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Your search keyword '"Zhang, Jinghai"' showing total 7 results
Start Over Author "Zhang, Jinghai" Journal journal of biological chemistry
7 results on '"Zhang, Jinghai"'

2. A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNav1.4 and hNav1.5 channels while retaining analgesic activity

Author

Xu, Yijia, primary, Meng, Xiangxue, additional, Hou, Xue, additional, Sun, Jianfang, additional, Kong, Xiaohua, additional, Sun, Yuqi, additional, Liu, Zeyu, additional, Ma, Yuanyuan, additional, Niu, Ye, additional, Song, Yongbo, additional, Cui, Yong, additional, Zhao, Mingyi, additional, and Zhang, Jinghai, additional

Published
2017
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3. 93-kDa Twin-domain Serine Protease Inhibitor (Serpin) Has a Regulatory Function on the Beetle Toll Proteolytic Signaling Cascade

Author

Jiang, Rui, primary, Zhang, Bing, additional, Kurokawa, Kenji, additional, So, Young-In, additional, Kim, Eun-Hye, additional, Hwang, Hyun Ok, additional, Lee, Joon-Hee, additional, Shiratsuchi, Akiko, additional, Zhang, Jinghai, additional, Nakanishi, Yoshinobu, additional, Lee, Hee-Seung, additional, and Lee, Bok Luel, additional

Published
2011
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4. Diversity of Innate Immune Recognition Mechanism for Bacterial Polymeric meso-Diaminopimelic Acid-type Peptidoglycan in Insects

Author

Yu, Yang, primary, Park, Ji-Won, additional, Kwon, Hyun-Mi, additional, Hwang, Hyun-Ok, additional, Jang, In-Hwan, additional, Masuda, Akiko, additional, Kurokawa, Kenji, additional, Nakayama, Hiroshi, additional, Lee, Won-Jae, additional, Dohmae, Naoshi, additional, Zhang, Jinghai, additional, and Lee, Bok Luel, additional

Published
2010
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6. Molecular Control of Phenoloxidase-induced Melanin Synthesis in an Insect

Author

Kan, Hongnan, primary, Kim, Chan-Hee, additional, Kwon, Hyun-Mi, additional, Park, Ji-Won, additional, Roh, Kyung-Baeg, additional, Lee, Hanna, additional, Park, Bum-Joon, additional, Zhang, Rong, additional, Zhang, Jinghai, additional, Söderhäll, Kenneth, additional, Ha, Nam-Chul, additional, and Lee, Bok Luel, additional

Published
2008
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7. A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNa v 1.4 and hNa v 1.5 channels while retaining analgesic activity.

Author

Xu Y, Meng X, Hou X, Sun J, Kong X, Sun Y, Liu Z, Ma Y, Niu Y, Song Y, Cui Y, Zhao M, and Zhang J

Subjects
Amino Acid Substitution, Analgesics, Non-Narcotic administration & dosage, Analgesics, Non-Narcotic pharmacology, Analgesics, Non-Narcotic therapeutic use, Animals, Antineoplastic Agents administration & dosage, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Arthropod Proteins genetics, Arthropod Proteins pharmacology, Arthropod Proteins therapeutic use, CHO Cells, Cricetulus, Dose-Response Relationship, Drug, Female, Humans, Male, Mice, NAV1.4 Voltage-Gated Sodium Channel chemistry, NAV1.4 Voltage-Gated Sodium Channel genetics, NAV1.4 Voltage-Gated Sodium Channel metabolism, NAV1.5 Voltage-Gated Sodium Channel chemistry, NAV1.5 Voltage-Gated Sodium Channel genetics, NAV1.5 Voltage-Gated Sodium Channel metabolism, Peptides genetics, Peptides pharmacology, Peptides therapeutic use, Random Allocation, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Scorpion Venoms genetics, Scorpion Venoms pharmacology, Scorpion Venoms therapeutic use, Scorpions, Toxicity Tests, Acute, Toxicity Tests, Subacute, Voltage-Gated Sodium Channel Blockers administration & dosage, Voltage-Gated Sodium Channel Blockers pharmacology, Voltage-Gated Sodium Channel Blockers therapeutic use, Analgesics, Non-Narcotic adverse effects, Antineoplastic Agents adverse effects, Arthropod Proteins adverse effects, Mutation, Peptides adverse effects, Scorpion Venoms adverse effects, Voltage-Gated Sodium Channel Blockers adverse effects
Abstract

Scorpion toxins can kill other animals by inducing paralysis and arrhythmia, which limits the potential applications of these agents in the clinical management of diseases. Antitumor-analgesic peptide (AGAP), purified from Buthus martensii Karsch, has been proved to possess analgesic and antitumor activities. Trp 38 , a conserved aromatic residue of AGAP, might play an important role in mediating AGAP activities according to the sequence and homology-modeling analyses. Therefore, an AGAP mutant, W38G, was generated, and effects of both AGAP and the mutant W38G were examined by whole-cell patch clamp techniques on the sodium channels hNa v 1.4 and hNa v 1.5, which were closely associated with the biotoxicity of skeletal and cardiac muscles, respectively. The data showed that both W38G and AGAP inhibited the peak currents of hNa v 1.4 and hNa v 1.5; however, W38G induced a much weaker inhibition of both channels than AGAP. Accordingly, W38G exhibited much less toxic effect on both skeletal and cardiac muscles than AGAP in vivo The analgesic activity of W38G and AGAP were verified in vivo as well, and W38G retained analgesic activity similar to AGAP. Inhibition to both Na v 1.7 and Na v 1.8 was involved in the analgesic mechanism of AGAP and W38G. These findings indicated that Trp 38 was a key amino acid involved in the biotoxicity of AGAP, and the AGAP mutant W38G might be a safer alternative for clinical application because it retains the analgesic efficacy with less toxicity to skeletal and cardiac muscles., (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)

Published
2017
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