1. KRAS mutational status of endoscopic biopsies matches resection specimens
- Author
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Qinghua Yang, Jason Schmidt, Liza Dejesa-Jamanila, Richard H. Lash, Genvieve Soucy, Robert D. Odze, Keely Arnold, and Christine Kuslich
- Subjects
medicine.medical_specialty ,Pathology ,Genotype ,Colon ,Colorectal cancer ,Biopsy ,Adenocarcinoma ,medicine.disease_cause ,Pathology and Forensic Medicine ,Resection ,Proto-Oncogene Proteins p21(ras) ,symbols.namesake ,Proto-Oncogene Proteins ,Humans ,Medicine ,Mutational status ,Sanger sequencing ,medicine.diagnostic_test ,business.industry ,Rectum ,Sequence Analysis, DNA ,General Medicine ,medicine.disease ,digestive system diseases ,Mutational analysis ,Mutation ,ras Proteins ,symbols ,Female ,Radiology ,KRAS ,Colorectal Neoplasms ,business ,Kras mutation - Abstract
AimsThis study was performed to determine systematically whether KRAS mutational analysis in biopsy tissue is a reliable indicator of KRAS status in subsequent corresponding resection specimens.Methods30 colorectal cancer (CRC) patients with biopsy and corresponding subsequent surgical resection specimens were studied. KRAS mutational analysis was performed on each biopsy sample as well as two separate samples from each resection specimen by PCR and Sanger sequencing.ResultsOverall, KRAS mutations were identified in 12/30 (40%) of the tumours. There was 100% correlation between biopsy and resection specimens regarding the presence or absence of KRAS mutations. In fact, the same point mutation was identified in both biopsy and corresponding resection specimens in 12/12 (100%) cases. In addition, in two cases, there were two different point mutations detected within the same biopsy specimen.ConclusionThis study shows perfect correlation between KRAS mutation status in biopsy and resection specimens from an individual patient, and suggests that biopsy material is adequate for KRAS mutational analysis in CRC patients.
- Published
- 2012
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