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Your search keyword '"Francolini, Maura"' showing total 8 results
Start Over Author "Francolini, Maura" Journal journal of cell science
8 results on '"Francolini, Maura"'

2. Specific proteolytic cleavage of agrin regulates maturation of the neuromuscular junction

Author

Bolliger, Marc F., primary, Zurlinden, Andreas, additional, Lüscher, Daniel, additional, Bütikofer, Lukas, additional, Shakhova, Olga, additional, Francolini, Maura, additional, Kozlov, Serguei V., additional, Cinelli, Paolo, additional, Stephan, Alexander, additional, Kistler, Andreas D., additional, Rülicke, Thomas, additional, Pelczar, Pawel, additional, Ledermann, Birgit, additional, Fumagalli, Guido, additional, Gloor, Sergio M., additional, Kunz, Beat, additional, and Sonderegger, Peter, additional

Published
2010
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3. Cholesterol reduction impairs exocytosis of synaptic vesicles

Author

Linetti, Anna, primary, Fratangeli, Alessandra, additional, Taverna, Elena, additional, Valnegri, Pamela, additional, Francolini, Maura, additional, Cappello, Valentina, additional, Matteoli, Michela, additional, Passafaro, Maria, additional, and Rosa, Patrizia, additional

Published
2010
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8. Neuronal calcium sensor 1 and phosphatidylinositol 4-OH kinase beta interact in neuronal cells and are translocated to membranes during nucleotide-evoked exocytosis.

Author

Taverna E, Francolini M, Jeromin A, Hilfiker S, Roder J, and Rosa P

Subjects
Animals, Calcium-Binding Proteins ultrastructure, Cell Compartmentation, Cell Membrane metabolism, Cell Membrane ultrastructure, Cell Nucleus metabolism, Cell Nucleus ultrastructure, Cytoplasm metabolism, Cytoplasm ultrastructure, Endoplasmic Reticulum metabolism, Endoplasmic Reticulum ultrastructure, Female, Golgi Apparatus metabolism, Golgi Apparatus ultrastructure, Hippocampus cytology, Hippocampus embryology, Neuronal Calcium-Sensor Proteins, Neurons ultrastructure, PC12 Cells, Protein Binding, Rats, Rats, Sprague-Dawley, Uridine Triphosphate metabolism, 1-Phosphatidylinositol 4-Kinase metabolism, Calcium Signaling, Calcium-Binding Proteins metabolism, Calcium-Binding Proteins physiology, Exocytosis, Neurons metabolism, Neuropeptides metabolism, Protein Transport
Abstract

Neuronal calcium sensor 1 (NCS-1) belongs to a family of EF-hand calcium-binding proteins and is mainly expressed in neurons and neuroendocrine cells, where it causes facilitation of neurotransmitter release through unknown mechanisms. The yeast homologue of NCS-1 has been demonstrated to interact with and regulate the activity of yeast phosphatidylinositol 4-OH kinase beta (PI4Kbeta). However, in neurons and neurosecretory cells NCS-1 has not unequivocally been shown to interact with PI4Kbeta. Here we have compared the subcellular distribution of NCS-1 and PI4Kbeta and investigated whether they are capable of forming complexes. In neurons, both proteins are widely distributed and are present in perikarya and, to a lesser extent, in nerve terminals. A consistent portion of NCS-1 and PIK4beta is cytosolic, whereas a portion of both proteins appears to be associated with the membranes of the endoplasmic reticulum and the Golgi complex. Very small amounts of NCS-1 and PI4Kbeta are present in synaptic vesicles. Our results further demonstrate that in neurosecretory cells, endogenous NCS-1 and PIK4beta interact to form a complex that can be immunoisolated from membrane as well as from cytosolic fractions. Moreover, both proteins can be recruited to membranes when cells are treated with nucleotide receptor agonists known to increase polyphosphoinositide turnover and concomitantly induce exocytosis of secretory vesicles. Finally, in PC12 cells overexpressing NCS-1, the amount of PI4Kbeta associated with the membranes is increased concomitantly with the increased levels of NCS-1 detected in the same membrane fractions. Together, these findings demonstrate that mammalian NCS-1 and PI4Kbeta interact under physiological conditions, which suggest a possible role for NCS-1 in the translocation of PI4Kbeta to target membranes.

Published
2002
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