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Your search keyword '"Besser, John"' showing total 7 results
Start Over Author "Besser, John" Journal journal of clinical microbiology
7 results on '"Besser, John"'

1. Clonal Distribution of Invasive Pneumococcal Isolates from Children and Selected Adults in the United States Prior to 7-Valent Conjugate Vaccine Introduction

Author

Gertz, Robert E., primary, McEllistrem, M. Catherine, additional, Boxrud, David J., additional, Li, Zhongya, additional, Sakota, Varja, additional, Thompson, Terry A., additional, Facklam, Richard R., additional, Besser, John M., additional, Harrison, Lee H., additional, Whitney, Cynthia G., additional, and Beall, Bernard, additional

Published
2005
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3. Clonal Distribution of Invasive Pneumococcal Isolatesfrom Children and Selected Adults in the United States Prior to7-Valent Conjugate VaccineIntroduction

Author

Gertz, Robert E., primary, McEllistrem, M. Catherine, additional, Boxrud, David J., additional, Li, Zhongya, additional, Sakota, Varja, additional, Thompson, Terry A., additional, Facklam, Richard R., additional, Besser, John M., additional, Harrison, Lee H., additional, Whitney, Cynthia G., additional, and Beall, Bernard, additional

Published
2003
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5. Evaluation of Pulsed-Field Gel Electrophoresis in Epidemiological Investigations of Meningococcal Disease Outbreaks Caused by Neisseria meningitidis Serogroup C

Author

Popovic, Tanja, primary, Schmink, Susanna, additional, Rosenstein, Nancy A., additional, Ajello, Gloria W., additional, Reeves, Michael W., additional, Plikaytis, Brian, additional, Hunter, Susan B., additional, Ribot, Efrain M., additional, Boxrud, David, additional, Tondella, Maria L., additional, Kim, Chung, additional, Noble, Corie, additional, Mothershed, Elizabeth, additional, Besser, John, additional, and Perkins, Bradley A., additional

Published
2001
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6. Evaluation of Pulsed-Field Gel Electrophoresis in Epidemiological Investigations of Meningococcal Disease Outbreaks Caused byNeisseria meningitidisSerogroup C

Author

Popovic, Tanja, Schmink, Susanna, Rosenstein, Nancy A., Ajello, Gloria W., Reeves, Michael W., Plikaytis, Brian, Hunter, Susan B., Ribot, Efrain M., Boxrud, David, Tondella, Maria L., Kim, Chung, Noble, Corie, Mothershed, Elizabeth, Besser, John, and Perkins, Bradley A.

Abstract

ABSTRACTSince 1990, the frequency of Neisseria meningitidisserogroup C (NMSC) outbreaks in the United States has increased. Based on multilocus enzyme electrophoresis (MEE), the current molecular subtyping standard, most of the NMSC outbreaks have been caused by isolates of several closely related electrophoretic types (ETs) within the ET-37 complex. We chose 66 isolates from four well-described NMSC outbreaks that occurred in the United States from 1993 to 1995 to evaluate the potential of pulsed-field gel electrophoresis (PFGE) to identify outbreak-related isolates specific for each of the four outbreaks and to differentiate between them and 50 sporadic isolates collected during the outbreak investigations or through active laboratory-based surveillance from 1989 to 1996. We tested all isolates collected during the outbreak investigations by four other molecular subtyping methods: MEE, ribotyping (ClaI), random amplified polymorphic DNA assay (two primers), and serotyping and serosubtyping. Among the 116 isolates, we observed 11 clusters of 39 NheI PFGE patterns. Excellent correlation between the PFGE and the epidemiological data was observed, with an overall sensitivity of 85% and specificity of 71% at the 95% pattern relatedness breakpoint using either 1.5 or 1.0% tolerance. For all four analyzed outbreaks, PFGE would have given public health officials additional support in declaring an outbreak and making appropriate public health decisions.

Published
2001
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7. Clonal distribution of invasive pneumococcal isolates from children and selected adults in the United States prior to 7-valent conjugate vaccine introduction.

Author

Gertz RE Jr, McEllistrem MC, Boxrud DJ, Li Z, Sakota V, Thompson TA, Facklam RR, Besser JM, Harrison LH, Whitney CG, and Beall B

Subjects
Adult, Alleles, Child, Electrophoresis, Gel, Pulsed-Field, Genotype, Humans, Penicillin Resistance, Serotyping, Streptococcus pneumoniae genetics, Streptococcus pneumoniae immunology, Vaccination, Vaccines, Conjugate immunology, Pneumococcal Vaccines immunology, Streptococcus pneumoniae classification
Abstract

Theseven-valent pneumococcal conjugated polysaccharide vaccine PC7V was licensed for use among children in 2000. Since 90 serotypes of pneumococci exist, an increase in nonvaccine serotypes could occur through immune selection for capsular type switching. Eleven hundred sixty-eight invasive isolates (24 serotypes), recovered primarily from pediatric patients (855 isolates = 73%) and 22 reference strains of known multilocus sequence types (STs) were subjected to macrorestriction profiling (pulsed-field gel electrophoresis [PFGE]). The correlation of 187 ST results (including 49 newly discovered STs) with the PFGE data assigned 1,042 (89.2%) study isolates to 46 defined clonal complexes or genetic lineages based on related multilocus STs (BURST). Seventeen clonal complexes were represented by 2 to 10 related allelic profiles (STs), while 33 lineages (including reference strains) consisted of single STs with 4 or fewer allelic identities to other STs found in the study. Expansion of the BURST analysis to a global analysis of all known pneumococcal STs (as of 27 November 2002) reduced the number of single ST lineages from 33 to 8, and the number of multi-ST clonal complexes was reduced from 17 to 13. In this work, we established the basic genetic structure within individual serotypes prior to PC7V use. The resultant database will be useful for detecting potential selective effects of this vaccine in postvaccine surveillance.

Published
2003
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