1. Possible Implications of the Induction of Human Heme Oxygenase-1 by Nitric Oxide Donors1.
- Author
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Takahashi, Kazuhiro, Hara, Eishi, Ogawa, Kazuhiro, Kimura, Dai, Fujita, Hiroyoshi, and Shibahara, Shigeki
- Subjects
NITRIC oxide ,OXYGENASES ,CERVICAL cancer ,SODIUM nitroferricyanide ,GLUTATHIONE - Abstract
To explore the involvement of nitric oxide (NO) in the induction of heme oxygenase-1, an essential enzyme in heme catabolism, we studied the effects of NO donors on the expression of heme oxygenase-1 mRNA in HeLa human cervical cancer cells. Treatment with each of three NO donors, sodium nitroprusside, 3-morpholinosydnonimine, and S-nitroso-L-glutathione, caused noticeable increases in the expression levels of heme oxygenase-1 mRNA, but not heme oxygenase-2 mRNA. On the other hand, nitrite or 8-bromo cGMP exerted no noticeable effect on the levels of heme oxygenase-1 mRNA. We showed that sodium nitroprusside also increased the levels of heme oxygenase-1 protein. The sodium nitroprusside-mediated increase in heme oxygenase-1 mRNA levels was abolished by treatment with actinomycin D. The expression levels of heme oxygenase-1 mRNA were also increased by NO donors in human melanoma and neuroblastoma cell lines. Thus, the observed induction of heme oxygenase-1 may represent an important response to NO or NO-related oxidative stress. The half lives of heme oxygenase-1 and heme oxygenase-2 mRNAs were estimated to be about 3.2 h and more than 5 h, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 1997
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