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Viral hepatitis is a major public health problem worldwide and in Burkina Faso. Traditional healers are widely involved in its therapeutic management. The aim of our study was to investigate the knowledge attitudes and practices of traditional healers on viral hepatitis. This is a descriptive cross-acted study of February the 1st to April the 30th, 2015 with 100 traditional practitioners affiliated to the two associations of traditional practitioners in Bobo Dioulasso. The knowledge on viral hepatitis appeared generally unsufficient in 95%. We recorded a good level of knowledge on the definition in 57% of viral etiology in 8.8%, on the kinds of viral hepatitis in 3.5%. The main routes of contamination pathways namely sexual and blood were known to 44.4% and 11.1% respectively. The mother-child transmission was known only 5.5%. Regarding the clinical, jaundice and fever were known only to 14,03% and 3.5% respectively. Vaccination was only known to only 26.4%. Literacy has been associated with a good overall knowledge of viral hepatitis (p=0.01) as opposed to ancienty in practice (0.16). The plants are central to the therapeutic arsenal for 91.5% of TP. Most cited were Chrysantheliumamericana, Combretummicranthum, Cochlespermumtinctorium, Anogeissusleiocarpus, Terminaliamacropter. The leaves were the most used (62.5%). The form of the most common presentation was decoction (82.6%). The route of administration the most recommended was oral (91.3). Medical Analyses were the main reason for reference to modern medicine (62.5%). This study demonstrated a knowledge of TP deficiency on viral hepatitis. The knowledge was associated with literacy. The plants dominate in the armamentarium of the TP, some found in our study should be the subject of pharmacological studies.