacillus pasteurii for bioremediation of Cd2+ in an aqueous environment containing 1–40 mM Cd2+ by a series tube tests and numerical simulation. Subsequently, a two-step MICP method, which separates urea hydrolysis and heavy metal precipitation, is introduced in this study to eliminate the inhibitory effect of heavy metal on urease activity. The concentrations of ammonium, Cd2+, and pH were monitored over time. The results indicate that the urease expression in B. pasteurii can be significantly inhibited by Cd2+ particularly at the concentration ranging from 10 to 40 mM, leading to pretty low efficacy of traditional MICP for bioremediation of Cd2+ (Cd2+ removal rate as low as 21.55–38.47% when the initial Cd2+ concentration = 40 mM). In contrast, when the two-step MICP method is applied, the Cd2+ can be almost completely immobilized, even though the concentration ratio of urea to Cd2+ is as low as 1.5:1.0, which is close to the theory minimum concentration ratio for the complete precipitation of carbonate to cadmium ions(1.0:1.0). Therefore, the cost-effective, environmentally sustainable, and straightforward two-step MICP method holds great potential for application in the bioremediation of Cd2+-contaminated solutions in high concentration. [Display omitted] • Cd2+ has toxic effects on urease activity more severely as the initial Cd2+ concentration rises. • Cd2+ hinders the immobilization efficiency of the traditional MICP method. • The two-step MICP method holds application potential for bioremediation of Cd2+-contaminated solutions in high concentration. [ABSTRACT FROM AUTHOR]

Olive oil mill wastewater (OMW) is an environmental concern in olive oil producers' regions due to its use in agricultural soils as an organic amendment. However, OMW can also be used as organic fertilizer due to their high organic matter and nutrient levels, but its use, when it occurs without environmental management, can cause serious environmental implications for soils and waters. This work evaluated the impact of different OMW levels on a set of physicochemical parameters from an agricultural vertisol where wheat grew (Triticum aestivum L var. Douma 1). A set of physicochemical parameters were conducted before adding different levels of OMW (0, 5, 10 and 15 L m−2) at two soil depths (0–30 and 30–60 cm) and for the two growing seasons to determine: i) the effect of OMW treatments on the studied physicochemical soil properties (bulk density, soil porosity, soil pH, electrical conductivity and organic matter), ii) available primary (N, P, K) and secondary macronutrients (Ca, Mg and Na), ii) micronutrients (Cu Fe, Mn and Zn), and iv) available heavy metals (Cd and Pb). The results indicated that soil physicochemical parameters were slightly improved, mainly due to improvement in organic matter, macro- and micronutrients, usually proportionally to the olive mill wastewater dose. Cadmium and Pb were within the permissible limits. The increased OMW had different behaviour on the soil nutritional balances of different elements, leading to nutrient imbalances, although in some cases, they were improved. However, the plant growth was not affected, and it was improved under 10 L m−2 and 15 L m−2 doses. The results offer valuable data about the use of OMW as organic fertilizer for crops and their potential impact on soil properties. • Olive oil wastewater can contaminate aquatic and terrestrial ecosystems. • Olive oil wastewaters have been widely used on nutrient-poor soils as fertilizers. • Organic matter and soil nutrients were improved after wastewater application. [ABSTRACT FROM AUTHOR]

Heavy metal stabilization is an effective method to treat chromium in tannery sludge. Here we show that mainly investigated NaH 2 PO 4 (MSP) and organic matter (OM) to stabilize chromium in tannery sludge. The experimental investigation revealed that the addition of montmorillonite (MMT) and MSP samples showed a significant increase in the percentage of reducible and oxidizable Cr in the former compared to the samples with the addition of MMT. This is attributed to the formation of Cr–O bond, which allows the MSP to undergo an inner-sphere complexation reaction with the metal oxide of Cr via ligand exchange. Significantly, the MSP moiety adsorbs on the surface of OM through monodentate, which increases the adsorption sites of OM for Cr6+ and promotes the reduction of Cr6+ to Cr3+. Moreover, PO 4 3− reacts with Cr3+ to produce CrPO 4 precipitation, thus reducing the free Cr3+ content. Finally, DFT calculations confirmed that a ternary system is formed between PO 4 3−, OM, and Cr, and the binding energy is negative, which indicated that PO 4 3− could co-stabilize Cr with OM. [Display omitted] • Stabilization of Cr by MSP is achieved through ligand exchange. • MSP amplifies the adsorption sites of OM and promotes the reduction of Cr6+. • PO 4 3− from the dissociation of MSP reacts with Cr3+ to form CrPO 4 precipitate. • Results from DFT indicate the presence of PO 4 3−, OM, and Cr in a ternary system. [ABSTRACT FROM AUTHOR]

Short-term influence of polytetrafluoroethylene micro/nano-plastics (PTFE-MPs/NPs) on the inhibition of copper (Cu2+) and/or ciprofloxacin (CIP) on the nitrifying sludge activities was explored based on concentration addition (CA) and independent action (IA) models. The half maximal inhibitory concentration (IC 50) of Cu2+, CIP, PTFE-MPs (3 μm), and PTFE-NPs (800 nm) on the specific ammonium oxidation rate (SAOR) of nitrifying sludge was 64.57, 51.29, 102.33 and 93.33 mg L−1, respectively, while those on the specific nitrite oxidation rate (SNOR) of nitrifying sludge were 77.62, 32.36, 104.70 and 97.72 mg L−1, respectively. Among the five binary mixtures and two ternary mixtures composed by Cu2+, CIP, and/or PTFE-MPs/NPs, it was found that the two joint inhibitory actions from ternary mixtures on the SAOR and SNOR of the sludge showed time-dependent characteristics by analyzing of CA and IA models, while the five combined inhibitory effects from different binary mixtures did not all have time-dependent features. The two joint inhibition actions from diverse ternary mixtures on the SAOR at the exposure time of 60 min and on the SNOR at 90 min showed always concentration-dependent features, while the combined inhibitions with concentration-dependent characteristics had never been observed in the binary Cu2+ and PTFE-NPs mixtures at different exposure time. The Cu2+, CIP, and PTFE-MPs mixtures (or Cu2+, CIP, and PTFE-NPs mixtures) had synergistic actions on the SAOR at 90 min and antagonistic effects on the SNOR at 60 min based on CA and IA models, and these combined inhibitions did not exhibit concentration-dependent characteristics. In contrast, the joint inhibitory effects (on the SAOR and SNOR) with concentration-dependent features were found in the binary mixtures of CIP and PTFE-MPs at different exposure time, and the join inhibition changed from synergism to antagonism as the increasing concentration of mixed CIP and PTFE-MPs. This study provides novel perspectives for understanding the combined influence of plastic particles with different sizes, antibiotics, and heavy metals on the biological wastewater treatment process. • The order of toxicity among contaminants was CIP > Cu2+> PTFE-NPs > PTFE-MPs. • Joint CIP, Cu2+ and/or PTFE-NPs/MPs toxicity was analyzed by CA and IA models. • PTFE-NPs with CIP and/or Cu2+ mixtures exhibited a lower toxicity on SAOR. • The mixed toxicity of CIP, Cu2+ and/or PTFE-NPs/MPs depended on content and time. • Mixed CIP, Cu2+ and/or PTFE-NPs/MPs toxicity to SAOR was different from that of SNOR. [ABSTRACT FROM AUTHOR]

The combination of organic and heavy metal pollutants can be effectively and sustainably remediated using bioremediation, which is acknowledged as an environmentally friendly and economical approach. In this study, bacterial agent YH was used as the research object to explore its potential and mechanism for bioremediation of pyrene-heavy metal co-contaminated system. Under the optimal conditions (pH 7.0, temperature 35°C), it was observed that pyrene (PYR), Pb(II), and Cu(II) were effectively eliminated in liquid medium, with removal rates of 43.46%, 97.73% and 81.60%, respectively. The microscopic characterization (SEM/TEM-EDS, XPS, XRD and FTIR) results showed that Pb(II) and Cu(II) were eliminated by extracellular adsorption and intracellular accumulation of YH. Furthermore, the presence of resistance gene clusters (cop , pco , cus and pbr) plays an important role in the detoxification of Pb(II) and Cu(II) by strains YH. The degradation rate of PYR reached 72.51% in composite contaminated soil, which was 4.33 times that of the control group, suggesting that YH promoted the dissipation of pyrene. Simultaneously, the content of Cu, Pb and Cr in the form of F4 (residual state) increased by 25.17%, 6.34% and 36.88%, respectively, indicating a decrease in the bioavailability of heavy metals. Furthermore, YH reorganized the microbial community structure and enriched the abundance of hydrocarbon degradation pathways and enzyme-related functions. This study would provide an effective microbial agent and new insights for the remediation of soil and water contaminated with organic pollutants and heavy metals. [Display omitted] • The bifunctional bacterial agent YH with the ability to degrade pyrene (PYR) and resist heavy metals was utilized. • The removal efficiency of the YH for PYR, Pb(II) and Cu(II) were 43.46%, 97.73% and 81.60%. • The YH reduced the bioavailability and environmental risk of Pb, Cu and Cr in soil. • The ABC transporter, hydrocarbon degradation and heavy metal resistance enzymes were enhanced. [ABSTRACT FROM AUTHOR]

Heavy metal contamination of soil commonly accompanies problems around gold mine tailings ponds. Fully investigating the distribution characteristics of heavy metals and the survival strategies of dominant plants in contaminated soils is crucial for effective pollution management and remediation. This study aims to investigate the contamination characteristics, sources of heavy metals (As, Cd, Pb, Hg, Cu, Zn, Cr, and Ni) in soils around gold mine tailings ponds areas (JHH and WZ) and to clarify the form distribution of heavy metals (As, Cd, Pb, Hg) in contaminated plots as well as their accumulation and translocation in native dominant plants. The results of the study showed that the concentrations of As, Pb, Cd, Cu, and Zn in soil exceeded the national limits at parts of the sampling sites in both study areas. The Nemerow pollution index showed that both study areas reached extreme high pollution levels. Spatial analysis showed that the main areas of contamination were concentrated around metallurgical plants and tailings ponds, with Cd exhibiting the most extensive area of contamination. In the JHH, As (74%), Cd (66%), Pb (77%), Zn (47%) were mainly from tailings releases, and Cu (52%) and Hg (51%) were mainly from gold ore smelting. In the WZ, As (42%), Cd (41%), Pb (73%), Cu (47%), and Zn (41%) were mainly from tailings releases. As, Cd, Pb, and Hg were mostly present in the residue state, and the proportion of water-soluble, ion-exchangeable, and carbonate-bound forms of Cd (19.93%) was significantly higher than that of other heavy metals. Artemisia L. and Amaranthus L. are the primary dominating plants, which exhibited superior accumulation of Cd compared to As, Pb, and Hg, and Artemisia L. demonstrated a robust translocation capacity for As, Pb, and Hg. Compared to the concentrations of other forms of soil heavy metals, the heavy metal content in Artemisia L correlates significantly better with the total soil heavy metal concentration. These results offer additional systematic data support and a deeper theoretical foundation to bolster pollution-control and ecological remediation efforts in mining areas. [Display omitted] • Pb, As, Cd, Cu, Zn and Hg are the main heavy metal polluting elements in a typical gold mining area. • The primary sources of Pb, As, Cd, Cu, and Zn are tailings releases, while Hg originates mainly from gold smelting. • The concentration of unstable forms of Cd was significantly higher than that of other heavy metals in the mine areas. • Artemisia L. has a better enrichment capacity for Cd, but a better translocation capacity for As, Pb, and Hg. • Heavy metal concentration in Artemisia L. correlate better with those of stable forms in soil than unstable forms. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) pollution threatens food security and human health. While previous studies have evaluated source-oriented health risk assessments, a comprehensive integration of environmental capacity risk assessments with pollution source analysis to prioritize control factors for soil contamination is still lacking. Herein, we collected 837 surface soil samples from agricultural land in the Nansha District of China in 2019. We developed an improved integrated assessment model to analyze the pollution sources, health risks, and environmental capacities of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn. The model graded pollution source impact on environmental capacity risk to prioritize control measures for soil HMs. All HMs except Pb exceeded background values and were sourced primarily from natural, transportation, and industrial activities (31.26%). Approximately 98.92% (children), 97.87% (adult females), and 97.41% (adult males) of carcinogenic values exceeded the acceptable threshold of 1E-6. HM pollution was classified as medium capacity (3.41 kg/hm2) with mild risk (PI = 0.52). Mixed sources of natural backgrounds, transportation, and industrial sources were identified as priority sources, and As a priority element. These findings will help prioritize control factors for soil HMs and direct resources to the most critical pollutants and sources of contamination, particularly when resources are limited. [Display omitted] • We examined heavy metal pollution in soil samples from Nansha District, China. • We integrated pollution source, health risk, and environmental capacity analyses. • Correlation and probabilistic matrix factorization identified pollution sources. • Most examined elements showed carcinogenic values exceeding acceptable thresholds. • Natural, transport, and industry sources and arsenic are priority control factors. [ABSTRACT FROM AUTHOR]

Ten novel hydrophobic dicationic ionic liquids (DILs) were synthesized and applied for the extraction of heavy metals in aqueous solutions. Their physicochemical properties were measured at ambient temperature, and the leaching behaviors of the as-prepared DILs in water were assessed by TOC analysis. Metal extraction experiments were carried out to evaluate the extraction performances of the DILs. It was found that the extraction rates of up to 0.45 and 0.53 mg·(g·min)−1 were achieved with 100 mg DILs for 5 mL of 5 mg/L Cd2+ and Pb2+ solutions. Besides, the extraction efficiencies of Cd2+ and Pb2+ were respectively up to 95.48% and 98.46%, when the volumes of the simulated wastewater were expanded by a factor of 20 at a constant extraction phase ratio (1000 mg DILs for 50 mL of 5 mg/L Cd2+ or Pb2+ solutions). The reusability of the novel DILs was successfully proved by the back-extraction experiments with 0.5 M HNO 3. Finally, taking Cd2+ extraction as an example, the extraction mechanism based on FTIR analysis and quantum chemical calculations showed that both S and O atoms in the anions of DILs had physical and quasi-chemical interactions with Cd2+, which were stronger than the electrostatic attraction. [Display omitted] • Two types of non-liquid hydrophobic novel dicationic ionic liquids for heavy metal extraction were synthesized. • As-Prepared ionic liquids demonstrated high efficiency on extracting Cd2+ and Pb2+ from the simulated wastewater. • [HxPrHx][BTB] 2 possessed extraction rates up to 0.45 and 0.53 mg/g·min−1 for Cd2+ and Pb2+ in the studied condition. • [HxPrHx][BTB] 2 exhibited excellent reversibility after five cycles of Cd2+ extraction/back-extraction process. [ABSTRACT FROM AUTHOR]

In this study, the behavior of heavy metal transformation during the co-thermal treatment of hazardous waste incineration fly ash (HWIFA) and Fe-containing hazardous waste (including hazardous waste incineration bottom slag (HWIBS) and electroplating sludge (ES)) was investigated. The findings demonstrated that such a treatment effectively reduced the static leaching toxicity of Cr and Pb. Moreover, when the treatment temperature exceeded 1000 °C, the co-thermal treated sample exhibited low concentrations of dynamically leached Cr, Pb, and Zn, indicating that these heavy metals were successful detoxified. Thermodynamic analyses and phase transformation results suggested that the formation of spinel and the gradual disappearance of chromium dioxide in the presence of Fe-containing hazardous wastes contributed to the solidification of chromium. Additionally, the efficient detoxification of Pb and Zn was attributed to their volatilization and entry into the liquid phase during the co-thermal treatment process. Therefore, this study sets an excellent example of the co-thermal treatment of hazardous wastes and the control of heavy metal pollution during the treatment process. • Hazardous waste co-thermal treatment achieves efficient detoxification. • Fe-containing hazardous waste promotes the detoxification of Cr, Pb and Zn. • Spinel forming is the reason for the solidification of Cr by co-thermal treatment. [ABSTRACT FROM AUTHOR]

Pyrolysis is a promising method to treat antibiotic fermentation residue (AFR), a hazardous waste in China, with the benefits of detoxification and resource recycling. However, the application of the AFR-derived biochar has been limited yet, restricting the use of pyrolysis to treat AFR. Herein, for the first time, we reported the use of magnetic biochars derived from vancomycin fermentation residue to rapidly and efficiently co-adsorb multiple heavy metals from diverse types of water with complex matrices. The biochar prepared at 700 °C (labeled as VBC700) exhibited high affinity and selectivity for multiple heavy metals, especially for Ag(I), Hg(II), Pb(II), and Cu(II). The kinetics for Ag(I), Hg(II), and Pb(II) were ultrafast with an equilibrium time of only 5 min, while those for Cu(II) were relatively slower. The maximum adsorption capacity calculated from the Langmuir model for Ag(I), Hg(II), Pb(II), and Cu(II) reached 177.4, 105.9, 387.1, 124.5 mg/g, respectively, which were superior to much previously reported adsorbents. Impressively, Na(I), K(I), Ca(II), Mg(II), and salinity did not affect the capture of these heavy metals, and thus >99% of Ag(I), Pb(II), and Cu(II) were concurrently removed from complex water matrices including seawater, which has rarely been reported before. Furthermore, VBC700 remained high adsorption performance at pH ≥ 3. The adsorption mechanisms included ion exchange, precipitation, and inner-sphere complexation. Overall, the results demonstrate that VBC700 would be an excellent adsorbent to co-capture multiple heavy metals from diverse types of water, highlighting the feasibility of using pyrolysis to achieve a win-win goal for AFR management and heavy metal pollution control. [Display omitted] • Magnetic biochars were prepared from vancomycin fermentation residue. • Rapid kinetics and high capacities for Ag+, Hg2+, Cu2+, and Pb2+ were observed. • More than 99% of Ag+, Hg2+, and Pb2+ were simultaneously removed from seawater. • Sorption was achieved by precipitation/ion exchange and inner-sphere complexation. [ABSTRACT FROM AUTHOR]