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2. The dominant negative Ras mutant, N17Ras, can inhibit signaling independently of blocking Ras activation.

3. The calcium/calmodulin-dependent protein phosphatase calcineurin is the major Elk-1 phosphatase.

4. Purification of a skeletal muscle polypeptide which stimulates choline acetyltransferase activity in cultured spinal cord neurons.

5. Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation.

6. Fission yeast Duf89 and Duf8901 are cobalt/nickel-dependent phosphatase-pyrophosphatases that act via a covalent aspartyl-phosphate intermediate.

7. Activity and structure of Pseudomonas putida MPE, a manganese-dependent single-strand DNA endonuclease encoded in a nucleic acid repair gene cluster.

8. Structures of ATP-bound DNA ligase D in a closed domain conformation reveal a network of amino acid and metal contacts to the ATP phosphates.

9. Simulations of the regulatory ACT domain of human phenylalanine hydroxylase (PAH) unveil its mechanism of phenylalanine binding.

10. Characterization of Lhr-Core DNA helicase and manganese- dependent DNA nuclease components of a bacterial gene cluster encoding nucleic acid repair enzymes.

11. A long noncoding (lnc)RNA governs expression of the phosphate transporter Pho84 in fission yeast and has cascading effects on the flanking prt lncRNA and pho1 genes.

12. Phenotypic dissection of the mouse Ren1d knockout by complementation with human renin.

13. Mycobacterium smegmatis Lhr Is a DNA-dependent ATPase and a 3'-to-5' DNA translocase and helicase that prefers to unwind 3'-tailed RNA:DNA hybrids.

14. HIV entry and envelope glycoprotein-mediated fusion.

15. Kinetic analysis of DNA strand joining by Chlorella virus DNA ligase and the role of nucleotidyltransferase motif VI in ligase adenylylation.

16. Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions.

17. RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.

18. Structure-function analysis of the OB and latch domains of chlorella virus DNA ligase.

19. Functional dissection of the DNA interface of the nucleotidyltransferase domain of chlorella virus DNA ligase.

20. RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.

21. RNA 3'-phosphate cyclase (RtcA) catalyzes ligase-like adenylylation of DNA and RNA 5'-monophosphate ends.

22. Double strand break unwinding and resection by the mycobacterial helicase-nuclease AdnAB in the presence of single strand DNA-binding protein (SSB).

23. An exceptionally potent inducer of cytoprotective enzymes: elucidation of the structural features that determine inducer potency and reactivity with Keap1.

24. The C5a receptor (C5aR) C5L2 is a modulator of C5aR-mediated signal transduction.

25. Gap filling activities of Pseudomonas DNA ligase D (LigD) polymerase and functional interactions of LigD with the DNA end-binding Ku protein.

26. Characterization of the mycobacterial AdnAB DNA motor provides insights into the evolution of bacterial motor-nuclease machines.

27. Calcium is essential for the major pseudopilin in the type 2 secretion system.

28. DNA ligases: progress and prospects.

29. Angiotensin-converting enzyme is a modifier of hypertensive end organ damage.

30. Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA).

31. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways.

32. A phosphate-binding histidine of binuclear metallophosphodiesterase enzymes is a determinant of 2',3'-cyclic nucleotide phosphodiesterase activity.

33. Polyphosphatase activity of CthTTM, a bacterial triphosphate tunnel metalloenzyme.

34. Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.

35. Chemical and traditional mutagenesis of vaccinia DNA topoisomerase provides insights to cleavage site recognition and transesterification chemistry.

36. Bacterial nonhomologous end joining ligases preferentially seal breaks with a 3'-OH monoribonucleotide.

37. Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair.

38. Novel triphosphate phosphohydrolase activity of Clostridium thermocellum TTM, a member of the triphosphate tunnel metalloenzyme superfamily.

39. Mutational analysis of Encephalitozoon cuniculi mRNA cap (guanine-N7) methyltransferase, structure of the enzyme bound to sinefungin, and evidence that cap methyltransferase is the target of sinefungin's antifungal activity.

40. Nonpolar nucleobase analogs illuminate requirements for site-specific DNA cleavage by vaccinia topoisomerase.

41. Nucleotide misincorporation, 3'-mismatch extension, and responses to abasic sites and DNA adducts by the polymerase component of bacterial DNA ligase D.

42. Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase.

43. Poxvirus mRNA cap methyltransferase. Bypass of the requirement for the stimulatory subunit by mutations in the catalytic subunit and evidence for intersubunit allostery.

44. The estrogen-responsive B box protein is a novel regulator of the retinoid signal.

45. Substrate specificity and structure-function analysis of the 3'-phosphoesterase component of the bacterial NHEJ protein, DNA ligase D.

46. Crystal structure and nonhomologous end-joining function of the ligase component of Mycobacterium DNA ligase D.

47. Crystal structure of a bacterial type IB DNA topoisomerase reveals a preassembled active site in the absence of DNA.

48. An anti-inflammatory function for the complement anaphylatoxin C5a-binding protein, C5L2.

49. Different strategies for carboxyl-terminal domain (CTD) recognition by serine 5-specific CTD phosphatases.

50. Essential constituents of the 3'-phosphoesterase domain of bacterial DNA ligase D, a nonhomologous end-joining enzyme.

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