1. Monitoring calcium-induced epidermal differentiation in vitro using multiphoton microscopy
- Author
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Marica B. Ericson, Julie Grantham, and Monika Malak
- Subjects
Paper ,keratinocytes ,Biomedical Engineering ,chemistry.chemical_element ,in vitro modeling ,Calcium ,autofluorescence ,01 natural sciences ,010309 optics ,Biomaterials ,Special Section on Selected Topics in Biophotonics: Fluorescence Lifetime Imaging and Optical Micromechanics ,Tissue culture ,Tissue engineering ,Live cell imaging ,0103 physical sciences ,Skin ,Histology ,Cell Differentiation ,live imaging ,Atomic and Molecular Physics, and Optics ,In vitro ,Electronic, Optical and Magnetic Materials ,Cell biology ,Autofluorescence ,Multiphoton fluorescence microscope ,Microscopy, Fluorescence, Multiphoton ,chemistry ,multiphoton microscopy ,Epidermis ,epidermal differentiation - Abstract
Significance: Research in tissue engineering and in vitro organ formation has recently intensified. To assess tissue morphology, the method of choice today is restricted primarily to histology. Thus novel tools are required to enable noninvasive, and preferably label-free, three-dimensional imaging that is more compatible with futuristic organ-on-a-chip models. Aim: We investigate the potential for using multiphoton microscopy (MPM) as a label-free in vitro approach to monitor calcium-induced epidermal differentiation. Approach: In vitro epidermis was cultured at the air–liquid interface in varying calcium concentrations. Morphology and tissue architecture were investigated using MPM based on visualizing cellular autofluorescence. Results: Distinct morphologies corresponding to epidermal differentiation were observed. In addition, Ca2+-induced effects could be distinguished based on the architectural differences in stratification in the tissue cultures. Conclusions: Our study shows that MPM based on cellular autofluorescence enables visualization of Ca2+-induced differentiation in epidermal skin models in vitro. The technique has potential to be further adapted as a noninvasive, label-free, and real-time tool to monitor tissue regeneration and organ formation in vitro.
- Published
- 2020