1. MicroRNA-30e-5p promotes cell growth by targeting PTPN13 and indicates poor survival and recurrence in lung adenocarcinoma.
- Author
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Zhuang L, Shou T, Li K, Gao CL, Duan LC, Fang LZ, Zhang QY, Chen ZN, Zhang C, Yang ST, and Li GF
- Subjects
- Adenocarcinoma diagnosis, Adenocarcinoma metabolism, Adenocarcinoma mortality, Adenocarcinoma of Lung, Aged, Animals, Cell Line, Tumor, Cell Survival, ErbB Receptors genetics, ErbB Receptors metabolism, Female, Genes, Reporter, Genetic Vectors chemistry, Genetic Vectors metabolism, Humans, Lentivirus genetics, Lentivirus metabolism, Luciferases genetics, Luciferases metabolism, Lung Neoplasms diagnosis, Lung Neoplasms metabolism, Lung Neoplasms mortality, Male, Mice, Nude, MicroRNAs agonists, MicroRNAs metabolism, Middle Aged, Neoplasm Recurrence, Local diagnosis, Neoplasm Recurrence, Local metabolism, Neoplasm Recurrence, Local mortality, Oligoribonucleotides genetics, Oligoribonucleotides metabolism, Prognosis, Protein Tyrosine Phosphatase, Non-Receptor Type 13 metabolism, Signal Transduction, Survival Analysis, Xenograft Model Antitumor Assays, Adenocarcinoma genetics, Gene Expression Regulation, Neoplastic, Lung Neoplasms genetics, MicroRNAs genetics, Neoplasm Recurrence, Local genetics, Protein Tyrosine Phosphatase, Non-Receptor Type 13 genetics
- Abstract
Aberrant microRNA expression is involved in the regulation of various cellular processes, such as proliferation and metastasis in multiple diseases including cancers. MicroRNA-30e-5p (miR-30e) was previously reported as an oncogenic or tumour suppressing miRNA in some malignancies, but its function in lung adenocarcinoma (LAC) remains largely undefined. In this study, we found that the expression of miR-30e was increased in LAC tissues and cell lines, associated with tumour size and represented an independent prognostic factor for overall survival and recurrence of LAC patients. Further functional experiments showed that knockdown of miR-30e suppressed cell growth while its overexpression promoted growth of LAC cells and xenografts in vitro and in vivo. Mechanistically, PTPN13 was identified as the direct target of miR-30e in LAC, in which PTPN13 expression was down-regulated in LAC tissues and showed the inverse correlation with miR-30e expression. Overexpression of PTPN13 inhibited cell growth and rescued the proliferation-promoting effect of miR-30e through inhibition of the EGFR signalling. Altogether, our findings suggest that miR-30e could function as an oncogene in LAC via targeting PTPN13 and act as a potential therapeutic target for treating LAC., (© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
- Published
- 2017
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