1. Comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with Enterococcus faecalis: an in vitro study.
- Author
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de Almeida AP, Souza MA, Miyagaki DC, Dal Bello Y, Cecchin D, and Farina AP
- Subjects
- Animals, Bacterial Load drug effects, Bacteriological Techniques, Cattle, Root Canal Preparation methods, Anti-Bacterial Agents pharmacology, Calcium Compounds pharmacology, Dental Pulp Cavity microbiology, Enterococcus faecalis drug effects, Root Canal Irrigants pharmacology, Sodium Hypochlorite pharmacology, Therapeutic Irrigation methods, Ultrasonic Therapy methods
- Abstract
Introduction: The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl]2) and sodium hypochlorite (NaOCl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis., Methods: The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis, and incubated for 30 days. The samples were divided into 6 groups (n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl)2; G5: 2.5% NaOCl with ultrasonic activation; and G6: 2.5% Ca(OCl)2 with ultrasonic activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05)., Results: Groups 1 and 2 showed the highest mean contamination (3.26 log10 CFU/mL and 2.69 log10 CFU/mL, respectively), which was statistically different from all other groups (P < .05). Group 6 (Ca[OCl]2 + US) showed the lowest mean contamination (1.00 log10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl]2), and 5 (NaOCl + US) (P < .05)., Conclusions: Ca(OCl)2 as well as passive ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment., (Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
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