1. Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110
- Author
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Leonard N. Mukasa, Jonathan E. Golub, Laurence S. Magder, William H. Benjamin, Jafar H. Razeq, Nancy Hooper, Wendy A. Cronin, Monica J. Lathan, William R. Bishai, Nancy G. Baruch, and Donna Mulcahy
- Subjects
Microbiology (medical) ,Tuberculosis ,Epidemiologic study ,Population ,Gene Dosage ,Oligonucleotides ,Biology ,Microbiology ,Mycobacterium tuberculosis ,medicine ,Humans ,Typing ,education ,Tuberculosis, Pulmonary ,Aged ,education.field_of_study ,Transmission (medicine) ,Mycobacteriology and Aerobic Actinomycetes ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Virology ,DNA Fingerprinting ,respiratory tract diseases ,Bacterial Typing Techniques ,DNA profiling ,DNA Transposable Elements ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length - Abstract
Restriction fragment length polymorphism (RFLP) analysis of IS 6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis . However, low-copy (≤5) IS 6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS 6110 -spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS 6110 alone ( P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.
- Published
- 2001