1. Selective electromembrane extraction at low voltages based on analyte polarity and charge.
- Author
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Domínguez NC, Gjelstad A, Nadal AM, Jensen H, Petersen NJ, Hansen SH, Rasmussen KE, and Pedersen-Bjergaard S
- Subjects
- Animals, Electrodes, Electrophoresis, Capillary, Equipment Design, Ethers chemistry, Humans, Milk chemistry, Pharmaceutical Preparations blood, Pharmaceutical Preparations urine, Sensitivity and Specificity, Chemical Fractionation instrumentation, Electrochemical Techniques instrumentation, Membranes, Artificial, Pharmaceutical Preparations isolation & purification
- Abstract
Electromembrane extraction (EME) at low voltage (0-15 V) of 29 different basic model drug substances was investigated. The drug substances with logP<2.3 were not extracted at voltages less than 15 V. Extraction of drug substances with logP≥2.3 and with two basic groups were also effectively suppressed by the SLM at voltages less than 15 V. Drug substances with logP≥2.3 and with one basic group were all extracted at low voltages and with a strong compound selectivity which appeared to have some influence from the polar surface area of the compound. For this group of substances, recoveries varied between 0 and 23% at 5 V, whereas, recoveries varied between 5.5 and 51% at 15 V. Based on mass transfer differences related to charge, polarity, and polar surface, highly selective extractions of drug substances were demonstrated from human plasma, urine, and breast milk. An initial evaluation at low voltage (5 V) was compared with similar extractions at a more normal voltage level (50 V), and this supported that reliable data can be obtained under these low-voltage (mild) conditions by EME., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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