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Your search keyword '"Christopher R. Braden"' showing total 2 results
Start Over Author "Christopher R. Braden" Journal journal of clinical microbiology
2 results on '"Christopher R. Braden"'

1. Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition

Author

B A Baiba Pironis, Jill Heckendorf, Volker Mai, Jon Mark Hirshon, and Christopher R. Braden

Subjects
Microbiology (medical), DNA, Bacterial, Diarrhea, Surveillance study, Nucleic Acid Denaturation, Asymptomatic, DNA, Ribosomal, Polymerase Chain Reaction, Microbiology, Feces, fluids and secretions, RNA, Ribosomal, 16S, medicine, Humans, RNA RIBOSOMAL 16S, biology, Bacteria, Genes, rRNA, Bacteriology, Biodiversity, biology.organism_classification, DNA Fingerprinting, Intestines, DNA profiling, Electrophoresis, Polyacrylamide Gel, medicine.symptom, Temperature gradient gel electrophoresis
Abstract

We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity and strong bands indicating the overgrowth of selected bacteria or bacterial groups.

Published
2006

2. Molecular and conventional epidemiology of Mycobacterium tuberculosis in Botswana: a population-based prospective study of 301 pulmonary tuberculosis patients

Author

M. Mwasekaga, Thomas A. Kenyon, Faustina Montsho, Jordan W. Tappero, Christopher R. Braden, Moeti Tl, Matlhatso Kesupile-Reed, Nancy J. Binkin, Jeffery D. Sheppard, Shahin Lockman, Colette Hirschfeldt, Charles L. Woodley, Michael Steinman, and Malebogo Notha

Subjects
Microbiology (medical), Adult, Male, medicine.medical_specialty, Tuberculosis, Population, Mycobacterium tuberculosis, Acquired immunodeficiency syndrome (AIDS), Internal medicine, Genotype, medicine, Humans, Prospective Studies, education, Letters to the Editor, Tuberculosis, Pulmonary, education.field_of_study, Molecular Epidemiology, Botswana, biology, Molecular epidemiology, AIDS-Related Opportunistic Infections, Mycobacteriology and Aerobic Actinomycetes, biology.organism_classification, medicine.disease, Virology, DNA Fingerprinting, DNA profiling, Population Surveillance, DNA Transposable Elements, Female, Restriction fragment length polymorphism
Abstract

Little is known about patterns of tuberculosis (TB) transmission among populations in developing countries with high rates of TB and human immunodeficiency virus (HIV) infection. To examine patterns of TB transmission in such a setting, we performed a population-based DNA fingerprinting study among TB patients in Botswana. Between January 1997 and July 1998, TB patients from four communities in Botswana were interviewed and offered HIV testing. Their Mycobacterium tuberculosis isolates underwent DNA fingerprinting using IS 6110 restriction fragment length polymorphism, and those with matching fingerprints were reinterviewed. DNA fingerprints with >5 bands were considered clustered if they were either identical or differed by at most one band, while DNA fingerprints with ≤5 bands were considered clustered only if they were identical. TB isolates of 125 (42%) of the 301 patients with completed interviews and DNA fingerprints fell into 20 different clusters of 2 to 16 patients. HIV status was not associated with clustering. Prior imprisonment was the only statistically significant risk factor for clustering (risk ratio, 1.5; 95% confidence interval, 1.1 to 2.0). In three communities where the majority of eligible patients were enrolled, 26 (11%) of 243 patients overall and 26 (25%) of 104 clustered patients shared both a DNA fingerprint and strong antecedent epidemiologic link. Most of the increasing TB burden in Botswana may be attributable to reactivation of latent infection, but steps should be taken to control ongoing transmission in congregate settings. DNA fingerprinting helps determine loci of TB transmission in the community.

Published
2001

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