1. Biochemical Characterization of Bovine Brain Myristoyl-CoA:Protein N-Myristoyltransferase Type 2
- Author
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Ashakumary Lakshmikuttyamma, Ponniah Selvakumar, and Rajendra K. Sharma
- Subjects
Article Subject ,Health, Toxicology and Mutagenesis ,lcsh:Biotechnology ,Molecular Sequence Data ,lcsh:Medicine ,Biology ,medicine.disease_cause ,Substrate Specificity ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Genetics ,medicine ,Escherichia coli ,Animals ,Humans ,Magnesium ,Amino Acid Sequence ,Molecular Biology ,Gene ,Peptide sequence ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Fungal protein ,Manganese ,030302 biochemistry & molecular biology ,lcsh:R ,NMT2 ,Brain ,Sodium Dodecyl Sulfate ,General Medicine ,Recombinant Proteins ,3. Good health ,Open reading frame ,Kinetics ,Zinc ,Biochemistry ,Glycine ,Saturated fatty acid ,Solvents ,Molecular Medicine ,Calcium ,Cattle ,Sequence Alignment ,Acyltransferases ,Biotechnology ,Research Article - Abstract
Protein N-myristoylation is a lipidic modification which refers to the covalent attachment of myristate, a 14-carbon saturated fatty acid, to the N-terminal glycine residue of a number of mammalian, viral, and fungal proteins. In this paper, we have cloned the gene coding for myristoyl-CoA:protein N-myristoyltransferase (NMT) fromBos tarusbrain. The open reading frame codes for a 410-amino-acid protein and overexpressed inEscherichia coli. Kinetic studies suggested that bovine brain NMT2 and human NMT1 show significant differences in their peptide substrate specificities. The metal ionCa2+had stimulatory effects on NMT2 activity whileMn2+andZn2+inhibited the enzyme activity. In addition, NMT2 activity was inhibited by various organic solvents and other detergents while NMT1 had a stimulatory effect. Biochemical characterization suggested that both forms of NMT have unique characteristics. Further analysis towards functional role NMT2 will lead the development of therapeutic target for the progression of various diseases such as cancer, cardiovascular diseases, and neurodegenerative diseases.
- Published
- 2009