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The COVID-19 disease is presently a matter of global public health concern as it could be potentially fatal. Health workers have at the moment, inadequate knowledge of prevention measures, and their erroneous practices may directly increase the risk of spread. As a result, matters are complicated further as far as the chain of infection is concerned. The present cross-sectional study was conducted with 494 Health Worker participants in Cyprus between the 1st and 20th of May and the data were collected via an online questionnaire. The survey was created using information from a previous study as well as current directives published on the WHO website. The statistical software SPSS 22.0 was used for data analysis. The ANOVA test was used for the comparison of quantitative variables, while the Kruskal–Wallis test was used in cases of non-parametric distribution. The Pearson or Spearman coefficients were used to test correlation and the levels of materiality were set to 0,05. Women constituted 66,7% of the participants and 44,8% of all participants were between the age of 30–39 years old. The majority (75,4%) of the participants were Nursing Officers and 44,5% held postgraduate qualifications. HWs appeared to have a positive perception (x̅ = 3.58, SD = 0.53, MR = 1–5) regarding the course of the pandemic and their knowledge of the virus was found to be generally satisfactory. The findings of the current study indicate that in case of a similar global public health crisis, certain educational interventions should be implemented. This could be achieved with the use of an appropriate strategy. As a result, all demographic-working strata of health professionals would be well-educated and informed. [ABSTRACT FROM AUTHOR]