Search

Your search keyword '"Blanchet, Odile"' showing total 3 results
Start Over Author "Blanchet, Odile" Journal journal of clinical medicine
3 results on '"Blanchet, Odile"'

1. A Tear Metabolomic Profile Showing Increased Ornithine Decarboxylase Activity and Spermine Synthesis in Thyroid-Associated Orbitopathy

Author

Billiet, Benjamin, de la Barca, Juan Manuel Chao, Ferré, Marc, Muller, Jeanne, Vautier, Anaïs, Assad, Sophie, Blanchet, Odile, Tessier, Lydie, Wetterwald, Céline, Faure, Justine, Urbanski, Geoffrey, Simard, Gilles, Mirebeau-Prunier, Delphine, Rodien, Patrice, Gohier, Philippe, and Reynier, Pascal

Subjects
Clinical Research, Prevention, Aetiology, 2.1 Biological and endogenous factors, Metabolic and endocrine, Graves' ophthalmopathy, metabolomics, ornithine decarboxylase, spermine, thyroid-associated orbitopathy, Graves’ ophthalmopathy, Clinical Sciences
Abstract

About half of patients with Graves' disease develop an orbitopathy related to an inflammatory expansion of the periorbital adipose tissue and muscles. We used a targeted metabolomic approach measuring 188 metabolites by mass spectrometry to compare the metabolic composition of tears in patients with active (n = 21) versus inactive (n = 24) thyroid-associated orbitopathy. Among the 44 metabolites accurately measured, 8 showed a significant alteration of their concentrations between the two groups. Two short-chain acylcarnitines, propionylcarnitine and butyrylcarnitine, and spermine showed increased concentrations in the tears of patients with active orbitopathy, whereas ornithine, glycine, serine, citrulline and histidine showed decreased concentrations in this group. In addition, the ratio putrescine/ornithine, representing the activity of ornithine decarboxylase, was significantly increased in patients with active compared to inactive orbitopathy (p = 0.0011, fold change 3.75). The specificity of this candidate biomarker was maintained when compared to a control group with unclassified dry eye disease. Our results suggest that the stimulation of ornithine decarboxylase by TSH receptor autoantibodies in orbital fibroblasts could lead to increased synthesis of spermine, through the increased activity of ornithine decarboxylase, that may contribute to periorbital expansion in Graves' ophthalmopathy.

Published
2022

2. A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies

Author

Chao de la Barca, Juan M., primary, Rondet-Courbis, Barnabé, additional, Ferré, Marc, additional, Muller, Jeanne, additional, Buisset, Adrien, additional, Leruez, Stéphanie, additional, Plubeau, Guillaume, additional, Macé, Thibaut, additional, Moureauzeau, Laurie, additional, Chupin, Stéphanie, additional, Tessier, Lydie, additional, Blanchet, Odile, additional, Lenaers, Guy, additional, Procaccio, Vincent, additional, Mirebeau-Prunier, Delphine, additional, Simard, Gilles, additional, Gohier, Philippe, additional, Miléa, Dan, additional, and Reynier, Pascal, additional

Published
2020
Full Text
View/download PDF

3. CD45RC Expression of Circulating CD8+ T Cells Predicts Acute Allograft Rejection: A Cohort Study of 128 Kidney Transplant Patients

Author

Lemerle, Marie, Garnier, Anne-Sophie, Planchais, Martin, Brilland, Benoit, Delneste, Yves, Subra, Jean-François, Blanchet, Odile, Blanchard, Simon, Croue, Anne, Duveau, Agnès, Augusto, Jean-François, Département de Néphrologie-Dialyse-Transplantation [CHU Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Innate Immunity and Immunotherapy (CRCINA-ÉQUIPE 7), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Laboratoire d'Immunologie et d'Allergologie [CHU Angers], Centre de Ressources Biologiques [CHU d'Angers] (CRB CHU d'Angers BB-0033-00038), Département de Pathologie Cellulaire et Tissulaire [CHU Angers] (Laboratoire d’Histopathologie-Cytopathologie), Université d'Angers (UA)-Centre Hospitalier Universitaire d'Angers (CHU Angers), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), and Bernardo, Elizabeth

Subjects
medicine.medical_specialty, Lymphocyte, T cell, lcsh:Medicine, kidney transplantation, [SDV.CAN]Life Sciences [q-bio]/Cancer, Human leukocyte antigen, 030230 surgery, lymphocyte, Gastroenterology, acute rejection, Article, 03 medical and health sciences, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Antigen, Internal medicine, Medicine, Cytotoxic T cell, Kidney transplantation, CD45RC, 030304 developmental biology, 0303 health sciences, business.industry, lcsh:R, General Medicine, medicine.disease, 3. Good health, body regions, Transplantation, medicine.anatomical_structure, business, CD8
Abstract

Predictive biomarkers of acute rejection (AR) are lacking. Pre-transplant expression of CD45RC on blood CD8+ T cells has been shown to predict AR in kidney transplant (KT) patients. The objective of the present study was to study CD45RC expression in a large cohort of KT recipients exposed to modern immunosuppressive regimens. CD45RC expression on T cells was analyzed in 128 KT patients, where 31 patients developed AR, of which 24 were found to be T-cell mediated (TCMR). Pre-transplant CD4+ and CD8+ CR45RChigh T cell proportions were significantly higher in patients with AR. The frequency of CD45RChigh T cells was significantly associated with age at transplantation but was not significantly different according to gender, history of transplantation, pre-transplant immunization, and de novo donor specific anti-Human Leucocyte Antigen (HLA) antibody. Survival-free AR was significantly better in patients with CD8+ CD45RChigh T cells below 58.4% (p = 0.0005), but not different according to CD4+ T cells (p = 0.073). According to multivariate analysis, CD8+ CD45RChigh T cells above 58.4% increased the risk of AR 4-fold (HR 3.96, p = 0.003). Thus, pre-transplant CD45RC expression on CD8+ T cells predicted AR, mainly TCMR, in KT patients under modern immunosuppressive therapies. We suggest that CD45RC expression should be evaluated in a prospective study to validate its usefulness to quantify the pre-transplant risk of AR.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results