Search

Your search keyword '"Mei Chee Tan"' showing total 4 results
Start Over Author "Mei Chee Tan" Journal journal of biomedical optics
4 results on '"Mei Chee Tan"'

1. Multiscale optical imaging of rare-earth-doped nanocomposites in a small animal model

Author

Mark C. Pierce, Xinyu Zhao, Laura M. Higgins, Vidya Ganapathy, Prabhas V. Moghe, Richard E. Riman, Charles M. Roth, Yang Sheng, Harini Kantamneni, and Mei Chee Tan

Subjects
Materials science, Infrared Rays, Whole body imaging, Biomedical Engineering, Mice, Nude, 02 engineering and technology, 01 natural sciences, law.invention, Nanocomposites, 010309 optics, Biomaterials, Mice, Optical coherence tomography, In vivo, Confocal microscopy, law, 0103 physical sciences, medicine, Image Processing, Computer-Assisted, Animals, Whole Body Imaging, Lung, Microscopy, Confocal, medicine.diagnostic_test, Optical Imaging, Equipment Design, JBO Letters, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, Photon upconversion, Electronic, Optical and Magnetic Materials, Characterization (materials science), Liver, Female, Metals, Rare Earth, 0210 nano-technology, Preclinical imaging, Ex vivo, Biomedical engineering
Abstract

Rare-earth-doped nanocomposites have appealing optical properties for use as biomedical contrast agents, but few systems exist for imaging these materials. We describe the design and characterization of (i) a preclinical system for whole animal in vivo imaging and (ii) an integrated optical coherence tomography/confocal microscopy system for high-resolution imaging of ex vivo tissues. We demonstrate these systems by administering erbium-doped nanocomposites to a murine model of metastatic breast cancer. Short-wave infrared emissions were detected in vivo and in whole organ imaging ex vivo. Visible upconversion emissions and tissue autofluorescence were imaged in biopsy specimens, alongside optical coherence tomography imaging of tissue microstructure. We anticipate that this work will provide guidance for researchers seeking to image these nanomaterials across a wide range of biological models.

Published
2017

2. Line-scanning confocal microscopy for high-resolution imaging of upconverting rare-earth-based contrast agents

Author

Laura M. Higgins, Vidya Ganapathy, Mark C. Pierce, Yang Sheng, Mei Chee Tan, Margot Zevon, Prabhas V. Moghe, Richard E. Riman, and Charles M. Roth

Subjects
Fluorescence-lifetime imaging microscopy, Microscope, Materials science, Optical sectioning, Confocal, Biomedical Engineering, Contrast Media, Sensitivity and Specificity, law.invention, Biomaterials, Optics, law, Confocal microscopy, Cell Line, Tumor, Microscopy, Humans, business.industry, Scanning confocal electron microscopy, Reproducibility of Results, Equipment Design, JBO Letters, Image Enhancement, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Molecular Imaging, Equipment Failure Analysis, Microscopy, Fluorescence, Nanoparticles, Metals, Rare Earth, business, Preclinical imaging, Biomedical engineering
Abstract

Rare-earth (RE) doped nanocomposites emit visible luminescence when illuminated with continuous wave near-infrared light, making them appealing candidates for use as contrast agents in biomedical imaging. However, the emission lifetime of these materials is much longer than the pixel dwell times used in scanning intravital microscopy. To overcome this limitation, we have developed a line-scanning confocal microscope for high-resolution, optically sectioned imaging of samples labeled with RE-based nanomaterials. Instrument performance is quantified using calibrated test objects. NaYF4 : Er,Yb nanocomposites are imaged in vitro, and in ex vivo tissue specimens, with direct comparison to point-scanning confocal microscopy. We demonstrate that the extended pixel dwell time of line-scanning confocal microscopy enables subcellular-level imaging of these nanomaterials while maintaining optical sectioning. The line-scanning approach thus enables microscopic imaging of this emerging class of contrast agents for preclinical studies, with the potential to be adapted for real-time in vivo imaging in the clinic.

Published
2015

3. Multiscale optical imaging of rare-earth-doped nanocomposites in a small animal model.

Author

Higgins, Laura M., Ganapathy, Vidya, Kantamneni, Harini, Xinyu Zhao, Yang Sheng, Mei-Chee Tan, Roth, Charles M., Riman, Richard E., Moghe, Prabhas V., and Pierce, Mark C.

Subjects
RARE earth metals, OPTICAL imaging sensors, IMAGE sensors, NANOCOMPOSITE materials, OPTICAL coherence tomography
Abstract

Rare-earth-doped nanocomposites have appealing optical properties for use as biomedical contrast agents, but few systems exist for imaging these materials. We describe the design and characterization of (i) a preclinical system for whole animal in vivo imaging and (ii) an integrated optical coherence tomography/confocal microscopy system for high-resolution imaging of ex vivo tissues. We demonstrate these systems by administering erbiumdoped nanocomposites to a murine model of metastatic breast cancer. Short-wave infrared emissions were detected in vivo and in whole organ imaging ex vivo. Visible upconversion emissions and tissue autofluorescence were imaged in biopsy specimens, alongside optical coherence tomography imaging of tissue microstructure. We anticipate that this work will provide guidance for researchers seeking to image these nanomaterials across a wide range of biological models. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

4. Line-scanning confocal microscopy for high-resolution imaging of upconverting rare-earth-based contrast agents.

Author

Higgins, Laura M., Zevon, Margot, Ganapathy, Vidya, Yang Sheng, Mei Chee Tan, Riman, Richard E., Roth, Charles M., Moghe, Prabhas V., and Pierce, Mark C.

Subjects
CONFOCAL microscopy, HIGH resolution imaging, RARE earth metal compounds, CONTRAST media, NANOCOMPOSITE materials, PIXELS
Abstract

Rare-earth (RE) doped nanocomposites emit visible luminescence when illuminated with continuous wave near-infrared light, making them appealing candidates for use as contrast agents in biomedical imaging. However, the emission lifetime of these materials is much longer than the pixel dwell times used in scanning intravital microscopy. To overcome this limitation, we have developed a line-scanning confocal microscope for high-resolution, optically sectioned imaging of samples labeled with RE-based nanomaterials. Instrument performance is quantified using calibrated test objects. NaYF4:Er; Yb nanocomposites are imaged in vitro, and in ex vivo tissue specimens, with direct comparison to point-scanning confocal microscopy. We demonstrate that the extended pixel dwell time of line-scanning confocal microscopy enables subcellular-level imaging of these nanomaterials while maintaining optical sectioning. The line-scanning approach thus enablesmicroscopic imaging of this emerging class of contrast agents for preclinical studies, with the potential to be adapted for realtime in vivo imaging in the clinic. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results