1. Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces
- Author
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Hemanth Kumar Naguvanahalli Krishnappa, Ashwini Bagepalli Shivaram, Satish Anandan, Murali Mahadevamurthy, Udayashankar Arakere Chunchegowda, Eswaraiah Ganadhal Chanappa, Lakshmeesha Thimappa Ramachndrappa, Sreelatha Gopalakrishna Lalitha, Brijesh Singh Sudarshana, and Niranjana Siddapura Ramachandrappa
- Subjects
biology ,Scanning electron microscope ,Energy-dispersive X-ray spectroscopy ,chemistry.chemical_element ,Nanoparticle ,02 engineering and technology ,General Chemistry ,Zinc ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,biology.organism_classification ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Passiflora subpeltata ,chemistry ,medicine ,General Materials Science ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Antibacterial activity ,Escherichia coli ,Nuclear chemistry - Abstract
The current study was undertaken to investigate the antibacterial (against molecular characterized E. coli isolated from poultry faeces) potential of biosynthesized zinc oxide nanoparticles (ZnO-NPs) from Passiflora subpeltata Ortega aqueous leaf extract. The biosynthesized nanoparticles were subjected to physico-chemical characterization to study shape, size and purity by UV–Vis spectroscopy, X-Ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The molecular identification of isolated E. coli from faeces samples was carried out by using 16–23s rRNA primers. The results of the physico-chemical characterization revealed that the biosynthesized nanoparticles were of 93.7% purity with an average size between 45 and 50 nm. The ZnO-NPs offered significant inhibition against the isolated Gram-negative E. coli with MIC at 62.5 µg mL−1 concentration. The antibacterial potential of ZnO NPs against E. coli has also been investigated by the cell viability test, and further the effects of ZnO NPs on bacterial morphological structures was analysed by SEM and TEM.
- Published
- 2020