Search

Your search keyword '"PERSING, DAVID H."' showing total 40 results
Start Over Author "PERSING, DAVID H." Journal journal of clinical microbiology
40 results on '"PERSING, DAVID H."'

2. Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV

Author

Södersten, Erik, primary, Ongarello, Stefano, additional, Mantsoki, Anna, additional, Wyss, Romain, additional, Persing, David H., additional, Banderby, Sara, additional, Strömqvist Meuzelaar, Linda, additional, Prieto, Jacqueline, additional, Gnanashanmugam, Devasena, additional, Khatri, Purvesh, additional, Schumacher, Samuel G., additional, and Denkinger, Claudia M., additional

Published
2021
Full Text
View/download PDF

3. Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2/Flu/RSV Test

Author

Mostafa, Heba H., primary, Carroll, Karen C., additional, Hicken, Rachel, additional, Berry, Gregory J., additional, Manji, Ryhana, additional, Smith, Elizabeth, additional, Rakeman, Jennifer L., additional, Fowler, Randal C., additional, Leelawong, Mindy, additional, Butler-Wu, Susan M., additional, Quintero, David, additional, Umali-Wilcox, Minette, additional, Kwiatkowski, Robert W., additional, Persing, David H., additional, Weir, Fred, additional, and Loeffelholz, Michael J., additional

Published
2021
Full Text
View/download PDF

4. Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test

Author

Loeffelholz, Michael J., primary, Alland, David, additional, Butler-Wu, Susan M., additional, Pandey, Utsav, additional, Perno, Carlo Frederico, additional, Nava, Alice, additional, Carroll, Karen C., additional, Mostafa, Heba, additional, Davies, Emma, additional, McEwan, Ashley, additional, Rakeman, Jennifer L., additional, Fowler, Randal C., additional, Pawlotsky, Jean-Michel, additional, Fourati, Slim, additional, Banik, Sukalyani, additional, Banada, Padmapriya P., additional, Swaminathan, Shobha, additional, Chakravorty, Soumitesh, additional, Kwiatkowski, Robert W., additional, Chu, Victor C., additional, Kop, JoAnn, additional, Gaur, Rajiv, additional, Sin, Mandy L. Y., additional, Nguyen, Duy, additional, Singh, Simranjit, additional, Zhang, Na, additional, and Persing, David H., additional

Published
2020
Full Text
View/download PDF

9. Evaluation of a Rapid and Completely Automated Real-Time Reverse Transcriptase PCR Assay for Diagnosis of Enteroviral Meningitis

Author

Nolte, Frederick S., primary, Rogers, Beverly B., additional, Tang, Yi-Wei, additional, Oberste, M. Steven, additional, Robinson, Christine C., additional, Kehl, K. Sue, additional, Rand, Kenneth A., additional, Rotbart, Harley A., additional, Romero, Jose R., additional, Nyquist, Ann-Christine, additional, and Persing, David H., additional

Published
2011
Full Text
View/download PDF

10. Impact of Strain Type on Detection of Toxigenic Clostridium difficile: Comparison of Molecular Diagnostic and Enzyme Immunoassay Approaches

Author

Tenover, Fred C., primary, Novak-Weekley, Susan, additional, Woods, Christopher W., additional, Peterson, Lance R., additional, Davis, Thomas, additional, Schreckenberger, Paul, additional, Fang, Ferric C., additional, Dascal, Andre, additional, Gerding, Dale N., additional, Nomura, Jim H., additional, Goering, Richard V., additional, Akerlund, Thomas, additional, Weissfeld, Alice S., additional, Baron, Ellen Jo, additional, Wong, Edith, additional, Marlowe, Elizabeth M., additional, Whitmore, Joseph, additional, and Persing, David H., additional

Published
2010
Full Text
View/download PDF

12. Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology

Author

Helb, Danica, primary, Jones, Martin, additional, Story, Elizabeth, additional, Boehme, Catharina, additional, Wallace, Ellen, additional, Ho, Ken, additional, Kop, JoAnn, additional, Owens, Michelle R., additional, Rodgers, Richard, additional, Banada, Padmapriya, additional, Safi, Hassan, additional, Blakemore, Robert, additional, Lan, N. T. Ngoc, additional, Jones-López, Edward C., additional, Levi, Michael, additional, Burday, Michele, additional, Ayakaka, Irene, additional, Mugerwa, Roy D., additional, McMillan, Bill, additional, Winn-Deen, Emily, additional, Christel, Lee, additional, Dailey, Peter, additional, Perkins, Mark D., additional, Persing, David H., additional, and Alland, David, additional

Published
2010
Full Text
View/download PDF

17. Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Author

Qian, Qinfang, primary, Tang, Yi-Wei, additional, Kolbert, Christopher P., additional, Torgerson, Catherine A., additional, Hughes, John G., additional, Vetter, Emily A., additional, Harmsen, W. Scott, additional, Montgomery, Stacy O., additional, Cockerill, Franklin R., additional, and Persing, David H., additional

Published
2001
Full Text
View/download PDF

18. Serological Expression Cloning and Immunological Evaluation of MTB48, a Novel Mycobacterium tuberculosis Antigen

Author

Lodes, Michael J., primary, Dillon, Davin C., additional, Mohamath, Raodoh, additional, Day, Craig H., additional, Benson, Darin R., additional, Reynolds, Lisa D., additional, McNeill, Patricia, additional, Sampaio, Diana Pedral, additional, Skeiky, Yasir A. W., additional, Badaro, Roberto, additional, Persing, David H., additional, Reed, Steven G., additional, and Houghton, Raymond L., additional

Published
2001
Full Text
View/download PDF

19. Serodiagnosis of Human Granulocytic Ehrlichiosis by Using Novel Combinations of Immunoreactive Recombinant Proteins

Author

Lodes, Michael J., primary, Mohamath, Raodoh, additional, Reynolds, Lisa D., additional, McNeill, Patricia, additional, Kolbert, Christopher P., additional, Bruinsma, Elizabeth S., additional, Benson, Darin R., additional, Hofmeister, Eric, additional, Reed, Steven G., additional, Houghton, Raymond L., additional, and Persing, David H., additional

Published
2001
Full Text
View/download PDF

21. Comparison of Protein A Gene Sequencing with Pulsed-Field Gel Electrophoresis and Epidemiologic Data for Molecular Typing of Methicillin-Resistant Staphylococcus aureus

Author

Tang, Yi-Wei, primary, Waddington, Michael G., additional, Smith, Douglas H., additional, Manahan, Janice M., additional, Kohner, Peggy C., additional, Highsmith, Leanne M., additional, Li, Haijing, additional, Cockerill, Franklin R., additional, Thompson, Rodney L., additional, Montgomery, Stacy O., additional, and Persing, David H., additional

Published
2000
Full Text
View/download PDF

29. Clinical Evaluation of the Gen-Probe Amplified Direct Test for Detection of Mycobacterium tuberculosis Complex Organisms in Cerebrospinal Fluid

Author

Lang, Anne M., primary, Feris-Iglesias, Jesus, additional, Pena, Chabela, additional, Sanchez, Jacqueline F., additional, Stockman, Leslie, additional, Rys, Paul, additional, Roberts, Glenn D., additional, Henry, Nancy K., additional, Persing, David H., additional, and Cockerill, Franklin R., additional

Published
1998
Full Text
View/download PDF

30. Comparison of Strain Typing Results for Clostridium difficileIsolates from North America

Author

Tenover, Fred C., Åkerlund, Thomas, Gerding, Dale N., Goering, Richard V., Boström, Therése, Jonsson, Anna-Maria, Wong, Edith, Wortman, Alan T., and Persing, David H.

Abstract

ABSTRACTAccurate strain typing is critical for understanding the changing epidemiology of Clostridium difficileinfections. We typed 350 isolates of toxigenic C. difficilefrom 2008 to 2009 from seven laboratories in the United States and Canada. Typing was performed by PCR-ribotyping, pulsed-field gel electrophoresis (PFGE), and restriction endonuclease analysis (REA) of whole-cell DNA. The Cepheid Xpert C. difficiletest for presumptive identification of 027/NAP1/BI isolates was also tested directly on original stool samples. Of 350 isolates, 244 (70%) were known PCR ribotypes, 224 (68%) were 1 of 8 common REA groups, and 187 (54%) were known PFGE types. Eighty-four isolates typed as 027, NAP1, and BI, and 83 of these were identified as presumptive 027/NAP1/BI by Xpert C. difficile. Eight additional isolates were called presumptive 027/NAP1/BI by Xpert C. difficile, of which three were ribotype 027. Five PCR ribotypes contained multiple REA groups, and three North American pulsed-field (NAP) profiles contained both multiple REA groups and PCR ribotypes. There was modest concordance of results among the three methods for C. difficilestrains, including the J strain (ribotype 001 and PFGE NAP2), the toxin A-negative 017 strain (PFGE NAP9 and REA type CF), the 078 animal strain (PFGE NAP7 and REA type BK), and type 106 (PFGE NAP11 and REA type DH). PCR-ribotyping, REA, and PFGE provide different but overlapping patterns of strain clustering. Unlike the other methods, the Xpert C. difficile027/NAP1/BI assay gave results directly from stool specimens, required only 45 min to complete, but was limited to detection of a single strain type.

Published
2011
Full Text
View/download PDF

31. Impact of Strain Type on Detection of Toxigenic Clostridium difficile:Comparison of Molecular Diagnostic and Enzyme Immunoassay Approaches

Author

Tenover, Fred C., Novak-Weekley, Susan, Woods, Christopher W., Peterson, Lance R., Davis, Thomas, Schreckenberger, Paul, Fang, Ferric C., Dascal, Andre, Gerding, Dale N., Nomura, Jim H., Goering, Richard V., Akerlund, Thomas, Weissfeld, Alice S., Baron, Ellen Jo, Wong, Edith, Marlowe, Elizabeth M., Whitmore, Joseph, and Persing, David H.

Abstract

ABSTRACTA multicenter clinical trial assessed the performance of the Cepheid Xpert C. difficileassay on stool specimens collected from patients suspected of having Clostridium difficileinfection (CDI). A total of 2,296 unformed stool specimens, collected from seven study sites, were tested by Xpert C. difficileenrichment culture followed by cell culture cytotoxicity testing of the isolates (i.e., toxigenic culture with enrichment) and the study sites' standard C. difficiletest methods. The methods included enzyme immunoassay (EIA), direct cytotoxin testing, and two- and three-step algorithms using glutamate dehydrogenase (GDH) screening followed by either EIA or EIA and an in-house PCR assay. All C. difficilestrains were typed by PCR-ribotyping. Compared to results for toxigenic culture with enrichment, the sensitivity, specificity, and positive and negative predictive values of the Xpert assay were 93.5, 94.0, 73.0, and 98.8%, respectively. The overall sensitivity of the EIAs compared to that of enrichment culture was 60.0%, and the sensitivity of combined GDH algorithms was 72.9%; both were significantly lower than that of Xpert C. difficile(P< 0.001 and P= 0.03, respectively). The sensitivity of the EIA was significantly lower than that of the Xpert C. difficileassay for detection of ribotypes 002, 027, and 106 (P< 0.0001, P< 0.0001, and P= 0.004, respectively, Fisher's exact test), and the sensitivity of GDH algorithms for ribotypes other than 027 was lower than that for Xpert C. difficile(P< 0.001). The Xpert C. difficileassay is a simple, rapid, and accurate method for detection of toxigenic C. difficilein unformed stool specimens and is minimally affected by strain type compared to EIA and GDH-based methods.

Published
2010
Full Text
View/download PDF

32. Rapid Detection of Mycobacterium tuberculosisand Rifampin Resistance by Use of On-Demand, Near-Patient Technology

Author

Helb, Danica, Jones, Martin, Story, Elizabeth, Boehme, Catharina, Wallace, Ellen, Ho, Ken, Kop, JoAnn, Owens, Michelle R., Rodgers, Richard, Banada, Padmapriya, Safi, Hassan, Blakemore, Robert, Lan, N. T. Ngoc, Jones-Lo´pez, Edward C., Levi, Michael, Burday, Michele, Ayakaka, Irene, Mugerwa, Roy D., McMillan, Bill, Winn-Deen, Emily, Christel, Lee, Dailey, Peter, Perkins, Mark D., Persing, David H., and Alland, David

Abstract

ABSTRACTCurrent nucleic acid amplification methods to detect Mycobacterium tuberculosisare complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-demand, near-patient technology, to simultaneously detect M. tuberculosisand rifampin resistance. Analytic tests of M. tuberculosisDNA demonstrated a limit of detection (LOD) of 4.5 genomes per reaction. Studies using sputum spiked with known numbers of M. tuberculosisCFU predicted a clinical LOD of 131 CFU/ml. Killing studies showed that the assay's buffer decreased M. tuberculosisviability by at least 8 logs, substantially reducing biohazards. Tests of 23 different commonly occurring rifampin resistance mutations demonstrated that all 23 (100%) would be identified as rifampin resistant. An analysis of 20 nontuberculosis mycobacteria species confirmed high assay specificity. A small clinical validation study of 107 clinical sputum samples from suspected tuberculosis cases in Vietnam detected 29/29 (100%) smear-positive culture-positive cases and 33/39 (84.6%) or 38/53 (71.7%) smear-negative culture-positive cases, as determined by growth on solid medium or on both solid and liquid media, respectively. M. tuberculosiswas not detected in 25/25 (100%) of the culture-negative samples. A study of 64 smear-positive culture-positive sputa from retreatment tuberculosis cases in Uganda detected 63/64 (98.4%) culture-positive cases and 9/9 (100%) cases of rifampin resistance. Rifampin resistance was excluded in 54/55 (98.2%) susceptible cases. Specificity rose to 100% after correcting for a conventional susceptibility test error. In conclusion, this highly sensitive and simple-to-use system can detect M. tuberculosisdirectly from sputum in less than 2 h.

Published
2010
Full Text
View/download PDF

33. Propionibacterium acnesTypes I and II Represent Phylogenetically Distinct Groups

Author

McDowell, Andrew, Valanne, Susanna, Ramage, Gordon, Tunney, Michael M., Glenn, Josephine V., McLorinan, Gregory C., Bhatia, Ajay, Maisonneuve, Jean-Francois, Lodes, Michael, Persing, David H., and Patrick, Sheila

Abstract

ABSTRACTAlthough two phenotypes of the opportunistic pathogen Propionibacterium acnes(types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for types I and II, respectively, we investigated the prevalences of the two types among 132 P. acnesisolates. Analysis of isolates from failed prosthetic hip implants (n= 40) revealed approximately equal numbers of type I and II organisms. Isolates from failed prosthetic hip-associated bone (n= 6) and tissue (n= 38) samples, as well as isolates from acne (n= 22), dental infections (n= 8), and skin removed during surgical incision (n= 18) were predominately of type I. A total of 11 (8%) isolates showed atypical MAb labeling and could not be conclusively identified. Phylogenetic analysis of P. acnesby nucleotide sequencing revealed the 16S rRNA gene to be highly conserved between types I and II. In contrast, sequence analysis of recAand a putative hemolysin gene (tly) revealed significantly greater type-specific polymorphisms that corresponded to phylogenetically distinct cluster groups. All 11 isolates with atypical MAb labeling were identified as type I by sequencing. Within the recAand tlyphylogenetic trees, nine of these isolates formed a cluster distinct from other type I organisms, suggesting a further phylogenetic subdivision within type I. Our study therefore demonstrates that the phenotypic differences between P. acnestypes I and II reflect deeper differences in their phylogeny. Furthermore, nucleotide sequencing provides an accurate method for identifying the type status of P. acnesisolates.

Published
2005
Full Text
View/download PDF

34. Isolation of a New Subspecies, Bartonella vinsoniisubsp. arupensis, from a Cattle Rancher: Identity with Isolates Found in Conjunction with Borrelia burgdorferiand Babesia microtiamong Naturally Infected Mice

Author

Welch, David F., Carroll, Karen C., Hofmeister, Erik K., Persing, David H., Robison, Denise A., Steigerwalt, Arnold G., and Brenner, Don J.

Abstract

ABSTRACTBacteremia with fever due to a novel subspecies of Bartonella vinsoniiwas found in a cattle rancher. The subspecies shared major characteristics of the genus Bartonellain terms of most biochemical features and cellular fatty acid profile, but it was distinguishable from other subspecies of B. vinsoniiby good growth on heart infusion agar supplemented with X factor and by its pattern of enzymatic hydrolysis of peptide substrates. DNA relatedness studies verified that the isolate belonged to the genusBartonellaand that it was genotypically related toB. vinsonii. The highest level of relatedness was observed with recently characterized strains from naturally infected mice that were coinfected with Borrelia burgdorferiand Babesia microti. We propose the name Bartonella vinsoniisubsp. arupensissubsp. nov. as the new subspecies to accommodate these human and murine isolates.

Published
1999
Full Text
View/download PDF

35. Clinical Evaluation of the Gen-Probe Amplified Direct Test for Detection of Mycobacterium tuberculosisComplex Organisms in Cerebrospinal Fluid

Author

Lang, Anne M., Feris-Iglesias, Jesus, Pena, Chabela, Sanchez, Jacqueline F., Stockman, Leslie, Rys, Paul, Roberts, Glenn D., Henry, Nancy K., Persing, David H., and Cockerill, Franklin R.

Abstract

ABSTRACTEighty-four cerebrospinal fluid (CSF) samples from different children who presented with signs and symptoms of meningitis were evaluated for the presence of Mycobacterium tuberculosiscomplex organisms by the Gen-Probe AmplifiedMycobacterium tuberculosisDirect Test (MTD; Gen-Probe, San Diego, Calif.). All CSF samples had negative acid-fast smears by the Ziehl-Neelsen staining method. M. tuberculosiswas recovered from five samples. M. tuberculosisdid not grow from 19 additional samples, but the samples were from patients who fulfilled specific clinical and laboratory criteria for probable tuberculous meningitis (TBM). The remaining samples (n= 60) were from patients with other infections or noninfectious causes of meningitis. The results of the MTD were interpreted as positive or negative on the basis of recommended cutoff values for respiratory specimens. These results were interpreted as true or false positives or true or false negatives on the basis of the results of M. tuberculosisculture or whether the patient fulfilled criteria for probable TBM. The Gen-Probe MTD was 33% sensitive and 100% specific for detecting M. tuberculosiscomplex organisms in these 84 CSF samples. If the cutoff values for positive results were decreased for the MTD (=11,000 versus =30,000 relative light units), the sensitivity increased to 83% and the specificity remained 100%. These results for the MTD are encouraging considering that TBM is a highly fatal disease and difficult to diagnose by conventional laboratory techniques.

Published
1998
Full Text
View/download PDF

36. Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2/Flu/RSV Test

Author

Mostafa, Heba H., Carroll, Karen C., Hicken, Rachel, Berry, Gregory J., Manji, Ryhana, Smith, Elizabeth, Rakeman, Jennifer L., Fowler, Randal C., Leelawong, Mindy, Butler-Wu, Susan M., Quintero, David, Umali-Wilcox, Minette, Kwiatkowski, Robert W., Persing, David H., Weir, Fred, and Loeffelholz, Michael J.

Abstract

With the approach of respiratory virus season in the Northern Hemisphere, clinical microbiology and public health laboratories will need rapid diagnostic assays to distinguish severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza virus and respiratory syncytial virus (RSV) infections for diagnosis and surveillance. In this study, the clinical performance of the Xpert Xpress SARS-CoV-2/Flu/RSV test (Cepheid, Sunnyvale, CA, USA) for nasopharyngeal swab specimens was evaluated in four centers: Johns Hopkins Medical Microbiology Laboratory, Northwell Health Laboratories, NYC Public Health Laboratory, and Los Angeles County/University of Southern California (LAC+USC) Medical Center.

Published
2020
Full Text
View/download PDF

37. Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV

Author

Södersten, Erik, Ongarello, Stefano, Mantsoki, Anna, Wyss, Romain, Persing, David H., Banderby, Sara, Strömqvist Meuzelaar, Linda, Prieto, Jacqueline, Gnanashanmugam, Devasena, Khatri, Purvesh, Schumacher, Samuel G., and Denkinger, Claudia M.

Abstract

A nonsputum triage test to rule out tuberculosis (TB) disease is a WHO high-priority diagnostic, and a combinatory score based on a 3-gene host signature has shown promise in discriminating TB from other illnesses. We evaluated the accuracy of an early-prototype cartridge assay (“Xpert MTB Host Response” or Xpert-MTB-HR-Prototype) of this 3-gene signature on biobanked blood samples from people living with HIV (PLHIV) against a comprehensive microbiological reference standard (CMRS) and against Xpert MTB/RIF on the first sputum sample alone.

Published
2020
Full Text
View/download PDF

40. Rapid Detection of Extensive Drug Resistance by Xpert MTB/XDR Optimizes Therapeutic Decision-Making in Rifampin-Resistant Tuberculosis Patients.

Author

Chen X, Li R, Ge S, Li Y, Cai C, Weng T, Zhang Y, Jiang J, Feng Z, Chen Y, Zhang Y, Ma J, Persing DH, Chen J, Tang YW, Sun F, and Zhang W

Subjects
Humans, Rifampin pharmacology, Rifampin therapeutic use, Sensitivity and Specificity, Fluoroquinolones pharmacology, Fluoroquinolones therapeutic use, Drug Resistance, Bacterial, Sputum microbiology, Antibiotics, Antitubercular pharmacology, Tuberculosis diagnosis, Mycobacterium tuberculosis genetics, Tuberculosis, Multidrug-Resistant diagnosis, Tuberculosis, Multidrug-Resistant drug therapy, Tuberculosis, Multidrug-Resistant microbiology
Abstract

The Xpert MTB/XDR assay met the critical need for etiologic diagnosis of tuberculosis and rifampin resistance in previous studies. However, its benefits in tailoring the treatment regimen and improving the outcome for patients with rifampin-resistant tuberculosis (RR-TB) require further investigation. In this study, the Xpert MTB/XDR assay was used to determine the resistance profile of second-line drugs for RR-TB patients in two registered multicenter clinical trials, TB-TRUST (NCT03867136) and TB-TRUST-plus (NCT04717908), with the aim of testing the efficacy of all-oral shorter regimens in RR-TB patients in China. Patients would receive the fluoroquinolone-based all-oral shorter regimen, the injectable-containing regimen, or the bedaquiline-based regimen depending on fluoroquinolone susceptibility by using Xpert MTB/XDR. Among the 497 patients performed with Xpert MTB/XDR, 128 (25.8%) had infections resistant to fluoroquinolones and/or second-line injectable drugs (SLIDs). A total of 371 participants were recruited for the trials, and whole-genome sequencing (WGS) was performed on all corresponding culture-positive baseline strains. Taking the WGS results as the standard, the accuracy of the Xpert MTB/XDR assay in terms of resistance detection was 95.2% to 99.0% for all drugs. A total of 33 cases had inconsistent results, 9 of which were due to resistance heterogeneity. Most of the patients (241/281, 85.8%) had sputum culture conversion at 2 months. In conclusion, the Xpert MTB/XDR assay has the potential to serve as a quick reflex test in patients with RR-TB, as detected via Xpert MTB/RIF, to provide a reliable drug susceptibility profile of the infecting Mycobacterium tuberculosis strain and to initiate optimized treatment promptly., Competing Interests: The authors declare no conflict of interest.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results