244 results on '"Amy, L."'
Search Results
2. Differential Dorso-ventral Distributions of Kv4.2 and HCN Proteins Confer Distinct Integrative Properties to Hippocampal CA1 Pyramidal Cell Distal Dendrites*
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Marcelin, Béatrice, Lugo, Joaquin N, Brewster, Amy L, Liu, Zhiqiang, Lewis, Alan S, McClelland, Shawn, Chetkovich, Dane M, Baram, Tallie Z, Anderson, Anne E, Becker, Albert, Esclapez, Monique, and Bernard, Christophe
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Neurosciences ,Underpinning research ,1.1 Normal biological development and functioning ,Neurological ,Animals ,Cyclic Nucleotide-Gated Cation Channels ,Dendrites ,Down-Regulation ,Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ,Ion Channels ,Nerve Tissue Proteins ,Organ Specificity ,Potassium Channels ,Pyramidal Cells ,Rats ,Shal Potassium Channels ,Transcription ,Genetic ,Up-Regulation ,Chemical Sciences ,Biological Sciences ,Medical and Health Sciences ,Biochemistry & Molecular Biology - Abstract
The dorsal and ventral regions of the hippocampus perform different functions. Whether the integrative properties of hippocampal cells reflect this heterogeneity is unknown. We focused on dendrites where most synaptic input integration takes place. We report enhanced backpropagation and theta resonance and decreased summation of synaptic inputs in ventral versus dorsal CA1 pyramidal cell distal dendrites. Transcriptional Kv4.2 down-regulation and post-transcriptional hyperpolarization-activated cyclic AMP-gated channel (HCN1/2) up-regulation may underlie these differences, respectively. Our results reveal differential dendritic integrative properties along the dorso-ventral axis, reflecting diverse computational needs.
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- 2012
3. The GTPase-activating protein p120RasGAP has an evolutionarily conserved 'FLVR-unique' SH2 domain
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Titus J. Boggon, Amy L. Stiegler, Rachel Jaber Chehayeb, and Jessica Wang
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0301 basic medicine ,030102 biochemistry & molecular biology ,GTPase-activating protein ,Chemistry ,Phosphopeptide ,Stereochemistry ,p120 GTPase Activating Protein ,Isothermal titration calorimetry ,Cell Biology ,Crystallography, X-Ray ,SH2 domain ,Biochemistry ,Homology (biology) ,Protein–protein interaction ,Evolution, Molecular ,src Homology Domains ,03 medical and health sciences ,030104 developmental biology ,Protein structure ,Humans ,Editors' Picks ,Molecular Biology ,Proto-oncogene tyrosine-protein kinase Src - Abstract
The Src homology 2 (SH2) domain has a highly conserved architecture that recognizes linear phosphotyrosine motifs and is present in a wide range of signaling pathways across different evolutionary taxa. A hallmark of SH2 domains is the arginine residue in the conserved FLVR motif that forms a direct salt bridge with bound phosphotyrosine. Here, we solve the X-ray crystal structures of the C-terminal SH2 domain of p120RasGAP (RASA1) in its apo and peptide-bound form. We find that the arginine residue in the FLVR motif does not directly contact pTyr(1087) of a bound phosphopeptide derived from p190RhoGAP; rather, it makes an intramolecular salt bridge to an aspartic acid. Unexpectedly, coordination of phosphotyrosine is achieved by a modified binding pocket that appears early in evolution. Using isothermal titration calorimetry, we find that substitution of the FLVR arginine R377A does not cause a significant loss of phosphopeptide binding, but rather a tandem substitution of R398A (SH2 position βD4) and K400A (SH2 position βD6) is required to disrupt the binding. These results indicate a hitherto unrecognized diversity in SH2 domain interactions with phosphotyrosine and classify the C-terminal SH2 domain of p120RasGAP as “FLVR-unique.”
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- 2020
4. The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier
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Robert F. L. Steuart, Kofi L. P. Stevens, Carl J. Mousley, Paris F. White, Hasindu G. Dassanayake, Aleshanee L. Paxman, Lamprini Baklous, Christopher M. Witham, Benjamin L. Schulz, Colin J. Stirling, and Amy L. Black
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0301 basic medicine ,Sec61 ,Saccharomyces cerevisiae Proteins ,Saccharomyces cerevisiae ,Gating ,Endoplasmic Reticulum ,Biochemistry ,Permeability ,03 medical and health sciences ,Translocase ,Amino Acid Sequence ,Molecular Biology ,Integral membrane protein ,Secretory pathway ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Endoplasmic reticulum ,C-terminus ,Cell Biology ,Endoplasmic Reticulum Stress ,Translocon ,Cell biology ,Protein Transport ,030104 developmental biology ,Multiprotein Complexes ,Protein Biosynthesis ,Mutation ,biology.protein ,SEC Translocation Channels - Abstract
The endoplasmic reticulum (ER) is the entry point to the secretory pathway and major site of protein biogenesis. Translocation of secretory and integral membrane proteins across or into the ER membrane occurs via the evolutionarily conserved Sec61 complex, a heterotrimeric channel that comprises the Sec61p/Sec61α, Sss1p/Sec61γ, and Sbh1p/Sec61β subunits. In addition to forming a protein-conducting channel, the Sec61 complex also functions to maintain the ER permeability barrier, preventing the mass free flow of essential ER-enriched molecules and ions. Loss in Sec61 integrity is detrimental and implicated in the progression of disease. The Sss1p/Sec61γ C terminus is juxtaposed to the key gating module of Sec61p/Sec61α, and we hypothesize it is important for gating the ER translocon. The ER stress response was found to be constitutively induced in two temperature-sensitive sss1 mutants (sss1(ts)) that are still proficient to conduct ER translocation. A screen to identify intergenic mutations that allow for sss1(ts) cells to grow at 37 °C suggests the ER permeability barrier to be compromised in these mutants. We propose the extreme C terminus of Sss1p/Sec61γ is an essential component of the gating module of the ER translocase and is required to maintain the ER permeability barrier.
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- 2020
5. Extracellular cathepsin Z signals through the α5 integrin and augments NLRP3 inflammasome activation
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Campden, Rhiannon I., primary, Warren, Amy L., additional, Greene, Catherine J., additional, Chiriboga, Jose A., additional, Arnold, Corey R., additional, Aggarwal, Devin, additional, McKenna, Neil, additional, Sandall, Christina F., additional, MacDonald, Justin A., additional, and Yates, Robin M., additional
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- 2021
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6. Loss of the tumor suppressor BIN1 enables ATM Ser/Thr kinase activation by the nuclear protein E2F1 and renders cancer cells resistant to cisplatin
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Slovénie Pyndiah, Daitoku Sakamuro, Amy L. Abdulovic-Cui, Erica K. Cassimere, Watson P. Folk, Joanna C. Johnson, Alpana Kumari, and Tetsushi Iwasaki
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0301 basic medicine ,DNA Repair ,Transcription, Genetic ,Tumor suppressor gene ,Cell Cycle Proteins ,Ataxia Telangiectasia Mutated Proteins ,DNA and Chromosomes ,Biochemistry ,Histones ,03 medical and health sciences ,Cell Line, Tumor ,Neoplasms ,medicine ,Humans ,E2F1 ,DNA Breaks, Double-Stranded ,Molecular Biology ,Adaptor Proteins, Signal Transducing ,E2F2 ,Cisplatin ,MRE11 Homologue Protein ,030102 biochemistry & molecular biology ,Chemistry ,Tumor Suppressor Proteins ,Nuclear Proteins ,DNA, Neoplasm ,Cell Biology ,G2-M DNA damage checkpoint ,medicine.disease ,Acid Anhydride Hydrolases ,Cell biology ,MDC1 ,DNA-Binding Proteins ,DNA Repair Enzymes ,030104 developmental biology ,MRN complex ,Drug Resistance, Neoplasm ,Ataxia-telangiectasia ,biological phenomena, cell phenomena, and immunity ,E2F1 Transcription Factor ,Signal Transduction ,medicine.drug - Abstract
The tumor suppressor bridging integrator 1 (BIN1) is a corepressor of the transcription factor E2F1 and inhibits cell-cycle progression. BIN1 also curbs cellular poly(ADP-ribosyl)ation (PARylation) and increases sensitivity of cancer cells to DNA-damaging therapeutic agents such as cisplatin. However, how BIN1 deficiency, a hallmark of advanced cancer cells, increases cisplatin resistance remains elusive. Here, we report that BIN1 inactivates ataxia telangiectasia–mutated (ATM) serine/threonine kinase, particularly when BIN1 binds E2F1. BIN1 + 12A (a cancer-associated BIN1 splicing variant) also inhibited cellular PARylation, but only BIN1 increased cisplatin sensitivity. BIN1 prevented E2F1 from transcriptionally activating the human ATM promoter, whereas BIN1 + 12A did not physically interact with E2F1. Conversely, BIN1 loss significantly increased E2F1-dependent formation of MRE11A/RAD50/NBS1 DNA end-binding protein complex and efficiently promoted ATM autophosphorylation. Even in the absence of dsDNA breaks (DSBs), BIN1 loss promoted ATM-dependent phosphorylation of histone H2A family member X (forming γH2AX, a DSB biomarker) and mediator of DNA damage checkpoint 1 (MDC1, a γH2AX-binding adaptor protein for DSB repair). Of note, even in the presence of transcriptionally active (i.e. proapoptotic) TP53 tumor suppressor, BIN1 loss generally increased cisplatin resistance, which was conversely alleviated by ATM inactivation or E2F1 reduction. However, E2F2 or E2F3 depletion did not recapitulate the cisplatin sensitivity elicited by E2F1 elimination. Our study unveils an E2F1-specific signaling circuit that constitutively activates ATM and provokes cisplatin resistance in BIN1-deficient cancer cells and further reveals that γH2AX emergence may not always reflect DSBs if BIN1 is absent.
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- 2019
7. FoxL2 and Smad3 Coordinately Regulate Follistatin Gene Transcription
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Blount, Amy L., Schmidt, Karsten, Justice, Nicholas J., Vale, Wylie W., Fischer, Wolfgang H., and Bilezikjian, Louise M.
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- 2009
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8. Extracellular cathepsin Z signals through the α5 integrin and augments NLRP3 inflammasome activation
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Justin A. MacDonald, Catherine J. Greene, Christina F. Sandall, Neil McKenna, Rhiannon I. Campden, Jose A. Chiriboga, Robin M. Yates, Devin Aggarwal, Amy L. Warren, and Corey R. Arnold
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Inflammasomes ,Interleukin-1beta ,Integrin alpha5 ,Biochemistry ,Mice ,0302 clinical medicine ,0303 health sciences ,biology ,Chemistry ,interleukin-1 (IL-1) ,Inflammasome ,Silicon Dioxide ,PMA, phorbol myristate acetate ,Cysteine protease ,3. Good health ,Cell biology ,silica ,LPS, lipopolysaccharide ,medicine.symptom ,RGD, arginine-glycine-asparigine ,Research Article ,medicine.drug ,integrin ,Silicosis ,Integrin ,Inflammation ,MSU, monosodium urate ,MS, multiple sclerosis ,EAE, encephalomyelitis ,03 medical and health sciences ,NLRP3 ,FBS, fetal bovine serum ,inflammasome ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Extracellular ,Animals ,APC, antigen-presenting cells ,Molecular Biology ,BMDC, bone marrow-derived dendritic cells ,030304 developmental biology ,Cathepsin ,Cathepsin Z ,Cell Biology ,arginine-glycine-aspartic acid (RGD) domain ,Cell culture ,biology.protein ,030217 neurology & neurosurgery - Abstract
Respiratory silicosis is a preventable occupational disease that develops secondary to the aspiration of crystalline silicon dioxide (silica) into the lungs, activation of the NLRP3 inflammasome, and IL-1β production. Cathepsin Z has been associated with the development of inflammation and IL-1β production; however, the mechanism of how cathepsin Z leads to IL-1β production is unknown. Here, the requirement for cathepsin Z in silicosis was determined using wildtype mice and mice deficient in cathepsin Z. The activation of the NLRP3 inflammasome in macrophages was studied using wildtype and cathepsin Z-deficient bone marrow-derived murine dendritic cells and the human monocytic cell line THP-1. Cells were activated with silica, and IL-1β release was determined using enzyme-linked immunosorbent assay or IL-1β bioassays. The relative contribution of the active domain or integrin-binding domain of cathepsin Z was studied using recombinant cathepsin Z constructs and the α5 integrin neutralizing antibody. We report that the lysosomal cysteine protease cathepsin Z potentiates the development of inflammation associated with respiratory silicosis by augmenting NLRP3 inflammasome-derived IL-1β expression in response to silica. Secreted cathepsin Z functions non-proteolytically via the internal integrin-binding domain to impact caspase-1 activation and the production of active IL-1β through integrin α5 without affecting transcription levels of NLRP3 inflammasome components. This work reveals a regulatory pathway for the NLRP3 inflammasome that occurs in an outside-in fashion and provides a link between extracellular cathepsin Z and inflammation. Furthermore, it reveals a level of NLRP3 inflammasome regulation that has previously only been found downstream of extracellular pathogens.
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- 2022
9. TNF receptor-associated factor 3 restrains B-cell receptor signaling in normal and malignant B cells
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Whillock, Amy L., primary, Ybarra, Tiffany K., additional, and Bishop, Gail A., additional
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- 2021
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10. Extracellular cathepsin Z signals through the α5 integrin and augments NLRP3 inflammasome activation.
- Author
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Campden, Rhiannon I., Warren, Amy L., Greene, Catherine J., Chiriboga, Jose A., Arnold, Corey R., Aggarwal, Devin, McKenna, Neil, Sandall, Christina F., MacDonald, Justin A., and Yates, Robin M.
- Subjects
- *
NLRP3 protein , *INFLAMMASOMES , *ENZYME-linked immunosorbent assay , *OCCUPATIONAL diseases , *DENDRITIC cells , *ELASTASES , *INTEGRINS - Abstract
Respiratory silicosis is a preventable occupational disease that develops secondary to the aspiration of crystalline silicon dioxide (silica) into the lungs, activation of the NLRP3 inflammasome, and IL-1β production. Cathepsin Z has been associated with the development of inflammation and IL-1β production; however, the mechanism of how cathepsin Z leads to IL-1β production is unknown. Here, the requirement for cathepsin Z in silicosis was determined using WT mice and mice deficient in cathepsin Z. The activation of the NLRP3 inflammasome in macrophages was studied using WT and cathepsin Z-deficient bone marrow-derived murine dendritic cells and the human monocytic cell line THP-1. The cells were activated with silica, and IL-1β release was determined using enzyme-linked immunosorbent assay or IL-1β bioassays. The relative contribution of the active domain or integrin-binding domain of cathepsin Z was studied using recombinant cathepsin Z constructs and the α5 integrin neutralizing antibody. We report that the lysosomal cysteine protease cathepsin Z potentiates the development of inflammation associated with respiratory silicosis by augmenting NLRP3 inflammasome-derived IL-1β expression in response to silica. The secreted cathepsin Z functions nonproteolytically via the internal integrin-binding domain to impact caspase-1 activation and the production of active IL-1β through integrin α5 without affecting the transcription levels of NLRP3 inflammasome components. This work reveals a regulatory pathway for the NLRP3 inflammasome that occurs in an outside-in fashion and provides a link between extracellular cathepsin Z and inflammation. Furthermore, it reveals a level of NLRP3 inflammasome regulation that has previously only been found downstream of extracellular pathogens. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
11. The GTPase-activating protein p120RasGAP has an evolutionarily conserved “FLVR-unique” SH2 domain
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Jaber Chehayeb, Rachel, primary, Wang, Jessica, additional, Stiegler, Amy L., additional, and Boggon, Titus J., additional
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- 2020
- Full Text
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12. The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier
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Witham, Christopher M., primary, Dassanayake, Hasindu G., additional, Paxman, Aleshanee L., additional, Stevens, Kofi L.P., additional, Baklous, Lamprini, additional, White, Paris F., additional, Black, Amy L., additional, Steuart, Robert F.L., additional, Stirling, Colin J., additional, Schulz, Benjamin L., additional, and Mousley, Carl J., additional
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- 2020
- Full Text
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13. TNF receptor-associated factor 3 restrains B-cell receptor signaling in normal and malignant B cells
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Gail A. Bishop, Amy L. Whillock, and Tiffany K. Ybarra
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Male ,0301 basic medicine ,T-Lymphocytes ,Syk ,NIK, NF-κB-inducing kinase ,Biochemistry ,WB, western blot ,immunology ,B-cell receptor (BCR) ,Mice ,hemic and lymphatic diseases ,TRAF, TNF receptor-associated factor ,Agammaglobulinaemia Tyrosine Kinase ,Phosphorylation ,BAFF, B-cell activating factor ,Ub, ubiquitin ,Mice, Knockout ,TNF, tumor necrosis factor ,B-Lymphocytes ,biology ,Chemistry ,BCR, B-cell antigen receptor ,breakpoint cluster region ,PLCγ, phospholipase C gamma ,STAT, signal transducer and activator of transcription ,Btk ,TNF Receptor-Associated Factor 3 ,inhibition mechanism ,Lyn, Lck/yes novel tyrosine kinase ,Female ,WCL, whole-cell lysate ,MZ, marginal zone ,DLBCL, diffuse large B-cell lymphoma ,CD79 Antigens ,Research Article ,TLR, Toll-like receptor ,Signal Transduction ,Cell signaling ,LMP1, latent membrane protein 1 ,Syk, spleen-associated tyrosine kinase ,Receptors, Antigen, B-Cell ,lymphoma ,lymphocyte ,Btk, Bruton's tyrosine kinase ,SEM, standard error of the mean ,spleen tyrosine kinase (Syk) ,Erk, extracellular signal-regulated kinase ,03 medical and health sciences ,SHP, Src homology region 2 domain-containing phosphatase ,LYN ,cell signaling ,Animals ,Syk Kinase ,FO, follicular ,Molecular Biology ,TNF receptor-associated factor (TRAF) ,IκBα, NF-κB inhibitor alpha ,CD40 ,030102 biochemistry & molecular biology ,PI3K, phosphatidyl inositol-3 kinase ,T-cell receptor ,ITAM, immunoreceptor tyrosine-based activation motif ,Cell Biology ,TNF Receptor-Associated Factor 2 ,MAPK ,SHIP, SH2 domain-containing inositol polyphosphate 5-phosphatase ,Mice, Inbred C57BL ,030104 developmental biology ,TCR, T-cell receptor ,biology.protein ,Cancer research ,BCM, B-cell medium ,MAPK, mitogen-activated protein kinase ,CD80 - Abstract
TRAF3 has diverse signaling functions, which vary by cell type. Uniquely in B lymphocytes, TRAF3 inhibits homeostatic survival. Highlighting the role of TRAF3 as a tumor suppressor, loss-of-function TRAF3 mutations are associated with human B-cell malignancies, while B-cell-specific deletion of TRAF3 in mice leads to autoimmunity and lymphoma development. The role of TRAF3 in inhibiting noncanonical NF-κB activation, CD40 and BAFF-R signaling to B cells is well documented. In contrast, TRAF3 enhances many T-cell effector functions, through associating with and enhancing signaling by the T-cell receptor (TCR)-CD28 complex. The present study was designed to determine the role of TRAF3 in signaling via the B-cell antigen receptor (BCR). The BCR is crucial for antigen recognition, survival, proliferation, and antibody production, and defects in BCR signaling can promote abnormal survival of malignant B cells. Here, we show that TRAF3 is associated with both CD79B and the BCR-activated kinases Syk and Btk following BCR stimulation. BCR-induced phosphorylation of Syk and additional downstream kinases was increased in TRAF3−/− B cells, with regulation observed in both follicular and marginal zone B-cell subsets. BCR stimulation of TRAF3−/− B cells resulted in increased surface expression of MHC-II, CD80, and CD86 molecules. Interestingly, increased survival of TRAF3−/− primary B cells was resistant to inhibition of Btk, while TRAF3-deficient malignant B-cell lines showed enhanced sensitivity. TRAF3 serves to restrain normal and malignant BCR signaling, with important implications for its role in normal B-cell biology and abnormal survival of malignant B cells.
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- 2021
14. Loss of the tumor suppressor BIN1 enables ATM Ser/Thr kinase activation by the nuclear protein E2F1 and renders cancer cells resistant to cisplatin
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Folk, Watson P., primary, Kumari, Alpana, additional, Iwasaki, Tetsushi, additional, Pyndiah, Slovénie, additional, Johnson, Joanna C., additional, Cassimere, Erica K., additional, Abdulovic-Cui, Amy L., additional, and Sakamuro, Daitoku, additional
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- 2019
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15. Structural Determinants for Binding of Sorting Nexin 17 (SNX17) to the Cytoplasmic Adaptor Protein Krev Interaction Trapped 1 (KRIT1)
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Weizhi Liu, Amy L. Stiegler, Rong Zhang, and Titus J. Boggon
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Models, Molecular ,Endosome ,Sorting Nexins ,Amino Acid Motifs ,Immunoblotting ,Plasma protein binding ,Biology ,Crystallography, X-Ray ,Binding, Competitive ,Biochemistry ,Protein–protein interaction ,Proto-Oncogene Proteins ,Protein Interaction Mapping ,Humans ,Amino Acid Sequence ,Structural motif ,KRIT1 Protein ,Molecular Biology ,Binding Sites ,Sequence Homology, Amino Acid ,FERM domain ,Signal transducing adaptor protein ,Cell Biology ,Protein Structure, Tertiary ,Cell biology ,P-Selectin ,Sorting nexin ,Protein Structure and Folding ,Mutagenesis, Site-Directed ,Microtubule-Associated Proteins ,Protein Binding - Abstract
Sorting nexin 17 (SNX17) is a member of the family of cytoplasmic sorting nexin adaptor proteins that regulate endosomal trafficking of cell surface proteins. SNX17 localizes to early endosomes where it directly binds NPX(Y/F) motifs in the cytoplasmic tails of its target receptors to mediate their rates of endocytic internalization, recycling, and/or degradation. SNX17 has also been implicated in mediating cell signaling and can interact with cytoplasmic proteins. KRIT1 (Krev interaction trapped 1), a cytoplasmic adaptor protein associated with cerebral cavernous malformations, has previously been shown to interact with SNX17. Here, we demonstrate that SNX17 indeed binds directly to KRIT1 and map the binding to the second Asn-Pro-Xaa-Tyr/Phe (NPX(Y/F)) motif in KRIT1. We further characterize the interaction as being mediated by the FERM domain of SNX17. We present the co-crystal structure of SNX17-FERM with the KRIT1-NPXF2 peptide to 3.0 Å resolution and demonstrate that the interaction is highly similar in structure and binding affinity to that between SNX17 and P-selectin. We verify the molecular details of the interaction by site-directed mutagenesis and pulldown assay and thereby confirm that the major binding site for SNX17 is confined to the NPXF2 motif in KRIT1. Taken together, our results verify a direct interaction between SNX17 and KRIT1 and classify KRIT1 as a SNX17 binding partner.
- Published
- 2014
16. Small Substrate Transport and Mechanism of a Molybdate ATP Binding Cassette Transporter in a Lipid Environment
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Amy L. Davidson, Alistair Harrison, Austin J. Rice, Frances Joan D. Alvarez, and Heather W. Pinkett
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Lipid Bilayers ,ATP-binding cassette transporter ,Molybdate ,Biology ,Biochemistry ,Cell membrane ,03 medical and health sciences ,chemistry.chemical_compound ,Adenosine Triphosphate ,Bacterial Proteins ,medicine ,Lipid bilayer ,Molecular Biology ,Ion transporter ,Nutrient Uptake ,030304 developmental biology ,Molybdenum ,0303 health sciences ,Ion Transport ,Reverse Transcriptase Polymerase Chain Reaction ,030306 microbiology ,Membrane transport protein ,Hydrolysis ,Electron Paramagnetic Resonance (EPR) ,Cell Membrane ,Electron Spin Resonance Spectroscopy ,Membrane Transport Proteins ,Biological membrane ,Gene Expression Regulation, Bacterial ,Cell Biology ,Membrane transport ,Lipids ,Haemophilus influenzae ,ABC Transporter ,ATP ,medicine.anatomical_structure ,Membrane Transport ,chemistry ,Liposomes ,Mutation ,Periplasm ,biology.protein ,ATP-Binding Cassette Transporters ,Molecular Biophysics - Abstract
Background: Multiple ABC transporters work in concert to transport the same substrate. Results: MolBC-A allows for additional Mo uptake in periods of high external molybdate concentration. Conclusion: Utilizing the established transport mechanism, molybdate uptake is concentration-dependent. Significance: Our studies address the impact the lipid environment has on the mechanism of MolBC-A as well as the role this transporter plays in molybdate uptake., Embedded in the plasma membrane of all bacteria, ATP binding cassette (ABC) importers facilitate the uptake of several vital nutrients and cofactors. The ABC transporter, MolBC-A, imports molybdate by passing substrate from the binding protein MolA to a membrane-spanning translocation pathway of MolB. To understand the mechanism of transport in the biological membrane as a whole, the effects of the lipid bilayer on transport needed to be addressed. Continuous wave-electron paramagnetic resonance and in vivo molybdate uptake studies were used to test the impact of the lipid environment on the mechanism and function of MolBC-A. Working with the bacterium Haemophilus influenzae, we found that MolBC-A functions as a low affinity molybdate transporter in its native environment. In periods of high extracellular molybdate concentration, H. influenzae makes use of parallel molybdate transport systems (MolBC-A and ModBC-A) to take up a greater amount of molybdate than a strain with ModBC-A alone. In addition, the movement of the translocation pathway in response to nucleotide binding and hydrolysis in a lipid environment is conserved when compared with in-detergent analysis. However, electron paramagnetic resonance spectroscopy indicates that a lipid environment restricts the flexibility of the MolBC translocation pathway. By combining continuous wave-electron paramagnetic resonance spectroscopy and substrate uptake studies, we reveal details of molybdate transport and the logistics of uptake systems that employ multiple transporters for the same substrate, offering insight into the mechanisms of nutrient uptake in bacteria.
- Published
- 2014
17. Estradiol Stimulates Glucose Metabolism via 6-Phosphofructo-2-kinase (PFKFB3)
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Lilibeth Lanceta, Julie O'Neal, Daniel Alan Kerr, Amy L. Clem, Sucheta Telang, Yoannis Imbert-Fernandez, Robert T. Spaulding, Jason Chesney, and Brian F. Clem
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Cell Survival ,Phosphofructokinase-2 ,medicine.drug_class ,Glucose uptake ,Estrogen receptor ,Apoptosis ,Breast Neoplasms ,Carbohydrate metabolism ,Biology ,Response Elements ,Biochemistry ,Breast cancer ,Fructosediphosphates ,medicine ,Humans ,Glycolysis ,Enzyme Inhibitors ,Fulvestrant ,Molecular Biology ,Estradiol ,Estrogen Receptor alpha ,Biological Transport ,Cell Biology ,Metabolism ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Glucose ,Estrogen ,Lymphatic Metastasis ,MCF-7 Cells ,Cancer research ,hormones, hormone substitutes, and hormone antagonists ,Intracellular - Abstract
Estradiol (E2) administered to estrogen receptor-positive (ER(+)) breast cancer patients stimulates glucose uptake by tumors. Importantly, this E2-induced metabolic flare is predictive of the clinical effectiveness of anti-estrogens and, as a result, downstream metabolic regulators of E2 are expected to have utility as targets for the development of anti-breast cancer agents. The family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) control glycolytic flux via their product, fructose-2,6-bisphosphate (F26BP), which activates 6-phosphofructo-1-kinase (PFK-1). We postulated that E2 might promote PFKFB3 expression, resulting in increased F26BP and glucose uptake. We demonstrate that PFKFB3 expression is highest in stage III lymph node metastases relative to normal breast tissues and that exposure of human MCF-7 breast cancer cells to E2 causes a rapid increase in [(14)C]glucose uptake and glycolysis that is coincident with an induction of PFKFB3 mRNA (via ER binding to its promoter), protein expression and the intracellular concentration of its product, F26BP. Importantly, selective inhibition of PFKFB3 expression and activity using siRNA or a PFKFB3 inhibitor markedly reduces the E2-mediated increase in F26BP, [(14)C]glucose uptake, and glycolysis. Furthermore, co-treatment of MCF-7 cells with the PFKFB3 inhibitor and the anti-estrogen ICI 182,780 synergistically induces apoptotic cell death. These findings demonstrate for the first time that the estrogen receptor directly promotes PFKFB3 mRNA transcription which, in turn, is required for the glucose metabolism and survival of breast cancer cells. Importantly, these results provide essential preclinical information that may allow for the ultimate design of combinatorial trials of PFKFB3 antagonists with anti-estrogen therapies in ER(+) stage IV breast cancer patients.
- Published
- 2014
18. Differential Dorso-ventral Distributions of Kv4.2 and HCN Proteins Confer Distinct Integrative Properties to Hippocampal CA1 Pyramidal Cell Distal Dendrites
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Zhiqiang Liu, Anne E. Anderson, Alan S. Lewis, Amy L. Brewster, Albert J. Becker, Shawn McClelland, Tallie Z. Baram, Joaquin N. Lugo, Monique Esclapez, Dane M. Chetkovich, Christophe Bernard, and Béatrice Marcelin
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Dorsum ,Potassium Channels ,Transcription, Genetic ,Cyclic Nucleotide-Gated Cation Channels ,Down-Regulation ,Hippocampus ,Nerve Tissue Proteins ,Dendrite ,Hippocampal formation ,Biology ,Biochemistry ,Ion Channels ,Neurobiology ,Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ,medicine ,Animals ,Molecular Biology ,Ion channel ,Pyramidal Cells ,Ca1 pyramidal neuron ,Dendrites ,Cell Biology ,Anatomy ,Potassium channel ,Rats ,Up-Regulation ,Electrophysiology ,Shal Potassium Channels ,medicine.anatomical_structure ,nervous system ,Organ Specificity ,Neuroscience - Abstract
The dorsal and ventral regions of the hippocampus perform different functions. Whether the integrative properties of hippocampal cells reflect this heterogeneity is unknown. We focused on dendrites where most synaptic input integration takes place. We report enhanced backpropagation and theta resonance and decreased summation of synaptic inputs in ventral versus dorsal CA1 pyramidal cell distal dendrites. Transcriptional Kv4.2 down-regulation and post-transcriptional hyperpolarization-activated cyclic AMP-gated channel (HCN1/2) up-regulation may underlie these differences, respectively. Our results reveal differential dendritic integrative properties along the dorso-ventral axis, reflecting diverse computational needs.
- Published
- 2012
19. Critical Role for Tetrahydrobiopterin Recycling by Dihydrofolate Reductase in Regulation of Endothelial Nitric-oxide Synthase Coupling
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Mark J. Crabtree, Keith M. Channon, Nicholas J. Alp, Amy L. Tatham, and Ashley B. Hale
- Subjects
Gene knockdown ,biology ,GTP cyclohydrolase I ,Biopterin ,Nitric Oxide Synthase Type III ,Cell Biology ,Tetrahydrobiopterin ,biology.organism_classification ,Biochemistry ,Nitric oxide ,Cell biology ,chemistry.chemical_compound ,chemistry ,Enos ,Dihydrofolate reductase ,biology.protein ,medicine ,Molecular Biology ,medicine.drug - Abstract
Tetrahyrobiopterin (BH4) is a required cofactor for the synthesis of nitric oxide by endothelial nitric-oxide synthase (eNOS), and BH4 bioavailability within the endothelium is a critical factor in regulating the balance between NO and superoxide production by eNOS (eNOS coupling). BH4 levels are determined by the activity of GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in de novo BH4 biosynthesis. However, BH4 levels may also be influenced by oxidation, forming 7,8-dihydrobiopterin (BH2), which promotes eNOS uncoupling. Conversely, dihydrofolate reductase (DHFR) can regenerate BH4 from BH2, but the functional importance of DHFR in maintaining eNOS coupling remains unclear. We investigated the role of DHFR in regulating BH4 versus BH2 levels in endothelial cells and in cell lines expressing eNOS combined with tet-regulated GTPCH expression in order to compare the effects of low or high levels of de novo BH4 biosynthesis. Pharmacological inhibition of DHFR activity by methotrexate or genetic knockdown of DHFR protein by RNA interference reduced intracellular BH4 and increased BH2 levels resulting in enzymatic uncoupling of eNOS, as indicated by increased eNOS-dependent superoxide but reduced NO production. In contrast to the decreased BH4:BH2 ratio induced by DHFR knockdown, GTPCH knockdown greatly reduced total biopterin levels but with no change in BH4:BH2 ratio. In cells expressing eNOS with low biopterin levels, DHFR inhibition or knockdown further diminished the BH4:BH2 ratio and exacerbated eNOS uncoupling. Taken together, these data reveal a key role for DHFR in eNOS coupling by maintaining the BH4:BH2 ratio, particularly in conditions of low total biopterin availability.
- Published
- 2009
20. Quantitative Regulation of Intracellular Endothelial Nitric-oxide Synthase (eNOS) Coupling by Both Tetrahydrobiopterin-eNOS Stoichiometry and Biopterin Redox Status
- Author
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Keith M. Channon, Shijie Cai, Mark J. Crabtree, Nicholas Warrick, Amy L. Tatham, Yasir Al-Wakeel, Ashley B. Hale, and Nicholas J. Alp
- Subjects
biology ,ATP synthase ,Superoxide ,GTP cyclohydrolase I ,Biopterin ,Cell Biology ,Tetrahydrobiopterin ,biology.organism_classification ,Biochemistry ,Cell biology ,chemistry.chemical_compound ,chemistry ,Cell culture ,Enos ,medicine ,biology.protein ,Molecular Biology ,Intracellular ,medicine.drug - Abstract
Tetrahydrobiopterin (BH4) is a critical determinant of endothelial nitric-oxide synthase (eNOS) activity. In the absence of BH4, eNOS becomes "uncoupled" and generates superoxide rather than NO. However, the stoichiometry of intracellular BH4/eNOS interactions is not well defined, and it is unclear whether intracellular BH4 deficiency alone is sufficient to induce eNOS uncoupling. To address these questions, we developed novel cell lines with tet-regulated expression of human GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in BH4 synthesis, to selectively induce intracellular BH4 deficiency by incubation with doxycycline. These cells were stably co-transfected to express a human eNOS-green fluorescent protein fusion protein, selecting clones expressing either low (GCH/eNOS-LOW) or high (GCH/eNOS-HIGH) levels. Doxycycline abolished GTPCH mRNA expression and GTPCH protein, leading to markedly diminished total biopterin levels and a decreased ratio of BH4 to oxidized biopterins in cells expressing eNOS. Intracellular BH4 deficiency induced superoxide generation from eNOS, as assessed by N-nitro-L-arginine methyl ester inhibitable 2-hydroxyethidium generation, and attenuated NO production. Quantitative analysis of cellular BH4 versus superoxide production between GCH/eNOS-LOW and GCH/eNOS-HIGH cells revealed a striking linear relationship between eNOS protein and cellular BH4 stoichiometry, with eNOS uncoupling at eNOS:BH4 molar ratio >1. Furthermore, increasing the intracellular BH2 concentration in the presence of a constant eNOS:BH4 ratio was sufficient to induce eNOS-dependent superoxide production. This specific, reductionist approach in a cell-based system reveals that eNOS:BH4 reaction stoichiometry together with the intracellular BH4:BH2 ratio, rather than absolute concentrations of BH4, are the key determinants of eNOS uncoupling, even in the absence of exogenous oxidative stress.
- Published
- 2009
21. Structural and Biochemical Characterization of the Oxidoreductase NmDsbA3 from Neisseria meningitidis
- Author
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Martin J. Scanlon, Yanni K.-Y. Chin, Jamie Rossjohn, Amy L. Robertson, Emma Byres, Stephen P. Bottomley, Jessica Scoullar, Travis Clarke Beddoe, Matthew C.J. Wilce, Julian P. Vivian, Charlene M. Kahler, Susannah Piek, James Horne, Jerome Wielens, Philip E. Thompson, and Tony Velkov
- Subjects
Protein Conformation ,Protein Disulfide-Isomerases ,Protein-disulfide reductase (glutathione) ,Neisseria meningitidis ,medicine.disease_cause ,Biochemistry ,Protein Structure, Secondary ,Substrate Specificity ,Microbiology ,Protein structure ,Bacterial Proteins ,Oxidoreductase ,Escherichia coli ,medicine ,Insulin ,Protein disulfide-isomerase ,Molecular Biology ,chemistry.chemical_classification ,biology ,Escherichia coli Proteins ,Membrane Proteins ,Active site ,Protein Disulfide Reductase (Glutathione) ,DNA ,Cell Biology ,Periplasmic space ,Recombinant Proteins ,Oxygen ,Dithiothreitol ,Kinetics ,DsbA ,chemistry ,biology.protein ,Oxidoreductases - Abstract
DsbA is an enzyme found in the periplasm of Gram-negative bacteria that catalyzes the formation of disulfide bonds in a diverse array of protein substrates, many of which are involved in bacterial pathogenesis. Although most bacteria possess only a single essential DsbA, Neisseria meningitidis is unusual in that it possesses three DsbAs, although the reason for this additional redundancy is unclear. Two of these N. meningitidis enzymes (NmDsbA1 and NmDsbA2) play an important role in meningococcal attachment to human epithelial cells, whereas NmDsbA3 is considered to have a narrow substrate repertoire. To begin to address the role of DsbAs in the pathogenesis of N. meningitidis, we have determined the structure of NmDsbA3 to 2.3-A resolution. Although the sequence identity between NmDsbA3 and other DsbAs is low, the NmDsbA3 structure adopted a DsbA-like fold. Consistent with this finding, we demonstrated that NmDsbA3 acts as a thiol-disulfide oxidoreductase in vitro and is reoxidized by Escherichia coli DsbB (EcDsbB). However, pronounced differences in the structures between DsbA3 and EcDsbA, which are clustered around the active site of the enzyme, suggested a structural basis for the unusual substrate specificity that is observed for NmDsbA3.
- Published
- 2008
22. A Block in Endoplasmic Reticulum-to-Golgi Trafficking Inhibits Phospholipid Synthesis and Induces Neutral Lipid Accumulation
- Author
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Sepp D. Kohlwein, Maria L. Gaspar, Susan A. Henry, William J. Brown, Raghuvir Viswanatha, Stephen A. Jesch, and Amy L. Antosh
- Subjects
Protein Denaturation ,Time Factors ,Saccharomyces cerevisiae ,Golgi Apparatus ,Lipids and Lipoproteins: Metabolism, Regulation, and Signaling ,Vacuole ,Endoplasmic Reticulum ,Models, Biological ,Biochemistry ,Cell membrane ,symbols.namesake ,Lipid droplet ,medicine ,Molecular Biology ,Phospholipids ,Triglycerides ,biology ,Endoplasmic reticulum ,Cell Membrane ,Temperature ,Biological Transport ,Lipid metabolism ,Cell Biology ,Golgi apparatus ,Flow Cytometry ,biology.organism_classification ,Lipids ,Membrane contact site ,Cell biology ,Microscopy, Electron ,medicine.anatomical_structure ,symbols ,lipids (amino acids, peptides, and proteins) - Abstract
Seeking to better understand how membrane trafficking is coordinated with phospholipid synthesis in yeast, we investigated lipid synthesis in several Sec(-) temperature-sensitive mutants, including sec13-1. Upon shift of sec13-1 cells to the restrictive temperature of 37 degrees C, phospholipid synthesis decreased dramatically relative to the wild type control, whereas synthesis of neutral lipids, especially triacylglycerol (TAG), increased. When examined by fluorescence microscopy, the number of lipid droplets appeared to increase and formed aggregates in sec13-1 cells shifted to 37 degrees C. Electron microscopy confirmed the increase in lipid droplet number and revealed that many were associated with the vacuole. Analysis of lipid metabolism in strains lacking TAG synthase genes demonstrated that the activities of the products of these genes contribute to accumulation of TAG in sec13-1 cells after the shift to 37 degrees C. Furthermore, the permissive temperature for growth of the sec13-1 strain lacking TAG synthase genes was 3 degrees C lower than sec13-1 on several different growth media, indicating that the synthesis of TAG has physiological significance under conditions of secretory stress. Together these results suggest that following a block in membrane trafficking, yeast cells channel lipid metabolism from phospholipid synthesis into synthesis of TAG and other neutral lipids to form lipid droplets. We conclude that this metabolic switch provides a degree of protection to cells during secretory stress.
- Published
- 2008
23. Laminar Shear Stress Up-regulates Peroxiredoxins (PRX) in Endothelial Cells
- Author
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Amy L. Mowbray, Hanjoong Jo, Dong Hoon Kang, Sue Goo Rhee, and Sang Won Kang
- Subjects
chemistry.chemical_classification ,Reactive oxygen species ,medicine.diagnostic_test ,Cell Biology ,Anatomy ,Mitochondrion ,Biology ,Subcellular localization ,medicine.disease_cause ,Biochemistry ,Cell biology ,Endothelial stem cell ,chemistry ,Downregulation and upregulation ,Western blot ,medicine ,Mechanosensitive channels ,Molecular Biology ,Oxidative stress - Abstract
Shear stress plays a significant role in endothelial cell biology and atherosclerosis development. Previous work by our group has shown that fluid flow stimulates important functional changes in cells through protein expression regulation. Peroxiredoxins (PRX) are a family of antioxidant enzymes but have yet to be investigated in response to shear stress. Studies have shown that oscillatory shear stress (OS) increases reactive oxygen species (ROS) levels in endothelial cells, whereas laminar shear stress (LS) blocks this response. We hypothesized that PRX are responsible for the anti-oxidative effect of LS. To test this hypothesis, bovine aortic endothelial cells (BAEC) were subjected to LS (15 dyn/cm2), OS (±5 dyn/cm2, 1 Hz), or static conditions for 24 h. Using Western blot and immunofluorescence staining, all six isoforms of PRX were identified in BAEC. When compared with OS and static, exposure to chronic LS up-regulated PRX 1 levels intracellularly. LS also increased expression of PRX 5 relative to static controls, but not OS. PRX exhibited broad subcellular localization, with distribution in the cytoplasm, Golgi, mitochondria, and intermediate filaments. In addition, PRX 1 knock down, using specific small interference RNA, attenuated LS-dependent reactive oxygen species reduction in BAEC. However, PRX 5 depletion did not. Together, these results suggest that PRX 1 is a novel mechanosensitive antioxidant, playing an important role in shear-dependent regulation of endothelial biology and atherosclerosis.
- Published
- 2008
24. Specific Reduction of Hepatic Glucose 6-Phosphate Transporter-1 Ameliorates Diabetes while Avoiding Complications of Glycogen Storage Disease
- Author
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Angela M. Siesky, Eleftheria N. Finger, Kyle W. Sloop, Brett P. Monia, Armando R. Irizarry, Amy L. Cox, Robert Mckay, Sanjay Bhanot, Susan F. Murray, M. Dodson Michael, Hong Yan Zhang, Aaron D. Showalter, Sheri L. Booten, and Julia X.C. Cao
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,Monosaccharide Transport Proteins ,Mice, Obese ,Hyperlipidemias ,Hyperuricemia ,Type 2 diabetes ,Biology ,Hypoglycemia ,Kidney ,Biochemistry ,Antiporters ,Diabetes Complications ,Mice ,chemistry.chemical_compound ,Internal medicine ,Diabetes mellitus ,medicine ,Animals ,Glycogen storage disease ,RNA, Messenger ,Molecular Biology ,Mice, Knockout ,Lipid metabolism ,Cell Biology ,Carbohydrate ,Glucagon ,Glycogen Storage Disease ,medicine.disease ,Mice, Inbred C57BL ,Endocrinology ,Diabetes Mellitus, Type 2 ,Liver ,chemistry ,Glycogenesis ,Glucose-6-Phosphatase ,Uric acid ,Acidosis, Lactic ,Glycogen ,Oligoribonucleotides, Antisense - Abstract
d-Glucose-6-phosphatase is a key regulator of endogenous glucose production, and its inhibition may improve glucose control in type 2 diabetes. Herein, 2′-O-(2-methoxy)ethyl-modified phosphorothioate antisense oligonucleotides (ASOs) specific to the glucose 6-phosphate transporter-1 (G6PT1) enabled reduction of hepatic d-Glu-6-phosphatase activity in diabetic ob/ob mice. Treatment with G6PT1 ASOs decreased G6PT1 expression, reduced G6PT1 activity, blunted glucagon-stimulated glucose production, and lowered plasma glucose concentration in a dose-dependent manner. In contrast to G6PT1 knock-out mice and patients with glycogen storage disease, excess hepatic and renal glycogen accumulation, hyperlipidemia, neutropenia, and elevations in plasma lactate and uric acid did not occur. In addition, hypoglycemia was not observed in animals during extended periods of fasting, and the ability of G6PT1 ASO-treated mice to recover from an exogenous insulin challenge was not impaired. Together, these results demonstrate that effective glucose lowering by G6PT1 inhibitors can be achieved without adversely affecting carbohydrate and lipid metabolism.
- Published
- 2007
25. Disease-associated Mutations at Copper Ligand Histidine Residues of Superoxide Dismutase 1 Diminish the Binding of Copper and Compromise Dimer Stability
- Author
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Jonathan P. Scheurmann, Xiaohang Cao, Jiou Wang, Angela Rodriguez, David R. Borchelt, Jonathan D. Gitlin, Hilda H. Slunt, P. John Hart, and Amy L. Caruano-Yzermans
- Subjects
Models, Molecular ,Mutant ,SOD1 ,chemistry.chemical_element ,Mice, Transgenic ,Plasma protein binding ,Ligands ,Biochemistry ,Article ,Superoxide dismutase ,Mice ,Two-Hybrid System Techniques ,Animals ,Humans ,Histidine ,Molecular Biology ,Neurons ,biology ,Superoxide Dismutase ,Chemistry ,Ligand ,Active site ,Cell Biology ,Copper ,Mutation ,biology.protein ,Dimerization ,Protein Binding - Abstract
A subset of superoxide dismutase 1 (Cu/Zn-SOD1) mutants that cause familial amyotrophic lateral sclerosis (FALS) have heightened reactivity with (-)ONOO and H(2)O(2) in vitro. This reactivity requires a copper ion bound in the active site and is a suggested mechanism of motor neuron injury. However, we have found that transgenic mice that express SOD1-H46R/H48Q, which combines natural FALS mutations at ligands for copper and which is inactive, develop motor neuron disease. Using a direct radioactive copper incorporation assay in transfected cells and the established tools of single crystal x-ray diffraction, we now demonstrate that this variant does not stably bind copper. We find that single mutations at copper ligands, including H46R, H48Q, and a quadruple mutant H46R/H48Q/H63G/H120G, also diminish the binding of radioactive copper. Further, using native polyacrylamide gel electrophoresis and a yeast two-hybrid assay, the binding of copper was found to be related to the formation of the stable dimeric enzyme. Collectively, our data demonstrate a relationship between copper and assembly of SOD1 into stable dimers and also define disease-causing SOD1 mutants that are unlikely to robustly produce toxic radicals via copper-mediated chemistry.
- Published
- 2007
26. Farnesoid X Receptor Agonist Reduces Serum Asymmetric Dimethylarginine Levels through Hepatic Dimethylarginine Dimethylaminohydrolase-1 Gene Regulation
- Author
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Amy L. Cox, Timothy P. Ryan, Michael L. Chouinard, James Ficorilli, M. Dodson Michael, Laura F. Michael, Marialuisa C. Marcelo, Hong Gao, Philip Sipes, Tonghuan Hu, and Shuyu Li
- Subjects
Agonist ,medicine.medical_specialty ,medicine.drug_class ,Response element ,Receptors, Cytoplasmic and Nuclear ,Biology ,Arginine ,Biochemistry ,Amidohydrolases ,Cell Line ,chemistry.chemical_compound ,Internal medicine ,Gene expression ,medicine ,Transcriptional regulation ,Animals ,Humans ,Molecular Biology ,Regulation of gene expression ,Dose-Response Relationship, Drug ,Isoxazoles ,Cell Biology ,Rats ,Rats, Zucker ,DNA-Binding Proteins ,Endocrinology ,Gene Expression Regulation ,Liver ,chemistry ,Nuclear receptor ,Female ,Farnesoid X receptor ,Asymmetric dimethylarginine ,Transcription Factors - Abstract
The farnesoid X receptor (FXR, NR1H4) is a bile acid-responsive nuclear receptor that plays critical roles in the transcriptional regulation genes involved in cholesterol, bile acid, triglyceride, and carbohydrate metabolism. By microarray analysis of hepatic genes from female Zucker diabetic fatty (ZDF) rats treated with the FXR agonist GW4064, we have identified dimethylarginine dimethylaminohydrolase-1 (DDAH1) as an FXR target gene. DDAH1 is a key catabolic enzyme of asymmetric dimethylarginine (ADMA), a major endogenous nitric-oxide synthase inhibitor. Sequence analysis of the DDAH1 gene reveals the presence of an FXR response element (FXRE) located 90 kb downstream of the transcription initiation site and within the first intron. Functional analysis of the putative FXRE demonstrated GW4064 dose-dependent transcriptional activation from the element, and we have demonstrated that the FXRE sequence binds the FXR-RXR heterodimer. In vivo administration of GW4064 to female ZDF rats promoted a dose-dependent and >6-fold increase in hepatic DDAH1 gene expression. The level of serum ADMA was reduced concomitantly. These findings provide a mechanism by which FXR may increase endothelium-derived nitric oxide levels through modulation of serum ADMA levels via direct regulation of hepatic DDAH1 gene expression. Thus, beneficial clinical outcomes of FXR agonist therapy may include prevention of atherosclerosis and improvement of the metabolic syndrome.
- Published
- 2006
27. Ca2+ Dysregulation Induces Mitochondrial Depolarization and Apoptosis
- Author
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Amy L. Howes, Shigeki Miyamoto, Joan Heller Brown, John W. Adams, and Gerald W. Dorn
- Subjects
Membrane potential ,Cytosol ,Mitochondrial permeability transition pore ,Sodium-calcium exchanger ,Apoptosis ,Endoplasmic reticulum ,Depolarization ,Cell Biology ,Mitochondrion ,Biology ,Molecular Biology ,Biochemistry ,Cell biology - Abstract
We previously reported that constitutively activated Gαq (Q209L) expression in cardiomyocytes induces apoptosis through opening of the mitochondrial permeability transition pore. We assessed the hypothesis that disturbances in Ca2+ handling linked Gαq activity to apoptosis because resting Ca2+ levels were significantly increased prior to development of apoptosis. Treating cells with EGTA lowered Ca2+ and blocked both loss of mitochondrial membrane potential (an indicator of permeability transition pore opening) and apoptosis (assessed by DNA fragmentation). When cytosolic Ca2+ and mitochondrial membrane potential were simultaneously measured by confocal microscopy, sarcoplasmic reticulum (SR)-driven slow Ca2+ oscillations (time-to-peak ∼4 s) were observed in Q209L-expressing cells. These oscillations were seen to transition into sustained increases in cytosolic Ca2+, directly paralleled by loss of mitochondrial membrane potential. Ca2+ transients generated by caffeine-induced release of SR Ca2+ were greatly prolonged in Q209L-expressing cells, suggesting a decreased ability to extrude Ca2+. Indeed, the Na+/Ca2+ exchanger (NCX), which removes Ca2+ from the cell, was markedly down-regulated at the mRNA and protein levels. Adenoviral NCX expression normalized cytosolic Ca2+ levels and prevented DNA fragmentation in cells expressing Q209L. Interestingly, constitutively activated Akt, which rescues cells from Q209L-induced apoptosis, prevented the decrease in NCX expression, normalized cytosolic Ca2+ levels and spontaneous Ca2+ oscillations, shortened caffeine-induced Ca2+ transients, and prevented loss of the mitochondrial membrane potential. Our findings demonstrate that NCX down-regulation and consequent increases in cytosolic and SR Ca2+ can lead to Ca2+ overloading-induced loss of mitochondrial membrane potential and suggest that recovery of Ca2+ dysregulation is a target of Akt-mediated protection.
- Published
- 2005
28. μ and κ Opioid Receptors Activate ERK/MAPK via Different Protein Kinase C Isoforms and Secondary Messengers in Astrocytes
- Author
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Alexi Kiss, Amy L. Clark, Jannie S. Serna, Mariana M. Belcheva, Jason W. Hahn, Paul D. Haas, and Carmine J. Coscia
- Subjects
MAPK/ERK pathway ,Indoles ,Pyrrolidines ,Enkephalin ,Benzeneacetamides ,Receptors, Opioid, mu ,Biochemistry ,Maleimides ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,Protein Isoforms ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Cells, Cultured ,Protein Kinase C ,Neurons ,Analgesics ,Cell biology ,Analgesics, Opioid ,ErbB Receptors ,DAMGO ,Second messenger system ,Signal transduction ,Protein Binding ,Signal Transduction ,MAP Kinase Signaling System ,Immunoblotting ,Carbazoles ,Protein Kinase C-epsilon ,Biology ,Transfection ,Article ,Calmodulin ,Animals ,Humans ,Immunoprecipitation ,Molecular Biology ,Protein kinase C ,Phospholipase C ,Receptors, Opioid, kappa ,Cell Membrane ,Cell Biology ,Enkephalin, Ala(2)-MePhe(4)-Gly(5) ,Molecular biology ,Rats ,Enzyme Activation ,chemistry ,Astrocytes ,Type C Phospholipases ,Calcium - Abstract
Acute mu and kappa opioids activate the ERK/MAPK phosphorylation cascade that represents an integral part of the signaling pathway of growth factors in astrocytes. By this cross-talk, opioids may impact neural development and plasticity among other basic neurobiological processes in vivo. The mu agonist, [D-ala2,mephe4,glyol5]enkephalin (DAMGO), induces a transient stimulation of ERK phosphorylation, whereas kappa agonist, U69,593, engenders sustained ERK activation. Here we demonstrate that acute U69,593 and DAMGO stimulate ERK phosphorylation by utilization of different secondary messengers and protein kinase C (PKC) isoforms upstream of the growth factor pathway. Immortalized astrocytes transfected with either antisense calmodulin (CaM), a mutant mu opioid receptor that binds CaM poorly or a dominant negative mutant of PKCepsilon were used as a model system to study mu signaling. Evidence was gained to implicate CaM and PKCepsilon in DAMGO stimulation of ERK. DAMGO activation of PKCepsilon and/or ERK was insensitive to selective inhibitors of Ca2+ mobilization, but it was blocked upon phospholipase C inhibition. These results suggest a novel mechanism wherein, upon DAMGO binding, CaM is released from the mu receptor and activates phospholipase C. Subsequently, phospholipase C generates diacylglycerides that activate PKCepsilon. In contrast, U69,593 appears to act via phosphoinositide 3-kinase, PKCzeta, and Ca2+ mobilization. These signaling components were implicated based on studies with specific inhibitors and a dominant negative mutant of PKCzeta. Collectively, our findings on acute opioid effects suggest that differences in their mechanism of signaling may contribute to the distinct outcomes on ERK modulation induced by chronic mu and kappa opioids.
- Published
- 2005
29. Myosin II Regulatory Light Chain Is Required for Trafficking of Bile Salt Export Protein to the Apical Membrane in Madin-Darby Canine Kidney Cells
- Author
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Wayne Chan, German Calderon, Hiroshi Hosoya, Amy L. Swift, Jamie Moseley, Irwin M. Arias, Shaohua Li, and Daniel F. Ortiz
- Subjects
Immunoprecipitation ,Recombinant Fusion Proteins ,ATP-binding cassette transporter ,Plasma protein binding ,Biology ,Kidney ,Biochemistry ,Cell Line ,Bile Acids and Salts ,Dogs ,Myosin ,Animals ,Secretion ,Phosphorylation ,Molecular Biology ,Myosin Type II ,Bile Canaliculi ,Kidney metabolism ,Cell Biology ,Apical membrane ,Molecular biology ,Transport protein ,Protein Transport ,Microscopy, Fluorescence ,Carrier Proteins ,Plasmids ,Protein Binding - Abstract
BSEP, MDR1, and MDR2 ATP binding cassette transporters are targeted to the apical (canalicular) membrane of hepatocytes, where they mediate ATP-dependent secretion of bile acids, drugs, and phospholipids, respectively. Sorting to the apical membrane is essential for transporter function; however, little is known regarding cellular proteins that bind ATP binding cassette proteins and regulate their trafficking. A yeast two-hybrid screen of a rat liver cDNA library identified the myosin II regulatory light chain, MLC2, as a binding partner for BSEP, MDR1, and MDR2. The interactions were confirmed by glutathione S-transferase pulldown and co-immunoprecipitation assays. BSEP and MLC2 were overrepresented in a rat liver subcellular fraction enriched in canalicular membrane vesicles, and MLC2 colocalized with BSEP in the apical domain of hepatocytes and polarized WifB, HepG2, and Madin-Darby canine kidney cells. Expression of a dominant negative, non-phosphorylatable MLC2 mutant reduced steady state BSEP levels in the apical domain of polarized Madin-Darby canine kidney cells. Pulse-chase studies revealed that Blebbistatin, a specific myosin II inhibitor, severely impaired delivery of newly synthesized BSEP to the apical surface. These findings indicate that myosin II is required for BSEP trafficking to the apical membrane.
- Published
- 2005
30. Maltose-binding Protein Is Open in the Catalytic Transition State for ATP Hydrolysis during Maltose Transport
- Author
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Mariana I. Austermuhle, Candice S. Klug, Amy L. Davidson, and Jason A. Hall
- Subjects
Models, Molecular ,Protein Conformation ,Protein subunit ,Ligands ,Models, Biological ,Biochemistry ,Catalysis ,Maltose-Binding Proteins ,Maltose-binding protein ,Adenosine Triphosphate ,Protein structure ,ATP hydrolysis ,Escherichia coli ,Cysteine ,Cloning, Molecular ,Maltose ,Spin label ,Molecular Biology ,Maltose transport ,Chromatography ,biology ,Chemistry ,Hydrolysis ,X-Rays ,Electron Spin Resonance Spectroscopy ,Biological Transport ,Cell Biology ,Site-directed spin labeling ,Protein Structure, Tertiary ,Periplasm ,Mutagenesis, Site-Directed ,biology.protein ,Spin Labels ,Vanadates ,Carrier Proteins ,Maltose transport complex ,Protein Binding - Abstract
The maltose transport complex of Escherichia coli, a member of the ATP-binding cassette superfamily, mediates the high affinity uptake of maltose at the expense of ATP. The membrane-associated transporter consists of two transmembrane subunits, MalF and MalG, and two copies of the cytoplasmic ATP-binding cassette subunit, MalK. Maltose-binding protein (MBP), a soluble periplasmic protein, delivers maltose to the MalFGK(2) transporter and stimulates hydrolysis by the transporter. Site-directed spin labeling electron paramagnetic resonance spectroscopy is used to monitor binding of MBP to MalFGK(2) and conformational changes in MBP as it interacts with MalFGK(2). Cysteine residues and spin labels have been introduced into the two lobes of MBP so that spin-spin interaction will report on ligand-induced closure of the protein (Hall, J. A., Thorgeirsson, T. E., Liu, J., Shin, Y. K., and Nikaido, H. (1997) J. Biol. Chem. 272, 17610-17614). At least two different modes of interaction between MBP and MalFGK(2) were detected. Binding of MBP to MalFGK(2) in the absence of ATP resulted in a decrease in motion of spin label at position 41 in the C-terminal domain of MBP. In a vanadate-trapped transition state intermediate, all free MBP became tightly bound to MalFGK(2), spin label in both lobes became completely immobilized, and spin-spin interactions were lost, suggesting that MBP was in an open conformation. Binding of non-hydrolyzable MgATP analogs or ATP in the absence of Mg is sufficient to stabilize a complex of open MBP and MalFGK(2). Taken together, these data suggest that closure of the MalK dimer interface coincides with opening of MBP and maltose release to the transporter.
- Published
- 2004
31. Identification of HAX-1 as a Protein That Binds Bile Salt Export Protein and Regulates Its Abundance in the Apical Membrane of Madin-Darby Canine Kidney Cells
- Author
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Amy L. Swift, German Calderon, Shaohua Li, Irwin M. Arias, James Moseley, and Daniel F. Ortiz
- Subjects
Time Factors ,ATP-binding cassette transporter ,Biochemistry ,chemistry.chemical_compound ,Internalization ,ATP Binding Cassette Transporter, Subfamily B, Member 11 ,Phospholipids ,Genes, Dominant ,Glutathione Transferase ,media_common ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Vesicle ,Multidrug resistance-associated protein 2 ,Microfilament Proteins ,Cell biology ,Protein Transport ,Liver ,RNA Interference ,Cortactin ,Plasmids ,Protein Binding ,Subcellular Fractions ,ATP Binding Cassette Transporter, Subfamily B ,media_common.quotation_subject ,Immunoblotting ,Molecular Sequence Data ,Transfection ,Models, Biological ,Cell Line ,Bile Acids and Salts ,Dogs ,Cations ,Two-Hybrid System Techniques ,Escherichia coli ,Animals ,Humans ,Biotinylation ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Amino Acid Sequence ,Molecular Biology ,Adaptor Proteins, Signal Transducing ,Sequence Homology, Amino Acid ,Cell Membrane ,Proteins ,Biological Transport ,Transporter ,Cell Biology ,Glutathione ,Apical membrane ,Precipitin Tests ,Rats ,Microscopy, Fluorescence ,chemistry ,Protein Biosynthesis ,Hepatocytes ,biology.protein ,ATP-Binding Cassette Transporters ,Protein Processing, Post-Translational - Abstract
ATP-binding cassette (ABC)-type proteins are essential for bile formation in vertebrate liver. BSEP, MDR1, MDR2, and MRP2 ABC transporters are targeted to the apical (canalicular) membrane of hepatocytes where they execute ATP-dependent transport of bile acids, drugs, amphipathic cations, phospholipids, and conjugated organic anions, respectively. Changes in activity and abundance of transporters in the canalicular membrane regulate bile flow; however, little is known regarding cellular proteins that bind ABC transporters and regulate their trafficking. A yeast two-hybrid screen identified HAX-1 as a binding partner for BSEP, MDR1, and MDR2. The interactions were validated biochemically by glutathione S-transferase pull-down and co-immunoprecipitation assays. BSEP and HAX-1 were over-represented in rat liver subcellular fractions enriched for canalicular membrane vesicles, microsomes, and clathrin-coated vesicles. HAX-1 was bound to BSEP, MDR1, and MDR2 in canalicular membrane vesicles and co-localized with BSEP and MDR1 in the apical membrane of Madin-Darby canine kidney (MDCK) cells. RNA interference of HAX-1 increased BSEP levels in the apical membrane of MDCK cells by 71%. Pulse-chase studies indicated that HAX-1 depletion did not affect BSEP translation, post-translational modification, delivery to the plasma membrane, or half-life. HAX-1 depletion resulted in an increased peak of metabolically labeled apical membrane BSEP at 4 h and enhanced retention at 6 and 9 h. HAX-1 also interacts with cortactin. Expression of dominant negative cortactin increased steady state levels of BSEP 2-fold in the apical membrane of MDCK cells, as did expression of dominant negative EPS15. These findings suggest that HAX-1 and cortactin participate in BSEP internalization from the apical membrane.
- Published
- 2004
32. PE-1/METS, an Antiproliferative Ets Repressor Factor, Is Induced by CREB-1/CREM-1 during Macrophage Differentiation
- Author
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Stephan Herzig, Laure Escoubet-Lozach, Christopher K. Glass, Amy L. Fong, Dominique Sawka-Verhelle, Patricia Lebrun, and Kelly D. Hester
- Subjects
Time Factors ,Cell ,Biochemistry ,Mice ,Cloning, Molecular ,Phosphorylation ,Cyclic AMP Response Element-Binding Protein ,Promoter Regions, Genetic ,Genes, Dominant ,Oncogene Proteins ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Differentiation ,U937 Cells ,Flow Cytometry ,Intercellular Adhesion Molecule-1 ,Chromatin ,Up-Regulation ,Cell biology ,DNA-Binding Proteins ,medicine.anatomical_structure ,Cell Division ,Blotting, Western ,Molecular Sequence Data ,Antigens, Differentiation, Myelomonocytic ,Repressor ,Bone Marrow Cells ,Biology ,Transfection ,CREB ,Adenoviridae ,Cyclic AMP Response Element Modulator ,Antigens, CD ,Cell Adhesion ,medicine ,Animals ,Humans ,Cell adhesion ,Molecular Biology ,Transcription factor ,Macrosialin ,Cell Nucleus ,Base Sequence ,Models, Genetic ,Proto-Oncogene Proteins c-ets ,Macrophages ,Promoter ,DNA ,Cell Biology ,Precipitin Tests ,Molecular biology ,Protein Structure, Tertiary ,Repressor Proteins ,Mutation ,biology.protein ,Chromatin immunoprecipitation ,Transcription Factors - Abstract
The molecular mechanisms involved in regulating the balance between cellular proliferation and differentiation remain poorly understood. Members of the Ets-domain family of transcription factors are candidates for proteins that might differentially regulate cell cycle control and cell type-specific genes during the differentiation of myeloid progenitor cells. The Ets repressor PE-1/METS has been suggested to contribute to growth arrest during terminal macrophage differentiation by repressing Ets target genes involved in Ras-dependent proliferation. An important feature of this regulatory model is that PE-1/METS is itself induced by the program of macrophage differentiation elicited by M-CSF. Here, we present evidence that the PE-1/METS gene is a transcriptional target of the cyclic AMP response element-binding protein-1 (CREB-1). CREB-1 expression is dramatically up-regulated during macrophage differentiation and phosphorylation of CREB-1 and the related factor CREM-1 are stimulated by M-CSF in a SAPK2/p38-dependent manner. Chromatin immunoprecipitation experiments demonstrate that CREB-1/CREM-1 are recruited to the PE-1/METS promoter as well as to the promoters of other genes that are up-regulated during terminal macrophage differentiation. Overexpression of CREB-1 stimulates the activities of the PE-1/METS, and macrosialin promoters, while expression of a dominant negative form of CREB-1 during macrophage differentiation inhibits expression of the PE-1/METS and macrosialin genes. Inhibition of CREB function also results in reduced expression of CD54 and impaired cell adhesion. Taken together, these findings reveal new roles of CREB-1/CREM-1 as regulators of macrophage differentiation.
- Published
- 2004
33. Disulfide Cross-linking Reveals a Site of Stable Interaction between C-terminal Regulatory Domains of the Two MalK Subunits in the Maltose Transport Complex
- Author
-
Amy L. Davidson, Susmita Samanta, Howard A. Shuman, Moriama Reyes, Jue Chen, and Tulin Ayvaz
- Subjects
Models, Molecular ,Monosaccharide Transport Proteins ,Protein Conformation ,Stereochemistry ,Dimer ,ATP-binding cassette transporter ,Biochemistry ,Protein Structure, Secondary ,chemistry.chemical_compound ,Bacterial Proteins ,ATP hydrolysis ,Nucleotide ,Disulfides ,Maltose ,Molecular Biology ,DNA Primers ,Adenosine Triphosphatases ,chemistry.chemical_classification ,Base Sequence ,Escherichia coli Proteins ,Walker motifs ,Cell Biology ,Protein Subunits ,A-site ,chemistry ,Mutagenesis, Site-Directed ,ATP-Binding Cassette Transporters ,Maltose transport complex ,Plasmids ,Cysteine - Abstract
Understanding the structure and function of the ATP-binding cassette (ABC) transporters is very important because defects in ABC transporters lie at the root of several serious diseases including cystic fibrosis. MalK, the ATP-binding cassette of the maltose transporter of Escherichia coli, is distinct from most other ATP-binding cassettes in that it contains an additional C-terminal regulatory domain. The published structure of a MalK dimer is elongated with C-terminal domains at opposite poles (Diederichs, K., Diez, J., Greller, G., Muller, C., Breed, J., Schnell, C., Vonrhein, C., Boos, W., and Welte, W. (2000) EMBO J. 19, 5951-5961). Some uncertainty exists as to whether the orientation of MalK in the dimer structure is correct. Superpositioning of the N-terminal domains of MalK onto the ATP-binding domains of an alternate ABC dimer, in which ATP is bound along the dimer interface between Walker A and LSGGQ motifs, places both N- and C-terminal domains of MalK along the dimer interface. Consistent with this model, a cysteine substitution at position 313 in the C-terminal domain of an otherwise cysteine-free MalK triggered disulfide bond formation between two MalK subunits in an intact maltose transporter. Disulfide bond formation did not inhibit the function of the transporter, suggesting that the C-terminal domains of MalK remain in close proximity throughout the transport cycle. Enzyme IIAglc still inhibited the ATPase activity of the disulfide-linked transporter indicating that the mechanism of inducer exclusion was unaffected. These data support a model for ATP hydrolysis in which the C-terminal domains of MalK remain in contact whereas the N-terminal domains of MalK open and close to allow nucleotide binding and dissociation.
- Published
- 2003
34. Receptors Involved in the Oxidized 1-Palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine-mediated Synthesis of Interleukin-8
- Author
-
Nima M. Gharavi, Grace Wang, Judith A. Berliner, Xavier Hsieh, Kimberly A. Walton, Shirley Wang, Michael W. Yeh, and Amy L. Cole
- Subjects
Receptor complex ,Testicular receptor 4 ,Liver receptor homolog-1 ,Cell Biology ,Biology ,Biochemistry ,Macrophage receptor with collagenous structure ,Enzyme-linked receptor ,lipids (amino acids, peptides, and proteins) ,5-HT5A receptor ,Molecular Biology ,Coagulation factor II receptor ,Protease-activated receptor 2 - Abstract
We demonstrated previously that oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (ox-PAPC) and, specifically, the component lipid 1-palmitoyl-2-(5,6-epoxyisoprostane E2)-sn-glycero-3-phosphorylcholine increase interleukin-8 (IL-8) synthesis in aortic endothelial cells. The goal of the current studies was to characterize the receptor complex mediating the increased transcription of IL-8. We demonstrate that scavenger receptor class A, types I and II, lectin-like ox-LDL receptor-1, macrophage receptor with collagenous structure, and CD36 are not responsible for the increase in IL-8. Using dominant-negative constructs and antisense oligonucleotides, we demonstrate a role for Toll-like receptor 4 (TLR4) as the ox-PAPC receptor mediating IL-8 transcription. We demonstrate that a glycosylphosphatidylinositol (GPI)-anchored protein is also necessary because phosphatidylinositol-specific phospholipase C pretreatment inhibited the effect of ox-PAPC. CD14, a GPI-anchored protein that associates with TLR4 in mediating lipopolysaccharide action, did not appear to mediate ox-PAPC action because ox-PAPC-induced IL-8 transcription was not blocked by anti-CD14 neutralizing antibodies nor was it augmented by the addition of soluble CD14 or overexpression of membrane CD14. Instead, anti-TLR4 antibodies immunoprecipitated a 37-kDa protein that also bound ox-PAPC. A protein of this same size was found in aerolysin overlays used to detect GPI-anchored proteins. Therefore, these studies suggest that ox-PAPC may initially bind to a 37-kDa GPI-anchored protein, which interacts with TLR4 to induce IL-8 transcription.
- Published
- 2003
35. Inhibition of Hepatitis C Virus RNA Replication by 2′-Modified Nucleoside Analogs
- Author
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Malcolm MacCoss, Amy L. Simcoe, Bohdan S. Wolanski, Raffaele De Francesco, Balkrishen Bhat, David B. Olsen, Giovanni Migliaccio, Carrie A. Rutkowski, Mark Stahlhut, Lawrence C. Kuo, Zhucheng Yang, Dawn L. Hall, Joanne E. Tomassini, Krista Getty, Robert L. Lafemina, Anne B. Eldrup, Michele Bosserman, and Steven S. Carroll
- Subjects
Adenosine ,Cytidine Triphosphate ,viruses ,Hepatitis C virus ,RNA-dependent RNA polymerase ,Cytidine ,DNA-Directed DNA Polymerase ,Hepacivirus ,Viral Nonstructural Proteins ,Biology ,Virus Replication ,medicine.disease_cause ,Biochemistry ,chemistry.chemical_compound ,Adenosine Triphosphate ,RNA polymerase ,medicine ,Humans ,Replicon ,Molecular Biology ,NS5B ,Cells, Cultured ,DNA Polymerase beta ,Nucleic Acid Synthesis Inhibitors ,virus diseases ,RNA ,Cell Biology ,DNA Polymerase I ,Hepatitis C ,Virology ,digestive system diseases ,DNA Polymerase gamma ,NS2-3 protease ,chemistry ,Nucleoside triphosphate ,RNA, Viral ,Gels - Abstract
The RNA-dependent RNA polymerase (NS5B) of hepatitis C virus (HCV) is essential for the replication of viral RNA and thus constitutes a valid target for the chemotherapeutic intervention of HCV infection. In this report, we describe the identification of 2'-substituted nucleosides as inhibitors of HCV replication. The 5'-triphosphates of 2'-C-methyladenosine and 2'-O-methylcytidine are found to inhibit NS5B-catalyzed RNA synthesis in vitro, in a manner that is competitive with substrate nucleoside triphosphate. NS5B is able to incorporate either nucleotide analog into RNA as determined with gel-based incorporation assays but is impaired in its ability to extend the incorporated analog by addition of the next nucleotide. In a subgenomic replicon cell line, 2-C-methyladenosine and 2'-O-methylcytidine inhibit HCV RNA replication. The 5'-triphosphates of both nucleosides are detected intracellularly following addition of the nucleosides to the media. However, significantly higher concentrations of 2'-C-methyladenosine triphosphate than 2'-O-methylcytidine triphosphate are detected, consistent with the greater potency of 2'-C-methyladenosine in the replicon assay, despite similar inhibition of NS5B by the triphosphates in the in vitro enzyme assays. Thus, the 2'-modifications of natural substrate nucleosides transform these molecules into potent inhibitors of HCV replication.
- Published
- 2003
36. Targeted Disruption of the Osteoblast/Osteocyte Factor 45 Gene (OF45) Results in Increased Bone Formation and Bone Mass
- Author
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Amy L. Mansolf, Hua Zhu Ke, Lori C. Gowen, Hollis A. Simmons, George T. Tkalcevic, John D. McNeish, Thomas A. Brown, Kristen L. Chidsey-Frink, D.T. Crawford, Hong Qi, Jeffrey L. Stock, and Donna N. Petersen
- Subjects
medicine.medical_specialty ,DNA, Complementary ,Molecular Sequence Data ,Gene Expression ,Biochemistry ,Bone resorption ,Bone remodeling ,Osteoclast ,Internal medicine ,Bone cell ,medicine ,Animals ,Cloning, Molecular ,Molecular Biology ,Cells, Cultured ,DNA Primers ,Glycoproteins ,Extracellular Matrix Proteins ,Bone Development ,Base Sequence ,Chemistry ,Osteoblast ,Organ Size ,Cell Biology ,Phosphoproteins ,Rats ,Cell biology ,Bone morphogenetic protein 7 ,Phenotype ,medicine.anatomical_structure ,Endocrinology ,Osteocyte ,Cancellous bone - Abstract
We have previously described osteoblast/osteocyte factor 45 (OF45), a novel bone-specific extracellular matrix protein, and demonstrated that its expression is tightly linked to mineralization and bone formation. In this report, we have cloned and characterized the mouse OF45 cDNA and genomic region. Mouse OF45 (also called MEPE) was similar to its rat orthologue in that its expression was increased during mineralization in osteoblast cultures and the protein was highly expressed within the osteocytes that are imbedded within bone. To further determine the role of OF45 in bone metabolism, we generated a targeted mouse line deficient in this protein. Ablation of OF45 resulted in increased bone mass. In fact, disruption of only a single allele of OF45 caused significantly increased bone mass. In addition, knockout mice were resistant to aging-associated trabecular bone loss. Cancellous bone histomorphometry revealed that the increased bone mass was the result of increased osteoblast number and osteoblast activity with unaltered osteoclast number and osteoclast surface in knockout animals. Consistent with the bone histomorphometric results, we also determined that OF45 knockout osteoblasts produced significantly more mineralized nodules in ex vivo cell cultures than did wild type osteoblasts. Osteoclastogenesis and bone resorption in ex vivo cultures was unaffected by OF45 mutation. We conclude that OF45 plays an inhibitory role in bone formation in mouse.
- Published
- 2003
37. Molecular Characterization of the Substance P·Neurokinin-1 Receptor Complex
- Author
-
Amy L. Ulfers, Norman D. Boyd, Maria Pellegrini, Andrew A. Bremer, and Dale F. Mierke
- Subjects
Molecular dynamics ,Transmembrane domain ,Molecular model ,Photoaffinity labeling ,Chemistry ,Stereochemistry ,C-terminus ,Tachykinin receptor 1 ,Cell Biology ,Ligand (biochemistry) ,Receptor ,Molecular Biology ,Biochemistry - Abstract
Molecular models for the interaction of substance P (SP) with its G protein-coupled receptor, the neurokinin-1 receptor (NK-1R), have been developed. The ligand·receptor complex is based on experimental data from a series of photoaffinity labeling experiments and spectroscopic structural studies of extracellular domains of the NK-1R. Using the ligand/receptor contact points derived from incorporation of photolabile probes (p-benzoylphenylalanine (Bpa)) into SP at positions 3, 4, and 8 and molecular dynamics simulations, the topological arrangement of SP within the NK-1R is explored. The model incorporates the structural features, determined by high resolution NMR studies, of the second extracellular loop (EC2), containing contact points Met174 and Met181, providing important experimentally based conformational preferences for the simulations. Extensive molecular dynamics simulations were carried out to probe the nature of the two contact points identified for the Bpa3SP analogue (Bremer, A. A., Leeman, S. E., and Boyd, N. D. (2001) J. Biol. Chem. 276, 22857–22861), examining modes of ligand binding in which the contact points are fulfilled sequentially or simultaneously. The resulting ligand·receptor complex has the N terminus of SP projecting toward transmembrane helix (TM) 1 and TM2, exposed to the solvent. The C terminus of SP is located in proximity to TM5 and TM6, deeper into the central core of the receptor. The central portion of the ligand, adopting a helical loop conformation, is found to align with the helices of the central regions EC2 and EC3, forming important interactions with both of these extracellular domains. The model developed here allows for atomic insight into the biochemical data currently available and guides targeting of future experiments to probe specific ligand/receptor interactions and thereby furthers our understanding of the functioning of this important neuropeptide system.
- Published
- 2001
38. Phosphorylation of Ser363, Thr370, and Ser375 Residues within the Carboxyl Tail Differentially Regulates μ-Opioid Receptor Internalization
- Author
-
Ping-Yee Law, Amy L. Burd, Rachid El Kouhen, Laurie J. Erickson-Herbrandson, Horace H. Loh, and Chia Yu Chang
- Subjects
Threonine ,medicine.drug_class ,media_common.quotation_subject ,Molecular Sequence Data ,Receptors, Opioid, mu ,Diprenorphine ,Biology ,Transfection ,Biochemistry ,Cell Line ,Phosphates ,Phosphoserine ,chemistry.chemical_compound ,Opioid receptor ,Serine ,medicine ,Enzyme-linked receptor ,Animals ,Humans ,Amino Acid Sequence ,Phosphorylation ,Internalization ,Receptor ,Molecular Biology ,media_common ,Alanine ,Cell Biology ,Enkephalin, Ala(2)-MePhe(4)-Gly(5) ,Interleukin-13 receptor ,Molecular biology ,Endocytosis ,Recombinant Proteins ,Rats ,Kinetics ,DAMGO ,Phosphothreonine ,Amino Acid Substitution ,chemistry ,Mutagenesis, Site-Directed - Abstract
Prolonged activation of opioid receptors leads to their phosphorylation, desensitization, internalization, and down-regulation. To elucidate the relationship between mu-opioid receptor (MOR) phosphorylation and the regulation of receptor activity, a series of receptor mutants was constructed in which the 12 Ser/Thr residues of the COOH-terminal portion of the receptor were substituted to Ala, either individually or in combination. All these mutant constructs were stably expressed in human embryonic kidney 293 cells and exhibited similar expression levels and ligand binding properties. Among those 12 Ser/Thr residues, Ser(363), Thr(370), and Ser(375) have been identified as phosphorylation sites. In the absence of the agonist, a basal phosphorylation of Ser(363) and Thr(370) was observed, whereas [d-Ala(2),Me-Phe(4),Gly(5)-ol]enkephalin (DAMGO)-induced receptor phosphorylation occurs at Thr(370) and Ser(375) residues. Furthermore, the role of these phosphorylation sites in regulating the internalization of MOR was investigated. The mutation of Ser(375) to Ala reduced the rate and extent of receptor internalization, whereas mutation of Ser(363) and Thr(370) to Ala accelerated MOR internalization kinetics. The present data show that the basal phosphorylation of MOR could play a role in modulating agonist-induced receptor internalization kinetics. Furthermore, even though mu-receptors and delta-opioid receptors have the same motif encompassing agonist-induced phosphorylation sites, the different agonist-induced internalization properties controlled by these sites suggest differential cellular regulation of these two receptor subtypes.
- Published
- 2001
39. Demonstration of Conformational Changes Associated with Activation of the Maltose Transport Complex
- Author
-
Susan Sharma, Amy L. Davidson, and Daynene E. Mannering
- Subjects
Adenosine Triphosphatases ,Monosaccharide Transport Proteins ,Protein Conformation ,Chemistry ,Escherichia coli Proteins ,Binding protein ,Protein subunit ,Walker motifs ,Transporter ,Cell Biology ,Periplasmic space ,Biochemistry ,Maltose-Binding Proteins ,Transmembrane protein ,Spectrometry, Fluorescence ,Bacterial Proteins ,ATP hydrolysis ,Periplasmic Binding Proteins ,Solvents ,ATP-Binding Cassette Transporters ,Carrier Proteins ,Maltose ,Maltose transport complex ,Molecular Biology - Abstract
In Escherichia coli, interaction of a periplasmic maltose-binding protein with a membrane-associated ATP-binding cassette transporter stimulates ATP hydrolysis, resulting in translocation of maltose into the cell. The maltose transporter contains two transmembrane subunits, MalF and MalG, and two copies of a nucleotide-hydrolyzing subunit, MalK. Mutant transport complexes that function in the absence of binding protein are thought to be stabilized in an ATPase-active conformation. To probe the conformation of the nucleotide-binding site and to gain an understanding of the nature of the conformational changes that lead to activation, cysteine 40 within the Walker A motif of the MalK subunit was modified by the fluorophore 2-(4'-maleimidoanilino)naphthalene-6-sulfonic acid. Fluorescence differences indicated that residues involved in nucleotide binding were less accessible to aqueous solvent in the binding protein independent transporter than in the wild-type transporter. Similar differences in fluorescence were seen when a vanadate-trapped transition state conformation was compared with the ground state in the wild-type transporter. Our results and recent crystal structures are consistent with a model in which activation of ATPase activity is associated with conformational changes that bring the two MalK subunits closer together, completing the nucleotide-binding sites and burying ATP in the interface.
- Published
- 2001
40. Nucleophile Selection for the Endonuclease Activities of Human, Ovine, and Avian Retroviral Integrases
- Author
-
Malgorzata Sudol, Lynn M. Skinner, Amy L. Harper, and Michael Katzman
- Subjects
Glycerol ,Visna-maedi virus ,Visna virus ,HIV Integrase ,Biochemistry ,Catalysis ,Substrate Specificity ,Birds ,Endonuclease ,chemistry.chemical_compound ,Nucleophile ,Animals ,Humans ,Magnesium ,Molecular Biology ,chemistry.chemical_classification ,Manganese ,Rous sarcoma virus ,Sheep ,Integrases ,biology ,Hydrolysis ,Water ,Cell Biology ,Endonucleases ,biology.organism_classification ,Integrase ,Kinetics ,Retroviridae ,Enzyme ,Avian Sarcoma Viruses ,Oligodeoxyribonucleotides ,chemistry ,Alcohols ,DNA, Viral ,biology.protein ,DNA - Abstract
Retroviral integrases catalyze four endonuclease reactions (processing, joining, disintegration, and nonspecific alcoholysis) that differ in specificity for the attacking nucleophile and target DNA sites. To assess how the two substrates of this enzyme affect each other, we performed quantitative analyses, in three retroviral systems, of the two reactions that use a variety of nucleophiles. The integrase proteins of human immuno- deficiency virus type 1, visna virus, and Rous sarcoma virus exhibited distinct preferences for water or other nucleophiles during site-specific processing of viral DNA and during nonspecific alcoholysis of nonviral DNA. Although exogenous alcohols competed with water as the nucleophile for processing, the alcohols stimulated nicking of nonviral DNA. Moreover, different nucleophiles were preferred when the various integrases acted on different DNA targets. In contrast, the nicking patterns were independent of whether integrase was catalyzing hydrolysis or alcoholysis and were not influenced by the particular exogenous alcohol. Thus, although the target DNA influenced the choice of nucleophile, the nucleophile did not affect the choice of target sites. These results indicate that interaction with target DNA is the critical step before catalysis and suggest that integrase does not reach an active conformation until target DNA has bound to the enzyme.
- Published
- 2001
41. Identification of Osteoblast/Osteocyte Factor 45 (OF45), a Bone-specific cDNA Encoding an RGD-containing Protein That Is Highly Expressed in Osteoblasts and Osteocytes
- Author
-
George T. Tkalcevic, Thomas A. Brown, Donna N. Petersen, Ramon Rivera-Gonzalez, and Amy L. Mansolf
- Subjects
Molecular Sequence Data ,Osteocalcin ,Bone Marrow Cells ,Osteocytes ,Biochemistry ,Bone and Bones ,Bone remodeling ,Nucleotidases ,Complementary DNA ,medicine ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Molecular Biology ,Cells, Cultured ,Glycoproteins ,Extracellular Matrix Proteins ,Osteoblasts ,Base Sequence ,Sequence Homology, Amino Acid ,Tibia ,Chemistry ,Gene Expression Profiling ,Cell Differentiation ,Osteoblast ,Cell Biology ,Phosphoproteins ,Immunohistochemistry ,Molecular biology ,Rats ,Bone morphogenetic protein 7 ,medicine.anatomical_structure ,Organ Specificity ,Suppression subtractive hybridization ,Osteocyte ,MEPE ,Protein Expression Analysis ,Oligopeptides - Abstract
We describe the cloning and characterization of a novel bone-specific cDNA predicted to encode an extracellular matrix protein. This cDNA was identified by subtractive hybridization based upon its high expression in bone marrow-derived osteoblasts. By Northern blot analysis, we detected a single 2-kilobase mRNA transcript in bone, whereas no expression was detected in other tissues. Immunohistochemistry revealed that the protein was expressed highly in osteocytes within trabecular and cortical bone. RNA and protein expression analysis using in vivo marrow ablation as a model of bone remodeling demonstrated that this gene was expressed only in cells that were embedded within bone matrix in contrast to the earlier expression of known osteoblast markers. The cDNA was predicted to encode a serine/glycine-rich secreted peptide containing numerous potential phosphorylation sites and one RGD sequence motif. The interaction of RGD domain-containing peptides with integrins has been shown previously to regulate bone remodeling by promoting recruitment, attachment, and differentiation of osteoblasts and osteoclasts. Secretion of this RGD-containing protein from osteocytes has the potential to regulate cellular activities within the bone environment and thereby may impact bone homeostasis. We propose the name OF45 (osteoblast/osteocyte factor of 45 kDa) for this novel cDNA.
- Published
- 2000
42. Transcription Termination by RNA Polymerase III in Fission Yeast
- Author
-
Mitsuhiro Hamada, Shashi B. Koduru, Richard J. Maraia, and Amy L. Sakulich
- Subjects
Genetics ,biology ,Termination factor ,Saccharomyces cerevisiae ,Cell Biology ,biology.organism_classification ,Biochemistry ,RNA polymerase III ,TRNA transcription ,Cell biology ,chemistry.chemical_compound ,chemistry ,Transcription (biology) ,RNA polymerase ,Transfer RNA ,Schizosaccharomyces pombe ,Molecular Biology - Abstract
In order for RNA polymerase (pol) III to produce a sufficient quantity of RNAs of appropriate structure, initiation, termination, and reinitiation must be accurate and efficient. Termination-associated factors have been shown to facilitate reinitiation and regulate transcription in some species. Suppressor tRNA genes that differ in the dT(n) termination signal were examined for function in Schizosaccharomyces pombe. We also developed an S. pombe extract that is active for tRNA transcription that is described here for the first time. The ability of this tRNA gene to be transcribed in extracts from different species allowed us to compare termination in three model systems. Although human pol III terminates efficiently at 4 dTs and S. pombeat 5 dTs, Saccharomyces cerevisiae pol III requires 6 dTs to direct comparable but lower termination efficiency and also appears qualitatively distinct. Interestingly, this pattern of sensitivity to a minimal dT(n) termination signal was found to correlate with the sensitivity to α-amanitin, as S. pombe was intermediate between human and S. cerevisiae pols III. The results establish that the pols III of S. cerevisiae,S. pombe, and human exhibit distinctive properties and that termination occurs in S. pombe in a manner that is functionally more similar to human than is S. cerevisiae.
- Published
- 2000
43. Interaction of the N-Methyl-d-Aspartic Acid Receptor NR2D Subunit with the c-Abl Tyrosine Kinase
- Author
-
Maria R. Angiolieri, Robert T. Glover, Thomas E. Smithgall, Daniel T. Monaghan, Jean Y. J. Wang, Steven Kelly, and Amy L. Buller
- Subjects
Recombinant Fusion Proteins ,Protein subunit ,Immunoblotting ,Molecular Sequence Data ,Receptors, N-Methyl-D-Aspartate ,Biochemistry ,SH3 domain ,Cell Line ,src Homology Domains ,Epitopes ,FYN ,hemic and lymphatic diseases ,Escherichia coli ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Kinase activity ,Proto-Oncogene Proteins c-abl ,neoplasms ,Molecular Biology ,ABL ,biology ,Chemistry ,Autophosphorylation ,Cell Biology ,Precipitin Tests ,Molecular biology ,Mutagenesis, Site-Directed ,biology.protein ,GRB2 ,Proto-oncogene tyrosine-protein kinase Src - Abstract
The COOH-terminal domain of the NR2D subunit of the NMDA receptor contains proline-rich regions that show striking homology to sequences known to bind to Src homology 3 (SH3) domains. To determine whether the proline-rich region of the NR2D subunit interacts with specific SH3 domains, in vitro SH3 domain binding assays were performed. A proline-rich fragment of the NR2D subunit (2D(866-1064)) bound to the Abl SH3 domain but not to the SH3 domains from Src, Fyn, Grb2, GAP, or phospholipase C-gamma (PLCgamma). Co-immunoprecipitation of NR2D with Abl suggests stable association of NR2D and Abl in transfected cells. The SH3 domain plays an important role in the negative regulation of Abl kinase activity. To determine whether the interaction of NR2D with the Abl SH3 domain alters Abl kinase activity, Abl was expressed alone or with NR2D in 293T cells. Autophosphorylation of Abl was readily observed when Abl was expressed alone. However, co-expression of Abl with 2D(866-1064) or full-length NR2D inhibited autophosphorylation. 2D(866-1064) did not inhibit DeltaSH3 Abl, indicating a requirement for the Abl SH3 domain in the inhibitory effect. Similarly, 2D(866-1064) did not inhibit the catalytic activity of Abl-PP, which contains two point mutations in the SH2-kinase linker domain that release the negative kinase regulation by the SH3 domain. In contrast, the full-length NR2D subunit partially inhibited the autokinase activity of both DeltaSH3 Abl and Abl-PP, suggesting that NR2D and Abl may interact at multiple sites. Taken together, the data in this report provide the first evidence for a novel inhibitory interaction between the NR2D subunit of the NMDA receptor and the Abl tyrosine kinase.
- Published
- 2000
44. Photocross-linking of an Oriented DNA Repair Complex
- Author
-
Amy L. Kimzey, Sunghan Yoo, and William S. Dynan
- Subjects
Ku70 ,Ku80 ,Cell Biology ,Biology ,Biochemistry ,Free dna ,Ku Protein ,chemistry.chemical_compound ,Crystallography ,Protein structure ,chemistry ,DNA repair complex ,Biophysics ,Molecular Biology ,Groove (joinery) ,DNA - Abstract
Ku protein binds broken DNA ends, triggering a double-strand DNA break repair pathway. The spatial arrangement of the two Ku subunits in the initial Ku-DNA complex, when the Ku protein first approaches the broken DNA end, is not well defined. We have investigated the geometry of the complex using a novel set of photocross-linking probes that force Ku protein to be constrained in position and orientation, relative to a single free DNA end. Results suggest that this complex is roughly symmetric and that both Ku subunits make contact with an approximately equal area of the DNA. The complex has a strongly preferred orientation, with Ku70-DNA backbone contacts located proximal and Ku80-DNA backbone contacts located distal to the free end. Ku70 also contacts functional groups in the major groove proximal to the free end. Ku80 apparently does not make major groove contacts. Results are consistent with a model where the Ku70 and Ku80 subunits contact the major and minor grooves of DNA, respectively.
- Published
- 1999
45. Combinatorial Interactions Regulate Cardiac Expression of the Murine Adenylosuccinate Synthetase 1 Gene
- Author
-
Jeanie B. McMillin, Surjit K. Datta, Amy L. Lewis, Rodney E. Kellems, and Yang Xia
- Subjects
Transcriptional Activation ,Transgene ,DNA Mutational Analysis ,Mice, Transgenic ,Deoxyribonuclease HindIII ,Biology ,Biochemistry ,DNA-binding protein ,Gene Expression Regulation, Enzymologic ,Adenylosuccinate Synthase ,Mice ,Genes, Reporter ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,Deoxyribonucleases, Type II Site-Specific ,Promoter Regions, Genetic ,Enhancer ,Molecular Biology ,Transcription factor ,Gene ,In Situ Hybridization ,Genetics ,Myocardium ,Helix-Loop-Helix Motifs ,Heart ,Adenylosuccinate synthase ,Cell Biology ,Myocardial Contraction ,DNA-Binding Proteins ,DNA binding site ,biology.protein ,HAND2 ,Transcription Factors - Abstract
The mammalian heart begins contracting at the linear tube stage during embryogenesis and continuously pumps, nonstop, throughout the entire lifetime of the animal. Therefore, the cardiac energy metabolizing pathways must be properly established and efficiently functioning. While the biochemistry of these pathways is well defined, limited information regarding the regulation of cardiac metabolic genes is available. Previously, we reported that 1.9 kilobase pairs of murine adenylosuccinate synthetase 1 gene (Adss1) 5'-flanking DNA directs high levels of reporter expression to the adult transgenic heart. In this report, we define the 1.9-kilobase pair fragment as a cardiac-specific enhancer that controls correct spatiotemporal expression of a reporter similar to the endogenous Adss1 gene. A 700-base pair fragment within this region activates a heterologous promoter specifically in adult transgenic hearts. Proteins present in a cardiac nuclear extract interact with potential transcription factor binding sites of this region and these cis-acting sites play important regulatory roles in the cardiac expression of this reporter. Finally, we report that several different cardiac transcription factors trans-activate the 1.9HSCAT construct through these sites and that combinations result in enhanced reporter expression. Adss1 appears to be one of the first target genes identified for the bHLH factors Hand1 and Hand2.
- Published
- 1999
46. Identification of Serine 356 and Serine 363 as the Amino Acids Involved in Etorphine-induced Down-regulation of the μ-Opioid Receptor
- Author
-
Ping-Yee Law, Laurie J. Erickson, Amy L. Burd, Horace H. Loh, and Rachid El-Kouhen
- Subjects
Models, Molecular ,medicine.drug_class ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Receptors, Opioid, mu ,Diprenorphine ,Down-Regulation ,Glutamic Acid ,Biology ,Transfection ,Biochemistry ,Protein Structure, Secondary ,Cell Line ,Serine ,Opioid receptor ,Cyclic AMP ,medicine ,Humans ,5-HT5A receptor ,Amino Acid Sequence ,Receptor ,Molecular Biology ,Peptide sequence ,Sequence Deletion ,Colforsin ,Etorphine ,Cell Biology ,Mutagenesis, Site-Directed ,Phosphorylation ,Adenylyl Cyclases ,medicine.drug - Abstract
Agonist-induced internalization of G protein-coupled receptors is influenced by many structural determinants including the carboxyl tail. To investigate the role of serine and threonine residues within the carboxyl tail, several mutants were constructed by truncating the carboxyl tail of the hemagglutinin-tagged mu-opioid receptor, thereby removing serines and threonines systematically. Neuro2A cells stably expressing the truncated receptors did not exhibit a significant alteration in the affinity of [3H]diprenorphine or etorphine for the receptor or the potency of etorphine to inhibit forskolin-stimulated adenylyl cyclase activity. Chronic etorphine treatment resulted in a time-dependent down-regulation of all the truncated receptors, except MOR1TAG355D, thus revealing the importance of the four amino acids between Ser355 and Glu359 (STIE). Surprisingly, deletion of the STIE sequence resulted in a receptor that down-regulated the same as the wild-type receptor. The involvement of multiple amino acids within the carboxyl tail was demonstrated by combining alanine substitutions of several putative G-protein-coupled receptor kinase phosphorylation sites. Systematic analysis of these receptors indicated that mutation of Ser356 and Ser363 to alanine attenuated agonist-mediated down-regulation. The magnitude of etorphine-induced phosphorylation of this mutant receptor, however, was similar to that of the wild-type mu-opioid receptor. Thus, phosphorylation of the carboxyl tail of the mu-opioid receptor is not an obligatory event for etorphine-induced down-regulation of the receptor.
- Published
- 1998
47. Metabolism of Leukotriene C4 in γ-Glutamyl Transpeptidase-deficient Mice
- Author
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Bing Z. Carter, Ralph S. Orkiszewski, Amy L. Wiseman, Kevin D. Ballard, Michael W. Lieberman, and Ching Nan Ou
- Subjects
Leukotriene D4 ,Spectrometry, Mass, Fast Atom Bombardment ,Kidney ,digestive system ,Biochemistry ,Mice ,chemistry.chemical_compound ,medicine ,Animals ,Molecular Biology ,Acivicin ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Glutathione Disulfide ,Leukotriene C4 ,Molecular mass ,gamma-Glutamyltransferase ,Cell Biology ,Glutathione ,digestive system diseases ,Small intestine ,Papain ,Enzyme ,medicine.anatomical_structure ,chemistry - Abstract
We have investigated the metabolism of leukotriene C4 (LTC4) in gamma-glutamyl transpeptidase (GGT)-deficient mice (Lieberman, M. W., Wiseman, A. L., Shi, Z-Z., Carter, B. Z., Barrios, R., Ou, C-N., Chevez-Barrios, P., Wang, Y., Habib, G. M., Goodman, J. C., Huang, S. L., Lebovitz, R. M., and Matzuk, M. M. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 7923-7926) and have found substantial conversion of LTC4 to leukotriene D4 by high performance liquid chromatography and continuous flow fast atom bombardment-tandem mass spectrometric analyses. LTC4-converting activity has a tissue distribution different from GGT with highest activity in spleen followed by small intestine, kidney, and pancreas and lower activity in liver and lung. The activity is membrane-bound and is inhibited by acivicin, a known inhibitor of GGT. The enzyme was partially purified from the small intestine of GGT-deficient mice by papain treatment and gel filtration chromatography. The partially purified fragment released by papain has an apparent molecular mass of 65-70 kDa and the same substrate specificity as the tissue homogenate. In addition to LTC4, S-decyl-GSH is also cleaved. GSH itself, oxidized GSH, and the synthetic substrates used to analyze GGT activity (gamma-glutamyl-p-nitroanilide and gamma-glutamyl-4-methoxy-2-naphthylamide) are not substrates for this newly discovered enzyme. These data demonstrate that in addition to GGT at least one other enzyme cleaves LTC4 in mice. To reflect this enzyme's preferred substrate, we suggest that it be named gamma-glutamyl leukotrienase.
- Published
- 1997
48. Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene
- Author
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Oivin M. Guicherit, Surjit K. Datta, Rodney E. Kellems, Gerri Hanten, and Amy L. Lewis
- Subjects
Gene isoform ,Transcription, Genetic ,Transgene ,Molecular Sequence Data ,Mice, Transgenic ,Biochemistry ,Gene Expression Regulation, Enzymologic ,Adenylosuccinate Synthase ,Mice ,Open Reading Frames ,Gene expression ,medicine ,Animals ,Amino Acid Sequence ,Muscle, Skeletal ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,Base Sequence ,biology ,Adenine Nucleotides ,Myocardium ,Purine nucleotide cycle ,Cardiac muscle ,Chromosome Mapping ,Skeletal muscle ,Adenylosuccinate synthase ,Cell Biology ,Molecular biology ,medicine.anatomical_structure ,biology.protein - Abstract
A muscle-specific isoform of adenylosuccinate synthetase (AdSS1, EC) is one of three enzymes that constitute the purine nucleotide cycle, a muscle-specific metabolic cycle. Previously, we showed that the muscle Adss1 gene was highly expressed in both skeletal muscle and heart of the adult mouse. Here we have shown that the Adss1 gene is initially activated early in embryonic development in skeletal muscle and heart precursors and is subsequently up-regulated perinatally. The earliest detectable gene expression corresponds with the establishment of the first myogenic and cardiac lineages. To allow identification of the genetic signals controlling this developmental pattern of expression, the Adss1 gene was cloned and its structure determined. Transgenic analysis has shown that 1.9 kilobase pairs of 5′ flank can activate expression in skeletal muscle progenitors and direct enhanced expression to adult cardiac muscle. Sequence analysis of the promoter and 5′ flanking region revealed the presence of numerous potential muscle-specific cis-regulatory elements.
- Published
- 1996
49. Platelet-derived Growth Factor Induction of the Immediate-early gene Is Mediated by NF-κB and a 90-kDa Phosphoprotein Coactivator
- Author
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Amy L. Wrentmore, John A. Alberta, Charles D. Stiles, Grace Y. Hwang, and Rolf R. Freter
- Subjects
Reporter gene ,biology ,Cell Biology ,Biochemistry ,Molecular biology ,Transcription (biology) ,Phosphoprotein ,Coactivator ,biology.protein ,Binding site ,Molecular Biology ,Gene ,Immediate early gene ,Platelet-derived growth factor receptor - Abstract
A broad panel of agents including serum, interleukin-1, double-stranded RNA, and platelet-derived growth factor (PDGF) stimulate transcription of the “slow” immediate-early gene MCP-1. These disparate inducers act through a tight cluster of regulatory elements in the distal 5′-flanking sequences of the MCP-1 gene. We describe a 22-base element in this cluster which, in single copy, confers PDGF-inducibility to a tagged MCP-1 reporter gene. In mobility shift assays, the element binds a PDGF-activated form of NF-κB, and a 90-kDa protein (p90) which binds constitutively. Antibody supershift and UV cross-linking experiments indicate that the PDGF-activated NF-κB species is a Rel A homodimer. The DNA binding form of p90 is a nuclear-restricted serine/threonine phosphoprotein. Mutagenesis of the 22-base element shows that the NF-κB and p90 binding sites overlap, but binding of the two species is mutually independent. Both sites, however, are required for optimum PDGF induction of MCP-1. Therefore, p90 appears to be a coactivator with NF-κB in PDGF-mediated induction of MCP-1.
- Published
- 1996
50. Maltose-binding Protein Containing an Interdomain Disulfide Bridge Confers a Dominant-negative Phenotype for Transport and Chemotaxis
- Author
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Amy L. Davidson, Yinghua Zhang, Michael D. Manson, Nanhua Yao, and Daynene E. Mannering
- Subjects
Monosaccharide Transport Proteins ,Protein Conformation ,Biochemistry ,Maltose-Binding Proteins ,Dithiothreitol ,chemistry.chemical_compound ,Maltose-binding protein ,Protein structure ,Bacterial Proteins ,Escherichia coli ,Cysteine ,Disulfides ,Maltose ,Molecular Biology ,Maltose transport ,biology ,Chemotaxis ,Escherichia coli Proteins ,Binding protein ,Mutagenesis ,Biological Transport ,Cell Biology ,Phenotype ,chemistry ,biology.protein ,Biophysics ,ATP-Binding Cassette Transporters ,Carrier Proteins - Abstract
Bacterial substrate-binding proteins exist in an equilibrium among four forms: open/substrate-free, open/substrate-bound, closed/substrate-free, and closed/substrate-bound. Ligands stabilize the closed conformation, whereas the open conformation predominates in the substrate-free species. In its closed form, the NH2-terminal and COOH-terminal domains of maltose-binding protein (MBP) are proposed to be aligned to allow residues in both domains to interact simultaneously with complementary sites on the MalF and MalG proteins of the maltodextrin uptake system or with the Tar chemotactic signal transducer. However, the initial interaction might occur with an open/substrate-bound form of the binding protein, which would then close in contact with MalFG or Tar. Ligand would help stabilize this complex. We introduced cysteines (G69C and S337C) by site-directed mutagenesis into each domain of MBP and found that they formed an interdomain disulfide cross-link that should hold the protein in a closed conformation. This mutant MBP confers a dominant-negative phenotype for growth on maltose, for maltose transport, and for maltose chemotaxis. The growth and transport defects are partially reversed when the cells are exposed to the reducing agent dithiothreitol. We conclude that the cross-linked form of MBP competes with wild-type MBP in vivo for interaction with MalFG and Tar.
- Published
- 1996
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