1. Low mannose-binding lectin (MBL) is associated with paediatric inflammatory bowel diseases and ileal involvement in patients with Crohn disease
- Author
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Peter L. Lakatos, Eniko Solyom, Dolóresz Szabó, Kriszta Molnár, Agnes Horvath, Marianne Polgár, Márta Kovács, Katalin Eszter Müller, Gábor Veres, Antal Dezsofi, Éva Nemes, Piroska Bodi, Silvia Jacobsen, Áron Cseh, Mária Papp, and András Arató
- Subjects
Adult ,Male ,Adolescent ,Nod2 Signaling Adaptor Protein ,Mannose-Binding Lectin ,Severity of Illness Index ,Inflammatory bowel disease ,Statistics, Nonparametric ,Antibodies, Antineutrophil Cytoplasmic ,Young Adult ,Sex Factors ,Crohn Disease ,Ileum ,NOD2 ,Confidence Intervals ,Odds Ratio ,medicine ,Humans ,Prospective Studies ,Child ,Mannan-binding lectin ,Crohn's disease ,P-ANCA ,business.industry ,Gastroenterology ,Autoantibody ,General Medicine ,medicine.disease ,MBL deficiency ,Ulcerative colitis ,digestive system diseases ,C-Reactive Protein ,Case-Control Studies ,Child, Preschool ,Immunology ,Colitis, Ulcerative ,Female ,business ,Biomarkers - Abstract
Mannose-binding lectin (MBL) is a pattern-recognition molecule of the innate immune system and may be involved in the pathogenesis of inflammatory bowel disease (IBD). Our aim was to assess the prevalence of MBL deficiency in a cohort of patients with paediatric-onset IBD and study whether it is associated with the clinical manifestations, serum antibody formation, or genetic factors.This prospective study included 159 paediatric patients (mean age: 14.0 years) with IBD [107 patients with Crohn disease (CD) and 52 patients with ulcerative colitis (UC)]. Furthermore, 95 controls were investigated. Serum samples were determined for MBL by enzyme-linked immunosorbent assay (ELISA) and for serologic markers [autoantibodies against Saccharomyces cerevisiae (ASCA) and perinuclear components of neutrophils (pANCA)] by indirect immunofluorescent assay. NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism.The MBL serum concentration was significantly lower in IBD patients(both with CD and UC) compared to controls (IBD, p=0.007, CD, p=0.04, UC p=0.004). Prevalence of low MBL level (500 ng/mL) was significantly higher in both CD and UC groups compared to controls (p=0.002 and p=0.006). Furthermore, low MBL level was associated with isolated ileal involvement (p=0.01) and MBL deficiency (100 ng/mL) with male gender (p=0.004) in patients with CD. We failed to confirm any correlation between MBL deficiency and serum autoantibodies or NOD2/CARD15 variants.Our results suggest that low MBL associated with paediatric-onset IBD and ileal CD may be considered an additional marker of the IBD pathogenesis.
- Published
- 2013