1. Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells
- Author
-
Ruy Gastaldoni Jaeger, Vanessa Morais Freitas, Hernandes F. Carvalho, Livia Caires-Dos-Santos, Taize Machado Augusto, Adriane S. Siqueira, Karen S. P. Cruz, Basilio Smuczek, Suély V. Silva, and José Alexandre Marzagão Barbuto
- Subjects
Male ,0301 basic medicine ,Physiology ,Clinical Biochemistry ,Peptide ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,DU145 ,Cell Movement ,Laminin ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,medicine.diagnostic_test ,NEOPLASIAS PROSTÁTICAS ,Prostatic Neoplasms ,food and beverages ,Cell Biology ,Cell biology ,Adaptor Proteins, Vesicular Transport ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Podosomes ,Proteolysis ,Cancer cell ,Invadopodia ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Reactive Oxygen Species ,Cortactin - Abstract
Laminin peptides influence cancer biology. We investigated the role of a laminin-derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane-type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16-treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity.
- Published
- 2019
- Full Text
- View/download PDF