1. Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry
- Author
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Michelle L. Colgrave, Alun Jones, and PG Allingham
- Subjects
Proline ,Mass spectrometry ,Biochemistry ,High-performance liquid chromatography ,Mass Spectrometry ,Analytical Chemistry ,chemistry.chemical_compound ,Hydroxyproline ,Animals ,Derivatization ,chemistry.chemical_classification ,Sheep ,Chromatography ,Muscles ,Organic Chemistry ,Selected reaction monitoring ,Reproducibility of Results ,General Medicine ,Orders of magnitude (mass) ,Rats ,Amino acid ,chemistry ,Calibration ,Collagen ,Quantitative analysis (chemistry) - Abstract
Collagens are highly abundant mammalian proteins that contain a high content of hydroxylated amino acids such as hydroxyproline. We have exploited the high hydroxyproline content of collagen and developed a method for hydroxyproline quantification as a measure of collagen content in muscle samples. The novel method utilizes a highly selective and sensitive method of multiple reaction monitoring (MRM) by mass spectrometry. The analytical method is simple, rapid (5min), convenient (no derivatization), precise (17% RSD), accurate (90-108%), sensitive (4.88nmol/L) and linear (R(2)0.999) over three orders of magnitude (5-5000nmol/L).
- Published
- 2008
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