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Nitrogen (N) stabilizers such as urease inhibitors (UI) and nitrification inhibitors (NI) are promising tools to mitigate N losses from fertilized soils. To better understand the efficacy of UI and NI, a 2-year field trial was carried out in the North China Plain (NCP) to analyze the impact of UI and NI on NH 3 loss and N transformations following urea fertilizer application. Additionally, a laboratory study using an automated continuous-flow incubation system was conducted under controlled conditions, using the same soil, to evaluate the mitigation potential of the N stabilizers on NO, N 2 O and CO 2 emission. The results showed that a novel UI product, Limus® (BASF, Germany), containing active ingredients N-(n-butyl) thiophosphoric triamide (NBPT) and N-(n-propyl) thiophosphoric triamide (NPPT), limited ammonium formation rate and reduced NH 3 loss in both maize (by 85 and 96% for year 1 and 2, respectively) and wheat (by 41 and 64%, for year 1 and 2, respectively) seasons. The 3, 4-dimethypyrazole phosphate (DMPP) amendment significantly retarded nitrate formation rate but the effectiveness was compromised by high ambient temperature; additionally, the NI amendment increased NH 3 emission (by 16 and 24% for maize and wheat year 2, respectively). In the laboratory incubation experiment, addition of the UI reduced emissions of NO, N 2 O and CO 2 by 89, 73 and 19%, respectively, while the addition of the NI reduced the respective emissions by 95, 83 and 24% over the 16 d incubation period. These results suggest that N stabilizers can play a significant role in the mitigation of the environmental impacts of intensive agriculture, and, in particular, that the UI amendment may have the better potential to reduce N losses in the climatic and soil conditions of the NCP, and that the NI should be avoided under high ambient temperatures. Image 1 • Urease inhibitor amendment of urea reduced NH 3 , NO and N 2 O emissions. • Nitrification inhibitor amendment of urea reduced NO and N 2 O but increased NH 3 emissions. • Efficacy of the nitrification inhibitor was compromised by high ambient temperature. • Urease and nitrification inhibitor amendment of urea reduced CO 2 emissions. [ABSTRACT FROM AUTHOR]