1. Isolation of secondary metabolites from Hortia oreadica (Rutaceae) leaves through high-speed counter-current chromatography.
- Author
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Severino VG, Cazal Cde M, Forim MR, da Silva MF, Rodrigues-Filho E, Fernandes JB, and Vieira PC
- Subjects
- Animals, Cell Line, Tumor, Cell Survival drug effects, Cinnamates isolation & purification, Cinnamates pharmacology, Humans, Indole Alkaloids isolation & purification, Indole Alkaloids pharmacology, Inhibitory Concentration 50, Limonins isolation & purification, Limonins pharmacology, Plasmodium falciparum drug effects, Quinazolines isolation & purification, Quinazolines pharmacology, Quinolines isolation & purification, Quinolines pharmacology, Rutaceae metabolism, Trypanosoma brucei rhodesiense drug effects, Countercurrent Distribution methods, Plant Extracts chemistry, Plant Leaves chemistry, Rutaceae chemistry
- Abstract
High-speed counter-current chromatography (HSCCC) with a two-phase solvent system (hexane-ethanol-acetonitrile-water 10:8:1:1, v/v) was applied to examine the leaves of Hortia oreadica, which afforded the known limonoid guyanin (1), the alkaloids rutaecarpin (2) and dictamnine (6), the dihydrocinnamic acid derivatives methyl 5,7-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoate (3), 5,8-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoic acid (4), together with the new E-3,4-dimethoxy-alpha(3-hydroxy-4-carbomethoxyphenyl)cinnamic acid (5). The recovery of compounds 1-6 was determined by comparison with LC-atmospheric pressure chemical ionization MS/MS data: 66.2%, 93.1%, 102.5%, 101.2%, 99.0% and 84.9%, respectively. Compound 3 showed IC(50) of 23.6microM against Plasmodium falciparum and 15.6microM against Trypanosoma brucei rhodesienses and was not toxic to KB cells (IC(50)>100microM).
- Published
- 2009
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