1. Fast chromatographic separation for the quantitation of the main flavone dyes in Reseda luteola (weld)
- Author
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Goverdina C. H. Derksen, Teris A. van Beek, Eric Simon Gabriel Maria Mattheussens, Alexandre Villela, Han Zuilhof, and Elbert J.C. van der Klift
- Subjects
Reseda luteola ,Sensitivity and Specificity ,Biochemistry ,Flavones ,High-performance liquid chromatography ,Statistics, Nonparametric ,l ,Analytical Chemistry ,chemistry.chemical_compound ,bilobalide ,Glucosides ,Maceration (wine) ,Resedaceae ,diode-array detection ,Coloring Agents ,Chromatography, High Pressure Liquid ,VLAG ,chemistry.chemical_classification ,Detection limit ,performance liquid-chromatography ,Chromatography ,biology ,Plant Extracts ,Chemistry ,Methanol ,Extraction (chemistry) ,Organic Chemistry ,Reproducibility of Results ,General Medicine ,biology.organism_classification ,portugal ,Organische Chemie ,quantification ,Textile Industry ,Linear Models ,identification ,Luteolin ,Natural dye - Abstract
In the past decades, there has been a renewed interest in the use of natural dye plants for textile dyeing, e.g. Reseda luteola (weld). Its main yellow dye constituents are the flavones luteolin-7,3'-O-diglucoside, luteolin-7-O-glucoside and luteolin. The aim of this work was to develop a simple validated industrially usable quantitative method to assess the flavone content of R. luteola samples. The flavones were overnight extracted from the dried and ground aerial parts of the plant at room temperature via maceration with methanol-water 8:2. Afterwards, they were quantified through internal standardisation against chrysin by RP-HPLC-UV at 345 nm. The efficiency of the one-step extraction was 95%. The limits of detection (LOD) and quantitation (LOQ) were ≤ 1 ng and ≤ 3 ng, respectively, providing ample sensitivity for the purpose. The precision expressed as relative standard deviation of the entire method was
- Published
- 2011
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