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Your search keyword '"van Putten, Jos P M"' showing total 10 results
Start Over Author "van Putten, Jos P M" Journal journal of bacteriology
10 results on '"van Putten, Jos P M"'

1. Nucleases encoded by the integrated elements CJIE2 and CJIE4 inhibit natural transformation of campylobacter jejuni

Author

Gaasbeek, Esther J., Wagenaar, Jaap A., Guilhabert, Magalie R., van Putten, Jos P. M., Parker, Craig T., and van der Wal, Fimme J.

Subjects
Campylobacter -- Genetic aspects, Campylobacter -- Research, Nucleases -- Research, Bacterial transformation -- Research, Biological sciences
Abstract

The species Campylobacter jejuni is naturally competent for DNA uptake; nevertheless, nonnaturally transformable strains do exist. For a subset of strains we previously showed that a periplasmic DNase, encoded by dns, inhibits natural transformation in C. jejuni. In the present study, genetic factors coding for DNase activity in the absence of dns were identified. DNA arrays indicated that nonnaturally transformable dns-negative strains contain putative DNA/RNA nonspecific endonucleases encoded by CJE0566 and CJE1441 of strain RM1221. These genes are located on C. jejuni integrated elements 2 and 4. Expression of CJE0566 and CJE1441 from strain RM1221 and a homologous gene from strain 07479 in DNase-negative Escherichia coil and C. jejuni strains indicated that these genes code for DNases. Genetic transfer of the genes to a naturally transformable C. jejuni strain resulted in a decreased efficiency of natural transformation. Modeling suggests that the C. jejuni DNases belong to the Serratia nuclease family. Overall, the data indicate that the acquisition of prophageencoded DNA/RNA nonspecific endonucleases inhibits the natural transformability of C. jejuni through hydrolysis of DNA. doi:10.1128/JB.00867-09

Published
2010

2. Nucleases encoded by the integrated elements CJIE2 and CJIE4 inhibit natural transformation of Campylobacter jejuni

Author

Gaasbeek, Esther J, Wagenaar, Jaap A, Guilhabert, Magalie R, van Putten, Jos P M, Parker, Craig T, van der Wal, Fimme J, LS Klinisch Onderzoek Wagenaar, Faculteit Diergeneeskunde, and LS Infectiebiologie (Bacteriologie)

Subjects
Models, Molecular, Serratia, Genes, Viral, Prophages, medicine.disease_cause, chemistry.chemical_compound, Models, Viral, bacteria, Genetics, Deoxyribonucleases, horizontal gene-transfer, Genetic transfer, Bacterial, RNA, Bacterial, mutagenesis, ID - Infectieziekten, DNA, Bacterial, Protein Structure, Coronacrisis-Taverne, mechanism, Biology, Microbiology, Campylobacter jejuni, diversity, Transformation, strains, Microbial Cell Biology, Bacterial Proteins, serratia-marcescens endonuclease, medicine, Escherichia coli, Molecular Biology, Gene, Prophage, dnase, Gene Expression Profiling, RNA, Molecular, DNA, sequence, biology.organism_classification, Protein Structure, Tertiary, Transformation (genetics), chemistry, Genes, Genes, Bacterial, CVI - Divisie Bacteriologie en TSE's, identification, Transformation, Bacterial, Tertiary
Abstract

The species Campylobacter jejuni is naturally competent for DNA uptake; nevertheless, nonnaturally transformable strains do exist. For a subset of strains we previously showed that a periplasmic DNase, encoded by dns , inhibits natural transformation in C. jejuni . In the present study, genetic factors coding for DNase activity in the absence of dns were identified. DNA arrays indicated that nonnaturally transformable dns -negative strains contain putative DNA/RNA nonspecific endonucleases encoded by CJE0566 and CJE1441 of strain RM1221. These genes are located on C. jejuni integrated elements 2 and 4. Expression of CJE0566 and CJE1441 from strain RM1221 and a homologous gene from strain 07479 in DNase-negative Escherichia coli and C. jejuni strains indicated that these genes code for DNases. Genetic transfer of the genes to a naturally transformable C. jejuni strain resulted in a decreased efficiency of natural transformation. Modeling suggests that the C. jejuni DNases belong to the Serratia nuclease family. Overall, the data indicate that the acquisition of prophage-encoded DNA/RNA nonspecific endonucleases inhibits the natural transformability of C. jejuni through hydrolysis of DNA.

Published
2009

10. CJIE1 Inhibits Natural Transformation of Campylobacter jejuni.

Author

Gaasbeek, Esther J., Wagenaar, Jaap A., Guilhabert, Magalie R., Wösten, Marc M. S. M., van Putten, Jos P. M., van der Graaf-van Bloois, Linda, Parker, Craig T., and van der Wal, Fimme J.

Subjects
*CAMPYLOBACTER jejuni, *HYDROLYSIS, *PLASMID genetics, *GENETIC mutation, *NUCLEIC acid hybridization, *MOBILE genetic elements
Abstract

The species Campylobacter jejuni is considered naturally competent for DNA uptake and displays strong genetic diversity. Nevertheless, nonnaturally transformable strains and several relatively stable clonal lineages exist. In the present study, the molecular mechanism responsible for the nonnatural transformability of a subset of C. jejuni strains was investigated. Comparative genome hybridization indicated that C. jejuni Mu-like prophage integrated element 1 (CJIE1) was more abundant in nonnaturally transformable C. jejuni strains than in naturally transformable strains. Analysis of CJIE1 indicated the presence of dns (CJE0256), which is annotated as a gene encoding an extracellular DNase. DNase assays using a defined dns mutant and a dns-negative strain expressing Dns from a plasmid indicated that Dns is an endogenous DNase. The DNA-hydrolyzing activity directly correlated with the natural transformability of the knockout mutant and the dns-negative strain expressing Dns from a plasmid. Analysis of a broader set of strains indicated that the majority of nonnaturally transformable strains expressed DNase activity, while all naturally competent strains lacked this activity. The inhibition of natural transformation in C. jejuni via endogenous DNase activity may contribute to the formation of stable lineages in the C. jejuni population. [ABSTRACT FROM AUTHOR]

Published
2009
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