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According to studies, Hydrogen peroxide (H2O2) is a significant biomarker of physiological processes. Unnormal H2O2 levels in human body may result in diseases. Hence, there is an increasing demand for monitoring the H2O2 concentrations in biological specimen. Here, we construct a non-enzymatic H2O2 electrochemical biosensor based on persimmon tannin-reduced graphene oxide-platinum-palladium nanocomposite (PrG-Pt@Pd NPs) modified with screen-printed carbon electrode (SPE). Combined with suitable electrocatalytic mode for Pt@Pd NPs, high specific large specific volume and good electrical conductivity of RGO, well as the superior sorption capacity of PT for metal-based nano-ion, the PrGPt@Pd striped pleasing heterogeneous catalytic activity toward H2O2 reduction via the synergistic effect. In experimental conditions of optimal, this non-enzymatic electrochemical sensor exhibited excellent electrocatalytic performance for H2O2 with less negative potential (−0.5 V), fast response time (μM, in addition to this LOD of this sensor was 0.059 μM as well as the excellent sensitivity of the sensor (13.696 μA·μM−1·cm−2). Due to excellent specificity, lower detection limit, and good recovery (98.70–99.96%) in the spiked measurements of human serum samples, this non-enzymatic electrochemical biosensor paves the way for H2O2 detection at ultra-low concentrations in physiology and diagnosis.

A persimmon tannin-Aloe vera composite powder (PT-A) was investigated for its capacity to protect against ionizing radiation. Human hepatic cells (L02 cells) and human hepatoma cells (HepG2 cells) were pretreated with different concentrations of PT-A or the single compounds (PT or Aloe vera) and radiated with X-rays. After radiation and post-incubation for 12 h or 24 h, the cell viability, apoptosis, and reactive oxygen species (ROS) production were analyzed by Cell Counting Kit 8 (CCK-8), 2′,7′-dichlorfluorescein diacetate (DCFH-DA) staining, and Hoechst 33258 staining/flow cytometry, respectively. CCK-8 results illustrated that the optimal radiation dose L02 cells was 8 Gy for L02 cells, and the cell activity was 71.72% (IC50 = 412.1 μg/mL) after post-radiation incubation of 12 h. For HepG2 cells, the optimal radiation dose was 8 Gy, and the cell activity was 62.37% (IC50 = 213.0 μg/mL). The cell apoptotic rate was the lowest at a PT-A concentration of 200 μg/mL in L02 cells (4.32%, P < 0.05), and at 100 μg/mL in HepG2 cells (9.80%, P < 0.05). ROS production induced by radiation could be effectively inhibited by 200 μg/mL of PT-A in L02 cells, and by 100 μg/mL of PT-A in HepG2 cells. The PT-A composite has good radioprotective effects on cell vitality and apoptosis of X-rays radiation exposure towards L02 cells and HepG2 cells compared to the persimmon tannin or Aloe vera. Therefore, PT-A composite might be useful as a natural, harmless anti-ionizing radiation agent, and has various clinical application prospects in future.

An electrochemical aptasensor for quantitatively detecting glypican-3 (GPC3) was constructed by combining hemin-reduced graphene oxide-platinum (H-rGO-Pt) nanoparticles (NPs) with reduced graphene oxide-gold (rGO-Au) nanoparticles (NPs). Herein, the rGO-Au NPs deposited onto screen-printed electrodes resulted in signal amplification due to their large surface areas. Meanwhile, highly conductive H-rGO-Pt NPs acted as a sensing medium that improved electrical conductivity and as an indicator for monitoring peak current for determination. A GPC3 aptamer (GPC3apt) with a low equilibrium dissociation constant was used as a bio-recognition molecule. GPC3apt specifically captured GPC3 proteins and formed aptamer-GPC3 complexes, which impeded electron transfer and thus hampered the redox signal of hemin in H-rGO-Pt NPs. This developed electrochemical aptasensor showed a linear response to GPC3 (from 0.001 μg/mL to 10 μg/mL) and had a detection limit of 0.001 μg/mL. This work provides a low-cost and highly sensitive detection with and good recovery for GPC3 and holds great promise for the clinical diagnosis of hepatocellular carcinoma.