1. Enhancement of astaxanthin production from Haematococcus pluvialis mutants by three-stage mutagenesis breeding
- Author
-
Zhaoyan Geng, Ni Wang, Han Sun, Qing Kong, Liangfei Duan, and Bin Guan
- Subjects
0106 biological sciences ,0301 basic medicine ,Mutant ,Mutagenesis (molecular biology technique) ,Bioengineering ,Xanthophylls ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,chemistry.chemical_compound ,Dry weight ,Chlorophyta ,Astaxanthin ,010608 biotechnology ,Biomass ,Haematococcus pluvialis ,biology ,Diphenylamine ,Wild type ,General Medicine ,biology.organism_classification ,Transformation (genetics) ,030104 developmental biology ,Metabolic Engineering ,Biochemistry ,chemistry ,Mutagenesis ,Biotechnology - Abstract
Haematococcus pluvialis was modified for higher astaxanthin production compatible with the superiorities of high biomass and high activity by three-stage mutagenesis breeding. UV irradiation mutants named UV11-4 made an increase on cell dry weight, but showed a longer growth circle than the wild type. On the basis of UV mutants, ethyl methane sulphonate (EMS) mutants E2-5 cut down the latent phase, brought forward and extended the logarithmic phase. The inhibitor diphenylamine (DPA) was employed to screen high-yield astaxanthin producer by the color change of colonies from green to red on solid medium. Via the contravariant cultivation, proliferation and transformation, the mutant DPA12-2 possessed an 1.7-fold astaxanthin production compared to the wild type, reaching 47.21±3.30mg/g dry cells.
- Published
- 2016
- Full Text
- View/download PDF