1. Myo-inositol alters 13C-labeled fatty acid metabolism in human placental explants.
- Author
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Watkins, Oliver C., Islam, Mohammed Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K., Karnani, Neerja, Godfrey, Keith M., Lewis, Rohan M., Wenk, Markus R., and Shiao-Yng Chan
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FATTY acids ,PALMITIC acid ,METABOLISM ,GESTATIONAL diabetes ,PATHOLOGICAL physiology - Abstract
We postulate that myo-inositol, a proposed intervention for gestational diabetes, affects transplacental lipid supply to the fetus. We investigate d the effect of myoinositol on fatty acid processing in human placental explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic acid,
13 C-oleic acid and13 C-docosahexaenoic acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled fatty acids into in dividual lipids was quantified by liquid chromatography mass spectrometry. At 24 h, myo-inositol increased the amount of13 C-palmitic acid and13 C-oleic-acid labeled lipids (median fold change relative to control = 1). Significant effects were seen with 30 µM myo-inositol (physiological) for13 C-palmitic acid-lysophosphatidylcholines (1.26) and13 C-palmitic acidphosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased13 C-oleic-acidlipids but decreased13 C-palmitic acid and13 C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for13 C-oleic-acid-phosphatidylcholines (1.25),13 C-oleic-acid-phosphatidylethanolamines (1.37) and13 C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for13 C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same13 C-fatty acid showed similar responses when tested at the same time point, suggesting myo-inositol alters upstream processes such as fatty acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences. [ABSTRACT FROM AUTHOR]- Published
- 2019
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