1. Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole
- Author
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Corinne Mercier, Sirenia González, Mónica Mondragón, Saé Muñiz-Hernández, M. González Del Carmen, M F Cesbron, S L Mondragón-González, Ricardo Mondragón, Departamento de Bioquimica (CINVESTAV-IPN), Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Subdireccion de Investigacion Basica (Instituto Nacional de Cancerologia), Institutio National de Cancerologia, Universidad Veracruzana, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF), Unidad Profesional Interdisciplinaria en Ingenieria y Tecnologias Avanzadas, IPN, and Unidad de Microscopia Electronica (CINVESTAV-IPN)
- Subjects
Health, Toxicology and Mutagenesis ,lcsh:Medicine ,Vacuole ,Residual body ,MESH: Dogs ,Cell membrane ,Mice ,Atypical Residual Bodies ,0302 clinical medicine ,MESH: Animals ,Mice, Inbred BALB C ,0303 health sciences ,MESH: Toxoplasma ,General Medicine ,Cell biology ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,medicine.anatomical_structure ,MESH: Toxoplasmosis ,Molecular Medicine ,Toxoplasma ,Toxoplasmosis ,Research Article ,Biotechnology ,Article Subject ,lcsh:Biotechnology ,030231 tropical medicine ,MESH: Mice, Inbred BALB C ,Biology ,MESH: Vacuoles ,Cell Line ,03 medical and health sciences ,Dogs ,MESH: Cell Proliferation ,lcsh:TP248.13-248.65 ,Genetics ,medicine ,Animals ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Secretion ,MESH: Life Cycle Stages ,MESH: Mice ,Molecular Biology ,Cell Proliferation ,030304 developmental biology ,Life Cycle Stages ,Rosette (schizont appearance) ,Cell Membrane ,lcsh:R ,Toxoplasma gondii ,biology.organism_classification ,medicine.disease ,MESH: Cell Line ,Vacuoles ,MESH: Cell Membrane - Abstract
Toxoplasma gondiiproliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization.
- Published
- 2011
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