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Background: Reverse the fat accumulation and to improve body contouring with more safely than invasive procedures has become a necessary therapeutic approach, but little detailed when using LED photobiomodulation., Aims: To evaluate an application protocol with two different wavelengths (red and infrared LED) consecutively in the abdomen region alone or associated with the dermocosmetics application with lipolytic properties., Methods: Ninety patients with a significant amount of adipose tissue in the abdomen region were selected and randomized into three groups: Sham (SG), in which patients received a simulation of the treatment; LED (LG), in which participants received the application with LED; LED with dermocosmetic (LDG), in which the participants underwent a combination of LED and dermocosmetic treatment. The following assessments were carried out: anthropometric data (weight, height, perimetry, and adipometry); ultrasound examination to adipose layer; patient satisfaction questionnaire; histology of a donated a tissue sample., Results: The main findings were a decrease in umbilical perimetry, a significant decrease in the body fat layer determined by ultrasound and significant histological changes that indicated an improvement in the appearance of the skin and an increase in the amount of macrophages in the subcutaneous layer. Histological data also showed an improvement in the appearance of the skin with an increase in collagen deposition and an increase in macrophages in the subcutaneous layer., Conclusions: The LED phototherapy application protocol, both alone and in association with the dermocosmetic, with the emission of two consecutive wavelengths, was effective in reducing the fat tissue in the abdomen region., (© 2024 The Author(s). Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.)