1. Evaluation of DPC immulite 2000 Toxoplasma quantitative IgG/IgM kits for automated toxoplasmosis serology with immulite 2000
- Author
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Florence Robert-Gangneux, Chantal Bourhis, Sylviane Chevrier, Jean-Pierre Gangneux, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Service de Parasitologie-Mycologie [Rennes], Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université de Rennes (UR), and Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
- Subjects
TORCH screening ,Clinical Biochemistry ,MESH: Reagent Kits, Diagnostic ,Antibodies, Protozoan ,MESH: Titrimetry ,Serology ,Automation ,0302 clinical medicine ,MESH: Automation ,antibody ,Immunology and Allergy ,Statistical analysis ,030212 general & internal medicine ,MESH: Immunoglobulin G ,Immunoassay ,0303 health sciences ,biology ,medicine.diagnostic_test ,MESH: Toxoplasma ,Titrimetry ,Hematology ,MESH: Infant ,Congenital toxoplasmosis ,MESH: Immunoglobulin M ,3. Good health ,Medical Laboratory Technology ,Titer ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,MESH: Toxoplasmosis ,ELISA ,Female ,Antibody ,MESH: Immunoassay ,Toxoplasma ,Toxoplasmosis ,Microbiology (medical) ,Sensitivity and Specificity ,03 medical and health sciences ,medicine ,MESH: Antibodies, Protozoan ,Humans ,Seroconversion ,MESH: Humans ,030306 microbiology ,business.industry ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Infant ,Original Articles ,medicine.disease ,MESH: Sensitivity and Specificity ,Immunoglobulin M ,Immunoglobulin G ,Immunology ,biology.protein ,Reagent Kits, Diagnostic ,business ,MESH: Female ,serological diagnosis - Abstract
In this study, we evaluated a new immunoassay for the automated detection of anti‐toxoplasma IgG and IgM with Immulite 2000(®) (DPC‐Siemens, La Garenne‐Colombes, France). We tested 280 sera from 112 patients with past infection (PI), 40 PI with residual IgM, 75 seronegatives, 16 infants (31 sera) monitored for neonatal screening of congenital toxoplasmosis, and 13 patients with recent seroconversion (SC) (22 sequential sera). Detection sensitivity and specificity for IgG were 99 and 100%, respectively. IgG titers obtained with Immulite 2000(®) were higher than with Vidas(®) (BioMérieux, Marcy I'Etoile, France) and Access(®) (Beckman‐Coulter, Villepinte, France) (paired Wilcoxon test z=4.44 and z=3.67, respectively, P
- Published
- 2009
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