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A micro packed-column anion chromatograph with a UV detector has been constructed and the applicability of the system is demonstrated. A flexible fusedsilica capillary (0.19 mm I.D.) was used as a chromatographic tube and a surface-agglomerated and bonded low ion-exchange capacity anion-exchange resin, developed for ion chromatography, was packed into an ion-exchange column. Separations of inorganic ions, nucleobases, nucleotides and organic-acids were achieved using this system.

Anion-exchange chromatography of carboxylic acids and keto acids was carried out on an ion chromatograph equipped with a hollow-fibre ion-exchange membrane suppressor and a dual detector system comprised of a UV and a conductivity detector. Low-molecular-weight monocarboxylic acids were separated with a sodium tetraborate solution as eluent, and dicarboxylic acids and corresponding keto acids with a carbonate butter as eluent. The sensitivity of the conductivity detector to carboxylic acids was a function of the pK values of the acids. The detection limit for succinic acid was less than 0.5 nmol.