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Start Over Author "Drosten, Christian" Journal journal of clinical virology
15 results on '"Drosten, Christian"'

11. Identification of essential outstanding questions for an adequate European laboratory response to Ebolavirus Zaire West Africa 2014

Author

Reusken, Chantal, primary, Niedrig, Matthias, additional, Pas, Suzan, additional, Anda, Pedro, additional, Baize, Sylvain, additional, Charrel, Remi, additional, Di Caro, Antonino, additional, Drosten, Christian, additional, Fernandez-Garcia, Maria Dolores, additional, Franco, Leticia, additional, Gunther, Stephan, additional, Leparc-Goffart, Isabelle, additional, Martina, Byron, additional, Pannetier, Delphine, additional, Papa, Anna, additional, Sanchez-Seco, Maria Paz, additional, Vapalahti, Olli, additional, and Koopmans, Marion, additional

Published
2015
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13. Hepatitis E virus infections in HIV-infected patients in Ghana and Cameroon

Author

Feldt, Torsten, primary, Sarfo, Fred Stephen, additional, Zoufaly, Alexander, additional, Phillips, Richard Odame, additional, Burchard, Gerd, additional, van Lunzen, Jan, additional, Jochum, Johannes, additional, Chadwick, David, additional, Awasom, Charles, additional, Claussen, Lisa, additional, Drosten, Christian, additional, Drexler, Jan Felix, additional, and Eis-Hübinger, Anna Maria, additional

Published
2013
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14. Singleplex real-time RT-PCR for detection of influenza A virus and simultaneous differentiation of A/H1N1v and evaluation of the RealStar influenza kit

Author

Panning, Marcus, primary, Baumgarte, Sigrid, additional, Laue, Thomas, additional, Bierbaum, Sibylle, additional, Raith, Sabine, additional, Drexler, Jan Felix, additional, Helmer, Angelika, additional, Falcone-Kapper, Valeria, additional, Kochs, Georg, additional, Campe, Hartmut, additional, Huzly, Daniela, additional, Eis-Hübinger, Anna Maria, additional, and Drosten, Christian, additional

Published
2011
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15. Performance and clinical validation of the RealStar® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.

Author

Corman, Victor Max, Ölschläger, Stephan, Wendtner, Clemens-Martin, Drexler, Jan Felix, Hess, Markus, and Drosten, Christian

Subjects
*MIDDLE East respiratory syndrome, *RESPIRATORY infections, *CORONAVIRUSES, *RNA viruses, *RNA analysis, *DIAGNOSTIC reagents & test kits, *GENE amplification, *DIAGNOSIS
Abstract

Abstract: Background: A highly pathogenic human coronavirus causing respiratory disease emerged in the Middle East region in 2012. In-house molecular diagnostic methods for this virus termed Middle East respiratory syndrome coronavirus (MERS-CoV) allowed sensitive MERS-CoV RNA detection in patient samples. Fast diagnosis is important to manage human cases and trace possible contacts. Objectives: The aim of this study was to improve the availability of existing nucleic acid amplification-based diagnostic methods for MERS-CoV infections by providing a real-time RT-PCR kit, including an internal control and two target regions recommended by the World Health Organization (WHO). And to validate this kit (RealStar® MERS-CoV RT-PCR kit 1.0, Altona Diagnostics GmbH, Hamburg, Germany) using clinical samples of one MERS-CoV case from Munich and respiratory samples of patients with other respiratory diseases. Study design: An internal amplification control was included into the RT-PCR assays targeting the genomic region upstream of the Envelope gene (upE) and within open reading frame (ORF) 1A. Based on these assays, a ready-to-use real-time RT-PCR kit featuring both the upE and ORF1A assays was developed, validated and compared to the established in-house versions. Results: The performance of both RT-PCR assays included in the kit is comparable to the in-house assays. They show high analytical sensitivity (upE: 5.3 copies/reaction; ORF1A: 9.3 copies/reaction), no cross-reactivity with other respiratory pathogens and detected MERS-CoV RNA in patient samples in almost the same manner as the in-house versions. Conclusion: The kit is a valuable tool for assisting in the rapid diagnosis, patient management and epidemiology of suspected MERS-CoV cases. [Copyright &y& Elsevier]

Published
2014
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