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Start Over Author "Eun J" Journal journal of dairy science
17 results on '"Eun J"'

4. Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine

Author

Amaro, F.X., primary, Kim, D., additional, Restelatto, R., additional, Carvalho, P., additional, Arriola, K., additional, Duvalsaint, E.J.C., additional, Cervantes, A.P., additional, Jiang, Y., additional, Agarussi, M.C.N., additional, Silva, V.P., additional, Adesogan, A.T., additional, Ferraretto, L.F., additional, Staples, C.R., additional, Eun, J.-S., additional, Moon, J.O., additional, and Vyas, D., additional

Published
2022
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7. Increased Levels of LPS-Binding Protein in Bovine Blood and Milk Following Bacterial Lipopolysaccharide Challenge

Author

William R. Hare, M.J. Paape, Eun J Sohn, and Douglas D. Bannerman

Subjects
Lipopolysaccharides, medicine.medical_specialty, Gram-negative bacteria, Lipopolysaccharide, Neutrophils, CD14, Lipopolysaccharide Receptors, Microbiology, Leukocyte Count, chemistry.chemical_compound, Mammary Glands, Animal, Internal medicine, Lactation, Escherichia coli, Genetics, medicine, Animals, Mastitis, Bovine, Toll-like receptor, Membrane Glycoproteins, biology, Interleukin-8, Acute-phase protein, biology.organism_classification, Kinetics, Milk, medicine.anatomical_structure, Endocrinology, chemistry, biology.protein, Cattle, Female, lipids (amino acids, peptides, and proteins), Animal Science and Zoology, Carrier Proteins, Bacterial outer membrane, Lipopolysaccharide binding protein, Acute-Phase Proteins, Food Science
Abstract

Several species of gram-negative bacteria, including Escherichia coli, Klebsiella pneumoniae, and various species of Enterobacter, are common mastitis pathogens. All of these bacteria are characterized by the presence of endotoxin or lipopolysaccharide (LPS) in their outer membrane. The bovine mammary gland is highly sensitive to LPS, and LPS has been implicated, in part, in the pathogenesis of gram-negative mastitis. Recognition of LPS is a key event in the innate immune response to gram-negative infection and is mediated by the accessory molecules CD14 and LPS-binding protein (LBP). The objective of the current study was to determine whether LBP levels increased in the blood and mammary gland following LPS challenge. The left and right quarters of five midlactating Holstein cows were challenged with either saline or LPS (100 microg), respectively, and milk and blood samples collected. Basal levels of plasma and milk LBP were 38 and 6 microg/ml, respectively. Plasma LBP levels increased as early as 8 h post-LPS challenge and reached maximal levels of 138 microg/ ml by 24 h. Analysis of whey samples derived from LPS-treated quarters revealed an increase in milk LBP by 12 h. Similar to plasma, maximal levels of milk LBP (34 microg/ml) were detected 24 h following the initial LPS challenge. Increments in milk LBP levels paralleled a rise in soluble CD14 (sCD14) levels and initial rises in the levels of these proteins were temporally coincident with maximal neutrophil recruitment to the inflamed gland. Because LBP and sCD14 are known to enhance LPS-induced host cell activation and to facilitate detoxification of LPS, these data are consistent with a role for these molecules in mediating mammary gland responses to LPS.

Published
2003

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