1. Parallel chiral sub/supercritical fluid chromatography screening as a framework for accelerated purification of pharmaceutical targets.
- Author
-
Losacco GL, DaSilva JO, Haidar Ahmad IA, Mangion I, Berger TA, and Regalado EL
- Subjects
- Indicators and Reagents, Pharmaceutical Preparations, Stereoisomerism, Chromatography, Supercritical Fluid methods
- Abstract
Chiral sub/supercritical fluid chromatography (SFC) has established itself as one of the preferred techniques for enantioseparations at both analytical and preparative scale. Herein, we introduce a parallel multicolumn SFC screening for automated chiral method development in fast-paced settings. The practicality and speed advantages of this approach are illustrated with parallel screening of a diverse set of chiral molecules across ten columns with five different organic modifiers/CO
2 based eluents enabling rapid identification of suitable enantioseparation conditions for accelerated purification of pharmaceutical targets. Rapid delivery turnarounds of pure enantiomers of less than 1 h from screening to target isolation are demonstrated illustrating the power of this approach., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier B.V.)- Published
- 2022
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