1. Fermentation scale up for α-arbutin production by Xanthomonas BT-112.
- Author
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Wei, Meng, Ren, Yi, Liu, Changxia, Liu, Ruican, Zhang, Peng, Wei, Yi, Xu, Tao, Wang, Fang, Tan, Tianwei, and Liu, Chunqiao
- Subjects
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ARBUTIN , *FERMENTATION , *XANTHOMONAS , *ENZYMES , *SURFACE active agents , *HYDROQUINONE - Abstract
α-Arbutin is a glycosylated hydroquinone that has an inhibitory function against tyrosinase. The aim of the present study is to develop an efficient and inexpensive method for large-scale production of α-arbutin by using Xanthomonas BT-112 as biocatalyst. To accomplish this goal, various surfactants were tested to enhance the α-arbutin production, and the optimal operational conditions for 30 L jar fermenter were scaled up for a production level of 3000 L with using a constant volumetric oxygen transfer coefficient ( K L a ) and the volumetric aeration rate per volume unit ( Q / V ) as scale-up criteria. Under the optimized conditions, the α-arbutin produced in the presence of 0.4% (w/v) Tween-80 was 124.8% higher than that of the control, and the yield of α-arbutin in 3000 L fermenter was 38.2 g/L with a molar conversion ratio of 93.7% based on the amount of hydroquinone supplied. This result is comparable to the results from laboratory-scale fermenter. Hence, 100-fold scale-up was successfully achieved. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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