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Objective To identify the chemical components of Guanxin H formula, and explore its mechanism in the treatment of acute myocardial infarction. Methods In this study, the components of Guanxin H formula were identified w让h high performance liquid chromatography-mass spectrometrytandem mass spectrometry (HPLC-MS/MS), the targets of the detected components were screened by searching the TCMSP database, and the disease targets were screened by searching the GeneCards and OMIM databases. Then STRING was applied to build the network of PPI, and Bioconductor database was used to perform GO and KEGG enrichment analysis, and Cytoscape software was used to build the drug・ component-target-pathway-disease network diagram・ Core targets were then obtained by calculating topological parameters・ Finally, detected components and targets were identified by molecular docking・ Results Five chemical components of Guanxin H formula were detected, i・ e. ferulic acid, tanshinol, paeoniflorin, protocatechualdehyde, and hydroxysafflor yellow A (HSYA)・ Nine key targets were screened, including CHRM1, CHRM2, CHRM3, PTGS1, ADRA1A, PLAU, GABRA1, AR and IL6, with the neuroactive ligand-receptor interaction pathway, calcium signaling pathway, cholinergic synapse pathway, and actin cytoskeleton signaling pathway involved・ Molecular docking showed good binding activity between the detected components and CHRM1, and the molecular conformation was stable・ Conclusion Five quality control components of Guanxin H formula could be detected relatively quickly and accurately by HPLC-MS/MS・ By binding with CHRM1, the components of Guanxin H seem to protect cardiac myocytes through regulating the neuroactive ligand-receptor interaction pathway, calcium signaling pathway, cholinergic synapse pathway, and actin cytoskeleton signaling pathway, thus inhibiting oxidative stress, inflammatory damage and apoptosis after myocardial infarction. This study potentially provides a new idea and direction for further exploring the mechanism of Guanxin H formula in the treatment of acute myocardial infarction. However, further experimental proof is still needed to determine whether Guanxin H formula plays a role through the four pathways above, and to clarify the main targets and interaction between pathways. [ABSTRACT FROM AUTHOR]