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Start Over Author "Price, A. E." Journal journal of bacteriology
11 results on '"Price, A. E."'

2. Pneumolysin localizes to the cell wall of Streptococcus pneumoniae

Author

Price, Katherine E. and Camilli, Andrew

Subjects
Streptococcus pneumoniae -- Genetic aspects, Streptococcus pneumoniae -- Research, Bacterial toxins -- Genetic aspects, Bacterial toxins -- Physiological aspects, Bacterial toxins -- Research, Cell fractionation -- Usage, Western immunoblotting -- Usage, Biological sciences
Abstract

Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates ofS. pneumoniae, and strains in which the Ply gene has been deleted are severely attenuated in mouse models of infection. In contrast to all other members of the cholesterol-dependent cytolysin family, Ply lacks a signal peptide for export. Instead, Ply has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanism that remains ill defined. We determined by use of cell fractionation and Western blotting that, during in vitro growth, exported Ply is localized primarily to the cell wall compartment in 18 different serotypes in the absence of detectable cell lysis. Hemolytic assays revealed that this cell wall-localized Ply is active. Additionally, cell wall-localized Ply is accessible to extracellular protease and is detergent releasable.

Published
2009

4. Altered Stool Microbiota of Infants with Cystic Fibrosis Shows a Reduction in Genera Associated with Immune Programming from Birth

Author

Antosca, Katherine M., primary, Chernikova, Diana A., additional, Price, Courtney E., additional, Ruoff, Kathryn L., additional, Li, Kewei, additional, Guill, Margaret F., additional, Sontag, Natalie R., additional, Morrison, Hilary G., additional, Hao, Shuyu, additional, Drumm, Mitchell L., additional, MacKenzie, Todd A., additional, Dorman, Dana B., additional, Feenan, Lynn M., additional, Williams, Molly A., additional, Dessaint, John, additional, Yuan, Irene H., additional, Aldrich, Brian J., additional, Moulton, Lisa A., additional, Ting, Lily, additional, Martinez-del Campo, Ana, additional, Stewart, Edward J., additional, Karagas, Margaret R., additional, O’Toole, George A., additional, and Madan, Juliette C., additional

Published
2019
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