1. Genotyping of Clinical Herpes Simplex Virus Type 1 Isolates by Use of Restriction Enzymes
- Author
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Jan-Åke Liljeqvist, Tomas Bergström, and Peter Norberg
- Subjects
Microbiology (medical) ,Genotype ,viruses ,Herpesvirus 1, Human ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Virus ,law.invention ,Viral Envelope Proteins ,law ,Virology ,medicine ,Cluster Analysis ,Humans ,Typing ,Genotyping ,Polymerase chain reaction ,Genetics ,Herpes Simplex ,DNA Restriction Enzymes ,DNA Fingerprinting ,Restriction enzyme ,Herpes simplex virus ,DNA profiling ,DNA, Viral ,Polymorphism, Restriction Fragment Length - Abstract
Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.
- Published
- 2006