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Your search keyword '"Gerasimenko, Oleg V."' showing total 12 results
Start Over Author "Gerasimenko, Oleg V." Journal journal of biological chemistry
12 results on '"Gerasimenko, Oleg V."'

12. Localization of Ca2+Extrusion Sites in Pancreatic Acinar Cells (∗)

Author

Belan, Pavel V., Gerasimenko, Oleg V., Tepikin, Alexei V., and Petersen, Ole H.

Abstract

We have investigated the localization of Ca2+extrusion sites in mouse pancreatic acinar cells. Employing a new technique, in which high resolution localization of cellular Ca2+exit is achieved by confocal microscopy and a Ca2+-sensitive fluorescent probe coupled to heavy dextran to slow down diffusion of extracellular Ca2+, it is shown directly that the secretory pole (secretory granule area) is the major site for Ca2+extrusion following agonist stimulation. This Ca2+extrusion appears not to be a consequence of exocytosis, as assessment of secretion under our experimental conditions (low external Ca2+concentration, room temperature) using the technique of monitoring quinacrine fluorescence shows little loss of secretory granules in spite of sustained Ca2+exit. We conclude that Ca2+is primarily extruded by Ca2+pumps from the secretory pole and propose that this process is useful for maintaining a high Ca2+concentration in the acinar lumen, which is necessary for promotion of endocytosis.

Published
1996
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