1. Multi-imaging platform for rhizosphere studies: Phosphorus and oxygen fluxes.
- Author
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Li, Xi-Yuan, Li, Su-Qing, Jiang, Yi-Fan, Yang, Qiong, Zhang, Jian-Chao, Kuzyakov, Yakov, Teng, H. Henry, and Guan, Dong-Xing
- Subjects
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ACID phosphatase , *ROOT development , *RHIZOSPHERE , *PHOSPHORUS , *OXYGEN , *GEOLOGIC hot spots , *EXTRACELLULAR enzymes - Abstract
Rhizosphere is a soil volume of high spatio-temporal heterogeneity and intensive plant-soil-microbial interactions, for which visualization and process quantification is of highest scientific and applied relevance, but still very challenging. A novel methodology for quick assessment of two-dimensional distribution of available phosphorus (P) in rhizosphere was suggested, tested, and development up to the application platform. Available P was firstly trapped by an in-situ diffusive gradients in thin-films (DGT) sampler with precipitated zirconia as the binding gel, and subsequently, the loaded gel was analyzed with an optimized colorimetric imaging densitometry (CID). The imaging platform was established linking: i) DGT, ii) planar optode, and iii) soil zymography techniques to simultaneously determine available P, oxygen, and acid phosphatase in rhizosphere at sub-millimeter spatial scales. The DGT identified available P level in rice rhizosphere were spatially overlapping to the localized redox hotspots and phosphatase activity. The spatial relationship between available P and acid phosphatase activity was dependent on root development. The root radial oxygen loss (ROL) remained active during the experimental observations (2–3 days), while a flux of available P of 10 pg cm−2 s−1 was visualized within 2–3 mm of roots, confirming the correlative response of rice roots to oxygen secretion and P uptake. Summarizing, the established imaging platform is suitable to capture spatial heterogeneity and temporal dynamics of root activities, nutrient bioavailability, ROL and enzyme activities in rhizosphere. [Display omitted] • Quick establishment of the two-dimensional distribution of available P was realized. • A versatile platform was established for imaging multiple analytes in rhizosphere. • Rhizosphere P dynamics were linked to localized redox conditions and root activity. • Relationship between P and acid phosphatase activities varied as root developed. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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