Search

Your search keyword '"Jolien Vermeire"' showing total 1 results
Start Over Author "Jolien Vermeire" Journal journal of clinical microbiology
1 results on '"Jolien Vermeire"'

1. Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

Author

Eva Malatinkova, Karen Vervisch, Pawel Bonczkowski, Bruno Verhasselt, Wim Trypsteen, Jolien Vermeire, Maja Kiselinova, Peter Messiaen, Ward De Spiegelaere, and Linos Vandekerckhove

Subjects
Microbiology (medical), viruses, Terminal Repeat Sequences, Human immunodeficiency virus (HIV), Biology, medicine.disease_cause, Polymerase Chain Reaction, Virology, DNA extraction, Long terminal repeat, law.invention, genomic DNA, Plasmid, Viral replication, law, DNA, Viral, HIV-1, medicine, Humans, Digital polymerase chain reaction, Polymerase chain reaction, Plasmids
Abstract

Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results