1. Microbial production of dihomo-γ-linolenic acid by Δ5-desaturase gene-disruptants of Mortierella alpina 1S-4.
- Author
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Kikukawa, Hiroshi, Sakuradani, Eiji, Ando, Akinori, Okuda, Tomoyo, Shimizu, Sakayu, and Ogawa, Jun
- Subjects
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LINOLENIC acids , *MORTIERELLA , *DESATURASES , *DNA ligases , *BIOCONVERSION , *GENE targeting - Abstract
We constructed dihomo-γ-linolenic acid (DGLA)-producing strains with disruption of the Δ5-desaturase ( Δ5ds ) gene, which encodes a key enzyme catalyzing the bioconversion of DGLA to arachidonic acid (ARA), by efficient gene-targeting, using Δlig4 strain of Mortierella alpina 1S-4 as the host. In previous study, we had already identified and disrupted the lig4 gene encoding DNA ligase 4, which involves in non-homologous end joining, in M. alpina 1S-4, and the Δlig4 strain had showed efficient gene-targeting. In this study, the uracil auxotroph of Δlig4 strain was constructed, and then transformed for disruption of Δ5ds . The isolation of nine Δ5ds -disruptants out of 18 isolates indicated that the disruption efficiency was 50%. The ratio of DGLA among the total fatty acids of the Δ5ds -disruptants reached 40.1%; however, no ARA was detected. To our knowledge, this is the first study to report the construction of DGLA-producing transformants by using the efficient gene-targeting system in M. alpina 1S-4. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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