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A study which was aimed at characterizing new Thermus strains and confirming the presence of glycerol-linked and diol-linked glycolipids within these strains was conducted. The methods used were extraction and purification of polar lipids, hydrolysis and analysis of glycolipids, preparation of fatty acid methyl esters (FAMEs) and derivatization. Results showed that partial structures of intact diol-containing lipids were present in the bacteria and some Thermus strains contain a mixture of diol- and glycerol-based glycolipids which were not present in the Thermomicrobium roseum.

The compositions of the primary glycolipids (GL-1) of five strains of Thermus aquaticus, the type strain of T. filiformis, T. oshimai SPS-11 and Thermus sp. strain CG-2 were studied. With the exception of T. aquaticus 15004, the organisms each have a major glycolipid whose structure was identified as diglycosyl-(N-acyl)glycosaminyl-glycosyl diacylglycerol. The unique major glycolipid of T. aquaticus 15004 was established as galactofuranosyl-(N-acetyl)galactosaminyl-(N-acyl)galactosaminyl-glucosyl diacylglycerol.

Examines the accumulation of intracellular organic solutes in six species of the order `Thermotogales' by nuclear magnetic resonance spectroscopy. Identification of organic solutes in `T. maritima'; Organic solutes in other members of the order `Thermotogales.'