1. Induction of pathogenic anti-dsDNA antibodies is controlled on the level of B cells in a non-lupus prone mouse strain.
- Author
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Langnickel D, Enghard P, Klein C, Undeutsch R, Hocher B, Manz R, Burmester GR, and Riemekasten G
- Subjects
- Animals, Female, Immunization, Lupus Vulgaris immunology, Mice, Mice, Inbred BALB C, Mice, Inbred NZB, Peptide Fragments immunology, T-Lymphocytes immunology, snRNP Core Proteins, Antibodies, Antinuclear biosynthesis, B-Lymphocytes immunology, DNA immunology, Lupus Vulgaris genetics, Ribonucleoproteins, Small Nuclear immunology
- Abstract
The SmD1(83-119) peptide is a main target of autoantibodies and T cells in human and murine lupus, but its role in autoimmunity induction remains elusive. Therefore, female Balb/c mice and (NZW x Balb/c)F1 [CWF1] mice with identical MHC haplotype as lupus prone NZB/W mice were immunized with SmD1(83-119). Immunizations of CWF1 mice with SmD1(83-119), but not with the controls (irrelevant peptide, HEL peptide, or saline), induced anti-SmD1(83-119) and anti-dsDNA antibodies and proteinuria not present in Balb/c mice. DsDNA-specific plasma cell induction after SmD1(83-119) immunizations was confirmed by ELISPOT assays showing that the generation of dsDNA-specific antibody forming cells (AFC) was mainly driven by increased T-cell help. T-cell help for the generation of dsDNA-specific AFC was also present in saline-treated CWF1 mice but was controlled on the levels of B cells preventing autoimmunity.
- Published
- 2006
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