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Start Over Author "Medici, Nicola" Journal journal of cellular physiology
3 results on '"Medici, Nicola"'

1. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines

Author

Abbondanza, Ciro, primary, De Rosa, Caterina, additional, D'Arcangelo, Andrea, additional, Pacifico, Marianna, additional, Spizuoco, Clorinda, additional, Piluso, Giulio, additional, Di Zazzo, Erika, additional, Gazzerro, Patrizia, additional, Medici, Nicola, additional, Moncharmont, Bruno, additional, and Alfredo Puca, Giovanni, additional

Published
2011
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2. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines.

Author

Abbondanza, Ciro, De Rosa, Caterina, D'Arcangelo, Andrea, Pacifico, Marianna, Spizuoco, Clorinda, Piluso, Giulio, Di Zazzo, Erika, Gazzerro, Patrizia, Medici, Nicola, Moncharmont, Bruno, and Alfredo Puca, Giovanni

Subjects
GENETICS of breast cancer, ESTROGEN, GENE enhancers, PROMOTERS (Genetics), CELL lines, CANCER cells, RETINOBLASTOMA gene
Abstract

The retinoblastoma protein-interacting zinc-finger ( RIZ) gene, also known as PRDM2, encodes two protein products, RIZ1 and RIZ2, differing for the presence of a 202 aa domain, called PR domain, at the N-terminus of the RIZ1 molecule. While the histone H3 K9 methyltransferase activity of RIZ1 is associated with the negative control of cell proliferation, no information is currently available on either expression regulation of the RIZ2 form or on its biological activity. RIZ proteins act as ER co-activators and promote optimal estrogen response in female reproductive tissues. In estrogen-responsive cells, 17-β estradiol modulates RIZ gene expression producing a shift in the balanced expression of the two forms. Here, we demonstrate that an estrogen-responsive element (ERE) within the RIZ promoter 2 is regulated in a ligand-specific manner by ERα, through both the AF1 and AF2 domains. The pattern of ERα binding, histone H4 acetylation, and histone H3 cyclical methylation of lysine 9 was comparable to other estrogen-regulated promoters. Association of topoisomerase IIβ with the RIZ promoter 2 confirmed the transcriptional activation induced by estrogen. We hypothesize that RIZ2, acting as a negative regulator of RIZ1 function, mediates the proliferative effect of estrogen through regulation of survival and differentiation gene expression. J. Cell. Physiol. 227: 964-975, 2012. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2012
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3. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines

Author

Giulio Piluso, Patrizia Gazzerro, Caterina De Rosa, Marianna Pacifico, Nicola Medici, Bruno Moncharmont, Ciro Abbondanza, Giovanni Alfredo Puca, Erika Di Zazzo, Clorinda Spizuoco, Andrea D'Arcangelo, Abbondanza, Ciro, De Rosa, C, D'Arcangelo, A, Pacifico, M, Spizuoco, C, Piluso, Giulio, Di Zazzo, E, Gazzerro, P, Medici, Nicola, Moncharmont, B, and Puca, Ga

Subjects
Physiology, Cell Survival, Cellular differentiation, Clinical Biochemistry, Molecular Sequence Data, Breast Neoplasms, Biology, Histone H4, Histone H3, Cell Line, Tumor, Gene expression, Chlorocebus aethiops, Animals, Humans, Enhancer, Promoter Regions, Genetic, Transcription factor, Regulation of gene expression, Base Sequence, Estradiol, Nuclear Proteins, Promoter, Cell Differentiation, Cell Biology, Histone-Lysine N-Methyltransferase, Molecular biology, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Enhancer Elements, Genetic, COS Cells, Female, Transcription Factors
Abstract

""The retinoblastoma protein-interacting zinc-finger (RIZ) gene, also known as PRDM2, encodes two protein products, RIZ1 and RIZ2, differing for the presence of a 202 aa domain, called PR domain, at the N-terminus of the RIZ1 molecule. While the histone H3 K9 methyltransferase activity of RIZ1 is associated with the negative control of cell proliferation, no information is currently available on either expression regulation of the RIZ2 form or on its biological activity. RIZ proteins act as ER co-activators and promote optimal estrogen response in female reproductive tissues. In estrogen-responsive cells, 17-beta estradiol modulates RIZ gene expression producing a shift in the balanced expression of the two forms. Here, we demonstrate that an estrogen-responsive element (ERE) within the RIZ promoter 2 is regulated in a ligand-specific manner by ERa, through both the AF1 and AF2 domains. The pattern of ERa binding, histone H4 acetylation, and histone H3 cyclical methylation of lysine 9 was comparable to other estrogen-regulated promoters. Association of topoisomerase II beta with the RIZ promoter 2 confirmed the transcriptional activation induced by estrogen. We hypothesize that RIZ2, acting as a negative regulator of RIZ1 function, mediates the proliferative effect of estrogen through regulation of survival and differentiation gene expression. J. Cell. Physiol. 227: 964975, 2012. (C) 2011 Wiley Periodicals, Inc.""

Published
2011

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