1. In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation
- Author
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Kazuyoshi Itoh, Kiichi Fukui, Tsunehito Higashi, Wataru Watanabe, and Sachihiro Matsunaga
- Subjects
Recombinant Fusion Proteins ,Green Fluorescent Proteins ,Biomedical Engineering ,Nanotechnology ,ablation ,Green fluorescent protein ,law.invention ,Biomaterials ,Histones ,Micromanipulation ,In vivo ,law ,femtosecond laser ,Organelle ,Tobacco ,Multiphoton excitation ,Humans ,two-photon microscopy ,Organelles ,Staining and Labeling ,Chemistry ,photoconvertible fluorescent protein ,Fluorescence recovery after photobleaching ,Laser ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Microscopy, Fluorescence, Multiphoton ,Femtosecond ,Biophysics ,nanosurgery ,Intracellular ,Fluorescence Recovery After Photobleaching ,HeLa Cells - Abstract
Wataru Watanabe, Sachhiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, and Kazuyoshi Itoh "In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation," Journal of Biomedical Optics 13(3), 031213 (1 May 2008). https://doi.org/10.1117/1.2939401, Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein. © 2008 Society of Photo-Optical Instrumentation Engineers.
- Published
- 2008